ID   G5P_BPIKE               Reviewed;          88 AA.
AC   P03670;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=DNA-Binding protein G5P;
DE            Short=G5P;
DE   AltName: Full=GPV;
DE   AltName: Full=Single-stranded DNA-binding protein;
GN   Name=V;
OS   Salmonella phage IKe (Bacteriophage IKe).
OC   Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC   Tubulavirales; Inoviridae; Lineavirus.
OX   NCBI_TaxID=10867;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6312049; DOI=10.1016/s0022-2836(83)80180-6;
RA   Peeters B.P.H., Konings R.N.H., Schoenmakers J.G.G.;
RT   "Characterization of the DNA binding protein encoded by the N-specific
RT   filamentous Escherichia coli phage IKe. Binding properties of the protein
RT   and nucleotide sequence of the gene.";
RL   J. Mol. Biol. 169:197-215(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3981635; DOI=10.1016/0022-2836(85)90322-5;
RA   Peeters B.P.H., Peters R.M., Schoenmakers J.G.G., Konings R.N.H.;
RT   "Nucleotide sequence and genetic organization of the genome of the N-
RT   specific filamentous bacteriophage IKe. Comparison with the genome of the
RT   F-specific filamentous phages M13, fd and f1.";
RL   J. Mol. Biol. 181:27-39(1985).
RN   [3]
RP   STRUCTURE BY NMR.
RX   PubMed=3308460; DOI=10.1111/j.1432-1033.1987.tb13373.x;
RA   de Jong E.A., Konings R.N.H., Harmsen B.J., Prinse C.W., Hilbers C.W.;
RT   "1H-NMR studies on the gene-5-encoded single-stranded DNA binding protein
RT   of the filamentous bacteriophage IKe. General spectral and structural
RT   features.";
RL   Eur. J. Biochem. 167:563-572(1987).
RN   [4]
RP   STRUCTURE BY NMR.
RX   PubMed=1734970; DOI=10.1021/bi00119a039;
RA   van Duynhoven J.P.M., Folkers P.J.M., Prinse C.W., Harmsen B.J.,
RA   Konings R.N.H., Hilbers C.W.;
RT   "Assignment of the 1H NMR spectrum and secondary structure elucidation of
RT   the single-stranded DNA binding protein encoded by the filamentous
RT   bacteriophage IKe.";
RL   Biochemistry 31:1254-1262(1992).
RN   [5]
RP   3D-STRUCTURE MODELING.
RX   PubMed=3270530; DOI=10.1080/07391102.1987.10507683;
RA   Brayer G.D.;
RT   "A preliminary structure for the DNA binding protein from bacteriophage
RT   IKe.";
RL   J. Biomol. Struct. Dyn. 4:859-868(1987).
CC   -!- FUNCTION: Binds to DNA in a highly cooperative manner without
CC       pronounced sequence specificity. During synthesis of the single-
CC       stranded (progeny) viral DNA, prevents the conversion into the double-
CC       stranded replicative form. G5P is displaced by the capsid protein G8P
CC       during phage assembly on the inner bacterial membrane (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the inovirus G5P protein family. {ECO:0000305}.
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DR   EMBL; X01914; CAA25988.1; -; Genomic_DNA.
DR   EMBL; X02139; CAA26069.1; -; Genomic_DNA.
DR   PIR; A04272; DDBPIK.
DR   RefSeq; NP_040572.1; NC_002014.1.
DR   BMRB; P03670; -.
DR   SMR; P03670; -.
DR   GeneID; 1260891; -.
DR   KEGG; vg:1260891; -.
DR   Proteomes; UP000000372; Genome.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR003512; Phage_M13_G5P_DNA-bd.
DR   Pfam; PF02303; Phage_DNA_bind; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
PE   1: Evidence at protein level;
KW   DNA replication; DNA-binding; Reference proteome.
FT   CHAIN           1..88
FT                   /note="DNA-Binding protein G5P"
FT                   /id="PRO_0000098199"
SQ   SEQUENCE   88 AA;  9813 MW;  07074FB935716CB9 CRC64;
     MLTVEIHDSQ VSVKERSGVS QKSGKPYTIR EQEAYIDLGG VYPALFNFNL EDGQQPYPAG
     KYRLHPASFK INNFGQVAVG RVLLESVK