ID   G4P_BPFD                Reviewed;         426 AA.
AC   P03664;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Virion export protein;
DE   AltName: Full=Gene 4 protein;
DE            Short=G4P;
DE   Flags: Precursor;
GN   Name=IV;
OS   Enterobacteria phage fd (Bacteriophage fd).
OC   Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC   Tubulavirales; Inoviridae; Inovirus.
OX   NCBI_TaxID=2847073;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=478 / Heidelberg;
RX   PubMed=745987; DOI=10.1093/nar/5.12.4495;
RA   Beck E., Sommer R., Auerswald E.A., Kurz C., Zink B., Osterburg G.,
RA   Schaller H., Sugimoto K., Sugisaki H., Okamoto T., Takanami M.;
RT   "Nucleotide sequence of bacteriophage fd DNA.";
RL   Nucleic Acids Res. 5:4495-4503(1978).
CC   -!- FUNCTION: Acts in the assembly and extrusion of the bacteriophage by
CC       forming a channel across the host outer membrane. This channel is just
CC       large enough to allow a newly synthesized phage particle to pass
CC       through. Extrusion is a process of concomitant assembly and secretion
CC       and takes place at specific assembly sites where host inner and outer
CC       membranes are in close contacts (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimer. The channel is composed of 14 G4P subunits that
CC       confer a barrel-like structure. Interacts with G1P; this interaction
CC       results in a complex that spans the inner an outer host membranes (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass type I
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the inovirus G4P protein family. {ECO:0000305}.
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DR   EMBL; J02451; AAA32312.1; -; Genomic_DNA.
DR   PIR; A04268; Z4BPFD.
DR   RefSeq; YP_009111306.1; NC_025824.1.
DR   SMR; P03664; -.
DR   GeneID; 22475007; -.
DR   KEGG; vg:22475007; -.
DR   Proteomes; UP000001836; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   GO; GO:0099045; P:viral extrusion; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1370.120; -; 1.
DR   InterPro; IPR001775; GspD/PilQ.
DR   InterPro; IPR038591; NolW-like_sf.
DR   InterPro; IPR004846; T2SS/T3SS.
DR   InterPro; IPR004845; T2SS_GspD_CS.
DR   Pfam; PF00263; Secretin; 1.
DR   PRINTS; PR00811; BCTERIALGSPD.
DR   PROSITE; PS00875; T2SP_D; 1.
PE   3: Inferred from homology;
KW   Host membrane; Membrane; Signal; Transmembrane; Transmembrane helix;
KW   Viral extrusion; Viral release from host cell.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..426
FT                   /note="Virion export protein"
FT                   /id="PRO_0000209449"
FT   TRANSMEM        317..337
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   426 AA;  45823 MW;  5E54A766B843F7CB CRC64;
     MKLLNVINFV FLMFVSSSSF AQVIEMNNSP LRDFVTWYSK QTGESVIVSP DVKGTVTVYS
     SDVKPENLRN FFISVLRANN FDMVGSIPSI IQKYNPNSQD YIDELPSSDI QEYDDNSAPS
     GGFFVPQNDN VTQTFKINNV RAKDLIRVVE LFVKSNTSKS SNVLSVDGSN LLVVSAPKDI
     LDNLPQFLST VDLPTDQILI EGLIFEVQQG DALDFSFAAG SQRGTVAGGV NTDRLTSVLS
     SAGGSFGIFN GDVLGLSVRA LKTNSHSKIL SVPRILTLSG QKGSISVGQN VPFITGRVTG
     ESANVNNPFQ TVERQNVGIS MSVFPVAMAG GNIVLDITSK ADSLSSSTQA SDVITNQRSI
     ATTVNLRDGQ TLLLGGLTDY KNTSQDSGVP FLSKIPLIGL LFSSRSDSNE ESTLYVLVKA
     TIVRAL