G4DT_SOYBN
ID G4DT_SOYBN Reviewed; 409 AA.
AC B9A1Q4;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=Glycinol 4-dimethylallyltransferase;
DE EC=2.5.1.36;
DE AltName: Full=Dimethylallyl diphosphate:(6aS,11aS)-(-)-3,9,6a-trihydroxypterocarpan ((-)-glycinol) 4-dimethylallyltransferase;
DE AltName: Full=Glyceollin synthase;
DE AltName: Full=Pterocarpan 4-dimethylallyltransferase;
DE Flags: Precursor;
GN Name=G4DT; OrderedLocusNames=Glyma10g44170; ORFNames=Gma.5621;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR,
RP BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, AND INDUCTION.
RC TISSUE=Protoplast;
RX PubMed=19091879; DOI=10.1104/pp.108.123679;
RA Akashi T., Sasaki K., Aoki T., Ayabe S., Yazaki K.;
RT "Molecular cloning and characterization of a cDNA for pterocarpan 4-
RT dimethylallyltransferase catalyzing the key prenylation step in the
RT biosynthesis of glyceollin, a soybean phytoalexin.";
RL Plant Physiol. 149:683-693(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82;
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
CC -!- FUNCTION: Proposed to be involved in the biosynthesis of pterocarpan
CC phytoalexins, specifically glyceollins. Can act as a prenyltransferase
CC towards glycinol which is the direct precursor of glyceollins. Seems to
CC be specific for prenylation at C-4 thus producing glyceollin I.
CC {ECO:0000269|PubMed:19091879}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6aS,11aS)-3,6a,9-trihydroxypterocarpan + dimethylallyl
CC diphosphate = (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan
CC + diphosphate; Xref=Rhea:RHEA:19345, ChEBI:CHEBI:15649,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:50118, ChEBI:CHEBI:57623; EC=2.5.1.36;
CC Evidence={ECO:0000269|PubMed:19091879};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6aS,11aS)-3,6a,9-trihydroxypterocarpan + dimethylallyl
CC diphosphate = (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
CC + diphosphate; Xref=Rhea:RHEA:23184, ChEBI:CHEBI:15649,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:50036, ChEBI:CHEBI:57623; EC=2.5.1.36;
CC Evidence={ECO:0000269|PubMed:19091879};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:19091879};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000269|PubMed:19091879};
CC Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC Evidence={ECO:0000269|PubMed:19091879};
CC Note=Magnesium or manganese or cobalt at a lesser extent.
CC {ECO:0000269|PubMed:19091879};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=45 uM for (-)-glycinol {ECO:0000269|PubMed:19091879};
CC KM=180 uM for dimethylallyl diphosphate
CC {ECO:0000269|PubMed:19091879};
CC -!- PATHWAY: Phytoalexin biosynthesis; pterocarpan phytoalexin
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000305|PubMed:19091879}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:19091879}.
CC -!- INDUCTION: Upon treatment with yeast extract.
CC {ECO:0000269|PubMed:19091879}.
CC -!- SIMILARITY: Belongs to the UbiA prenyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB434690; BAH22520.1; -; mRNA.
DR EMBL; CM000843; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001235990.1; NM_001249061.1.
DR AlphaFoldDB; B9A1Q4; -.
DR SMR; B9A1Q4; -.
DR STRING; 3847.GLYMA10G44170.1; -.
DR PRIDE; B9A1Q4; -.
DR EnsemblPlants; KRH36303; KRH36303; GLYMA_10G295300.
DR GeneID; 100301896; -.
DR Gramene; KRH36303; KRH36303; GLYMA_10G295300.
DR KEGG; gmx:100301896; -.
DR eggNOG; ENOG502R0I3; Eukaryota.
DR HOGENOM; CLU_048963_0_1_1; -.
DR InParanoid; B9A1Q4; -.
DR OMA; HAIMAGI; -.
DR OrthoDB; 1231215at2759; -.
DR BRENDA; 2.5.1.36; 2483.
DR UniPathway; UPA00901; -.
DR Proteomes; UP000008827; Chromosome 10.
DR Genevisible; B9A1Q4; GM.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004659; F:prenyltransferase activity; IEA:InterPro.
DR GO; GO:0047292; F:trihydroxypterocarpan dimethylallyltransferase activity; IEA:UniProtKB-EC.
DR CDD; cd13960; PT_UbiA_HPT1; 1.
DR Gene3D; 1.10.357.140; -; 1.
DR InterPro; IPR044502; AtHST-like.
DR InterPro; IPR000537; UbiA_prenyltransferase.
DR InterPro; IPR044878; UbiA_sf.
DR Pfam; PF01040; UbiA; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Magnesium; Membrane; Plastid; Reference proteome; Transferase;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..44
FT /note="Chloroplast"
FT /evidence="ECO:0000305"
FT CHAIN 45..409
FT /note="Glycinol 4-dimethylallyltransferase"
FT /id="PRO_0000418898"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 409 AA; 46012 MW; 20DA15CF484723E0 CRC64;
MDWGLAISSH PKPYSVTTGG NLWRSKHTTK NIYFASSWIS KASRHKRETQ IEHNVLRFQQ
PSLDHHYKCI RGGSTYQECN RKFVVKAISK QPLGFEAHAS NPKNILDSVK NVLSAFYWFS
YPYTMIGITL CAFSSSLLAV EKLSDISLSF LIGVLQGVLP QLFIEIYLCG VNQLYDLEID
KINKPHLPMA SGQFSFKTGV IISAAFLALS FGFTWITGSW PLICNLVVIA SSWTAYSIDV
PLLRWKRYPF VAAMCMISTW ALALPISYFH HMQTVVLKRP IGFPRSLGFL VAFMTFYSLG
LALSKDIPDV EGDKEHGIDS FAVRLGQKRA FWICVSFFEM AFGVGILAGA SCSHFWTKIF
TGMGNAVLAS ILWYQAKSVD LSDKASTGSF YMFIWKLLYA GFFLMALIR
