G3ST4_HUMAN
ID G3ST4_HUMAN Reviewed; 486 AA.
AC Q96RP7; A4D2A8; B4DWL8; D6W5U5; Q8N3P7; Q8WZ17; Q96E33; Q9HA78;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Galactose-3-O-sulfotransferase 4;
DE Short=Gal3ST-4;
DE EC=2.8.2.-;
DE AltName: Full=Beta-galactose-3-O-sulfotransferase 4;
DE AltName: Full=Gal-beta-1,3-GalNAc 3'-sulfotransferase;
GN Name=GAL3ST4; ORFNames=PP6968;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=11333265; DOI=10.1074/jbc.m101558200;
RA Seko A., Hara-Kuge S., Yamashita K.;
RT "Molecular cloning and characterization of a novel human galactose 3-O-
RT sulfotransferase that transfers sulfate to Galbeta1->3GalNAc residue in O-
RT glycans.";
RL J. Biol. Chem. 276:25697-25704(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT VAL-467.
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X.,
RA Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.,
RA Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Mammary gland;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Amygdala;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT VAL-467.
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Catalyzes the transfer of sulfate to beta-1,3-linked
CC galactose residues in O-linked glycoproteins. Good substrates include
CC asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-
CC 1,6)GalNAc. {ECO:0000269|PubMed:11333265}.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 6-7.;
CC -!- PATHWAY: Protein modification; carbohydrate sulfation.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane
CC {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96RP7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96RP7-2; Sequence=VSP_057006, VSP_057007;
CC -!- TISSUE SPECIFICITY: Expressed mainly in placenta, thymus, testis,
CC ovary, spinal cord, trachea and adrenal gland and at low levels in
CC brain, lung, spleen, prostate, small intestine, colon, stomach thyroid
CC and lymph node. {ECO:0000269|PubMed:11333265}.
CC -!- SIMILARITY: Belongs to the galactose-3-O-sulfotransferase family.
CC {ECO:0000305}.
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DR EMBL; AF316113; AAK73365.1; -; mRNA.
DR EMBL; AF289575; AAL55759.1; -; mRNA.
DR EMBL; AK022178; BAB13977.1; -; mRNA.
DR EMBL; AK301591; BAG63080.1; -; mRNA.
DR EMBL; AL833824; CAD38686.2; -; mRNA.
DR EMBL; AC073842; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH236956; EAL23847.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76575.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76577.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76579.1; -; Genomic_DNA.
DR EMBL; BC012976; AAH12976.1; -; mRNA.
DR CCDS; CCDS5688.1; -. [Q96RP7-1]
DR RefSeq; NP_078913.3; NM_024637.4. [Q96RP7-1]
DR AlphaFoldDB; Q96RP7; -.
DR BioGRID; 122811; 3.
DR IntAct; Q96RP7; 1.
DR STRING; 9606.ENSP00000353142; -.
DR GlyGen; Q96RP7; 1 site.
DR iPTMnet; Q96RP7; -.
DR PhosphoSitePlus; Q96RP7; -.
DR BioMuta; GAL3ST4; -.
DR DMDM; 47116570; -.
DR EPD; Q96RP7; -.
DR jPOST; Q96RP7; -.
DR MassIVE; Q96RP7; -.
DR PaxDb; Q96RP7; -.
DR PeptideAtlas; Q96RP7; -.
DR PRIDE; Q96RP7; -.
DR ProteomicsDB; 5356; -.
DR ProteomicsDB; 77998; -. [Q96RP7-1]
DR Antibodypedia; 30607; 201 antibodies from 24 providers.
DR DNASU; 79690; -.
DR Ensembl; ENST00000360039.9; ENSP00000353142.4; ENSG00000197093.11. [Q96RP7-1]
DR Ensembl; ENST00000413800.5; ENSP00000400451.1; ENSG00000197093.11. [Q96RP7-1]
DR GeneID; 79690; -.
DR KEGG; hsa:79690; -.
DR MANE-Select; ENST00000360039.9; ENSP00000353142.4; NM_024637.5; NP_078913.3.
DR UCSC; uc003utt.4; human. [Q96RP7-1]
DR CTD; 79690; -.
DR DisGeNET; 79690; -.
DR GeneCards; GAL3ST4; -.
DR HGNC; HGNC:24145; GAL3ST4.
DR HPA; ENSG00000197093; Tissue enhanced (skin).
DR MIM; 608235; gene.
DR neXtProt; NX_Q96RP7; -.
DR NIAGADS; ENSG00000197093; -.
DR OpenTargets; ENSG00000197093; -.
DR PharmGKB; PA134921067; -.
DR VEuPathDB; HostDB:ENSG00000197093; -.
DR eggNOG; ENOG502QSHR; Eukaryota.
DR GeneTree; ENSGT00950000182923; -.
DR HOGENOM; CLU_040616_1_2_1; -.
DR InParanoid; Q96RP7; -.
DR OMA; NTTRMSC; -.
DR OrthoDB; 1385827at2759; -.
DR PhylomeDB; Q96RP7; -.
DR TreeFam; TF314802; -.
DR PathwayCommons; Q96RP7; -.
DR SignaLink; Q96RP7; -.
DR UniPathway; UPA00353; -.
DR BioGRID-ORCS; 79690; 15 hits in 1069 CRISPR screens.
DR GeneWiki; GAL3ST4; -.
DR GenomeRNAi; 79690; -.
DR Pharos; Q96RP7; Tdark.
DR PRO; PR:Q96RP7; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q96RP7; protein.
DR Bgee; ENSG00000197093; Expressed in parotid gland and 153 other tissues.
DR ExpressionAtlas; Q96RP7; baseline and differential.
DR Genevisible; Q96RP7; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0016020; C:membrane; IDA:UniProtKB.
DR GO; GO:0050656; F:3'-phosphoadenosine 5'-phosphosulfate binding; NAS:UniProtKB.
DR GO; GO:0050694; F:galactose 3-O-sulfotransferase activity; IDA:UniProtKB.
DR GO; GO:0001733; F:galactosylceramide sulfotransferase activity; IEA:InterPro.
DR GO; GO:0050698; F:proteoglycan sulfotransferase activity; NAS:UniProtKB.
DR GO; GO:0008146; F:sulfotransferase activity; IBA:GO_Central.
DR GO; GO:0007267; P:cell-cell signaling; NAS:UniProtKB.
DR GO; GO:0009247; P:glycolipid biosynthetic process; IEA:InterPro.
DR GO; GO:0009101; P:glycoprotein biosynthetic process; IDA:MGI.
DR GO; GO:0009100; P:glycoprotein metabolic process; NAS:UniProtKB.
DR GO; GO:0009311; P:oligosaccharide metabolic process; NAS:UniProtKB.
DR GO; GO:0030166; P:proteoglycan biosynthetic process; NAS:UniProtKB.
DR GO; GO:0006790; P:sulfur compound metabolic process; NAS:UniProtKB.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR009729; Gal-3-0_sulfotransfrase.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR14647; PTHR14647; 1.
DR Pfam; PF06990; Gal-3-0_sulfotr; 2.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Golgi apparatus; Membrane;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..486
FT /note="Galactose-3-O-sulfotransferase 4"
FT /id="PRO_0000085209"
FT TOPO_DOM 1..18
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 19..39
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 40..486
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 374
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..80
FT /note="MGPLSPARTLRLWGPRSLGVALGVFMTIGFALQLLGGPFQRRLPGLQLRQPS
FT APSLRPALPSCPPRQRLVFLKTHKSGSS -> MTPRSPTSAFRSCTAVSSCLCPSSWPW
FT LHLSVTSPFPVPHGPSLSCQDAAALGTSEPGGGSGSLHDHWLCTPALGRALPE (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_057006"
FT VAR_SEQ 81..142
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_057007"
FT VARIANT 353
FT /note="R -> Q (in dbSNP:rs3800952)"
FT /id="VAR_021989"
FT VARIANT 467
FT /note="A -> V (in dbSNP:rs3823646)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:15498874"
FT /id="VAR_033736"
FT CONFLICT 137
FT /note="M -> L (in Ref. 3; BAB13977)"
FT /evidence="ECO:0000305"
FT CONFLICT 201..204
FT /note="RGDH -> VGTT (in Ref. 2; AAL55759)"
FT /evidence="ECO:0000305"
FT CONFLICT 441
FT /note="S -> R (in Ref. 3; BAB13977)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 486 AA; 54166 MW; 2D731D16B191CC3C CRC64;
MGPLSPARTL RLWGPRSLGV ALGVFMTIGF ALQLLGGPFQ RRLPGLQLRQ PSAPSLRPAL
PSCPPRQRLV FLKTHKSGSS SVLSLLHRYG DQHGLRFALP ARYQFGYPKL FQASRVKGYR
PQGGGTQLPF HILCHHMRFN LKEVLQVMPS DSFFFSIVRD PAALARSAFS YYKSTSSAFR
KSPSLAAFLA NPRGFYRPGA RGDHYARNLL WFDFGLPFPP EKRAKRGNIH PPRDPNPPQL
QVLPSGAGPR AQTLNPNALI HPVSTVTDHR SQISSPASFD LGSSSFIQWG LAWLDSVFDL
VMVAEYFDES LVLLADALCW GLDDVVGFMH NAQAGHKQGL STVSNSGLTA EDRQLTARAR
AWNNLDWALY VHFNRSLWAR IEKYGQGRLQ TAVAELRARR EALAKHCLVG GEASDPKYIT
DRRFRPFQFG SAKVLGYILR SGLSPQDQEE CERLATPELQ YKDKLDAKQF PPTVSLPLKT
SRPLSP
