ID   G3_VACCW                Reviewed;         111 AA.
AC   P68458; P21024; Q76ZU3;
DT   23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Protein G3;
GN   OrderedLocusNames=VACWR079; ORFNames=G3L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2024483; DOI=10.1016/0042-6822(91)90585-y;
RA   Meis R.J., Condit R.C.;
RT   "Genetic and molecular biological characterization of a vaccinia virus gene
RT   which renders the virus dependent on isatin-beta-thiosemicarbazone (IBT).";
RL   Virology 182:442-454(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   IDENTIFICATION IN THE ENTRY-FUSION COMPLEX.
RX   PubMed=16339313; DOI=10.1073/pnas.0509239102;
RA   Senkevich T.G., Ojeda S., Townsley A., Nelson G.E., Moss B.;
RT   "Poxvirus multiprotein entry-fusion complex.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:18572-18577(2005).
RN   [4]
RP   INTERACTION WITH L5.
RX   PubMed=21295816; DOI=10.1016/j.virol.2011.01.014;
RA   Wolfe C.L., Moss B.;
RT   "Interaction between the G3 and L5 proteins of the vaccinia virus entry-
RT   fusion complex.";
RL   Virology 412:278-283(2011).
CC   -!- FUNCTION: Envelope protein part of the entry-fusion complex responsible
CC       for the virus membrane fusion with host cell membrane during virus
CC       entry. Also plays a role in cell-cell fusion (syncytium formation)
CC       (Probable). {ECO:0000305}.
CC   -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at least
CC       composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation
CC       of the viral membrane is necessary for the assembly of the complex.
CC       Interacts with L5. {ECO:0000269|PubMed:16339313,
CC       ECO:0000269|PubMed:21295816}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}. Note=Component of the mature virion
CC       (MV) membrane. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae G3 family. {ECO:0000305}.
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DR   EMBL; J03399; AAB59812.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89358.1; -; Genomic_DNA.
DR   RefSeq; YP_232961.1; NC_006998.1.
DR   SMR; P68458; -.
DR   TCDB; 1.G.11.1.1; the poxvirus cell entry protein complex (pep-c) family.
DR   DNASU; 3707535; -.
DR   GeneID; 3707535; -.
DR   KEGG; vg:3707535; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR010367; Poxvirus_G3.
DR   Pfam; PF06129; Chordopox_G3; 1.
PE   1: Evidence at protein level;
KW   Fusion of virus membrane with host cell membrane;
KW   Fusion of virus membrane with host membrane; Membrane; Reference proteome;
KW   Signal-anchor; Transmembrane; Transmembrane helix; Viral envelope protein;
KW   Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT   CHAIN           1..111
FT                   /note="Protein G3"
FT                   /id="PRO_0000099528"
FT   TRANSMEM        1..21
FT                   /note="Helical; Signal-anchor"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        22..111
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   111 AA;  12803 MW;  9BCD24ABADC2B07D CRC64;
     MASLLYLILF LLFVCISYYF TYYPTNKLQA AVMETDRENA IIRQRNDEIP TRTLDTAIFT
     DASTVASAQI HLYYNSNIGK IIMSLNGKKH TFNLYDDNDI RTLLPILLLS K