ID   G5P_BPFD                Reviewed;          87 AA.
AC   P69542; P03669;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=DNA-Binding protein G5P;
DE            Short=G5P;
DE   AltName: Full=GPV;
DE   AltName: Full=Single-stranded DNA-binding protein;
GN   Name=V;
OS   Enterobacteria phage fd (Bacteriophage fd).
OC   Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC   Tubulavirales; Inoviridae; Inovirus.
OX   NCBI_TaxID=2847073;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=478 / Heidelberg;
RX   PubMed=745987; DOI=10.1093/nar/5.12.4495;
RA   Beck E., Sommer R., Auerswald E.A., Kurz C., Zink B., Osterburg G.,
RA   Schaller H., Sugimoto K., Sugisaki H., Okamoto T., Takanami M.;
RT   "Nucleotide sequence of bacteriophage fd DNA.";
RL   Nucleic Acids Res. 5:4495-4503(1978).
RN   [2]
RP   PROTEIN SEQUENCE.
RX   PubMed=4605211; DOI=10.1016/0014-5793(74)80246-2;
RA   Nakashima Y., Dunker A.K., Marvin D.A., Konigsberg W.;
RT   "The amino acid sequence of a DNA binding protein, the gene 5 product of fd
RT   filamentous bacteriophage.";
RL   FEBS Lett. 40:290-292(1974).
RN   [3]
RP   SEQUENCE REVISION.
RA   Nakashima Y., Dunker A.K., Marvin D.A., Konigsberg W.;
RL   FEBS Lett. 43:125-125(1974).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RX   PubMed=6264986; DOI=10.1016/s0006-3495(80)84931-9;
RA   McPherson A., Jurnak F., Wang A., Kolpak F., Rich A., Molineux I.,
RA   Fitzgerald P.;
RT   "The structure of a DNA unwinding protein and its complexes with
RT   oligodeoxynucleotides by X-ray diffraction.";
RL   Biophys. J. 32:155-173(1980).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RX   PubMed=6684697; DOI=10.1016/s0022-2836(83)80065-5;
RA   Brayer G.D., McPherson A.;
RT   "Refined structure of the gene 5 DNA binding protein from bacteriophage
RT   fd.";
RL   J. Mol. Biol. 169:565-596(1983).
CC   -!- FUNCTION: Binds to DNA in a highly cooperative manner without
CC       pronounced sequence specificity. During synthesis of the single-
CC       stranded (progeny) viral DNA, prevents the conversion into the double-
CC       stranded replicative form. G5P is displaced by the capsid protein G8P
CC       during phage assembly on the inner bacterial membrane (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the inovirus G5P protein family. {ECO:0000305}.
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DR   EMBL; J02451; AAA32305.1; -; Genomic_DNA.
DR   PIR; A04271; DDBPFD.
DR   RefSeq; YP_009111299.1; NC_025824.1.
DR   PDB; 2GN5; X-ray; 2.30 A; A=1-87.
DR   PDBsum; 2GN5; -.
DR   BMRB; P69542; -.
DR   SMR; P69542; -.
DR   GeneID; 22475000; -.
DR   KEGG; vg:22475000; -.
DR   EvolutionaryTrace; P69542; -.
DR   Proteomes; UP000001836; Genome.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039684; P:rolling circle single-stranded viral DNA replication; IDA:UniProtKB.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR003512; Phage_M13_G5P_DNA-bd.
DR   Pfam; PF02303; Phage_DNA_bind; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; DNA replication; DNA-binding.
FT   CHAIN           1..87
FT                   /note="DNA-Binding protein G5P"
FT                   /id="PRO_0000098194"
FT   SITE            16
FT                   /note="Involved in DNA binding"
FT   SITE            21
FT                   /note="Involved in DNA binding"
FT   SITE            26
FT                   /note="Involved in DNA binding"
FT   SITE            34
FT                   /note="Involved in DNA binding"
FT   SITE            41
FT                   /note="Involved in DNA binding, and in the dimer-dimer
FT                   interactions of the protein-ssDNA complex"
FT   SITE            46
FT                   /note="Involved in DNA binding"
FT   TURN            24..26
FT                   /evidence="ECO:0007829|PDB:2GN5"
FT   STRAND          39..41
FT                   /evidence="ECO:0007829|PDB:2GN5"
FT   STRAND          71..74
FT                   /evidence="ECO:0007829|PDB:2GN5"
SQ   SEQUENCE   87 AA;  9688 MW;  2FAD178DDC248CF0 CRC64;
     MIKVEIKPSQ AQFTTRSGVS RQGKPYSLNE QLCYVDLGNE YPVLVKITLD EGQPAYAPGL
     YTVHLSSFKV GQFGSLMIDR LRLVPAK