ID   G5_VACCC                Reviewed;         434 AA.
AC   P21026;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Putative nuclease G5;
DE            EC=3.1.-.-;
GN   ORFNames=G5R;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Putative nuclease that seems to be required for double-strand
CC       break repair, homologous recombination, and production of full-length
CC       viral genomic DNA. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium ions per subunit. They probably participate in
CC       the reaction catalyzed by the enzyme. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=Present in the virion
CC       core. {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC   -!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. FEN1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; M35027; AAA48069.1; -; Genomic_DNA.
DR   PIR; A42512; A42512.
DR   PRIDE; P21026; -.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   InterPro; IPR007678; Poxvirus_G5.
DR   Pfam; PF04599; Pox_G5; 1.
PE   2: Evidence at transcript level;
KW   DNA damage; DNA repair; Early protein; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease; Reference proteome; Virion.
FT   CHAIN           1..434
FT                   /note="Putative nuclease G5"
FT                   /id="PRO_0000099531"
FT   BINDING         33
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         74
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         168
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         170
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         196
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         198
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   434 AA;  49873 MW;  D1DD4DC56A0AF5A0 CRC64;
     MGIKNLKSLL LENKSLTILD DNLYKVYNGI FVDTMSIYIA VANCVRNLEE LTTVFIKYVN
     GWVKKGGHVT LFIDRGSIKI KQDVRDKRRK YSKLTKDRKM LELEKCTSEI QNVTGFMEEE
     IKAEMQLKID KLTFQIYLSD SDNIKISLNE ILTHFNNNEN VTLFYCDERD AEFVMCLEAK
     THFSTTGEWP LIISTDQDTM LFASTDNHPK MIKNLTQLFK FVPSAEDNYL AKLTALVNGC
     DFFPGLYGAS ITPNNLNKIQ LFSDFTIDNI VTSLAIKNYY RKTNSTVDVR NIVTFINDYA
     NLDDVYSYVP PCQCTVQEFI FSALDEKWNN FKSSYLETVP LPCQLMYALE PRKEIDVSEV
     KTLSSYIDFE NTKSDIDVIK SISSIFGYSN ENCNTIVFGI YKDNLLLSIN NSFYFNDSLL
     ITNTKSDNII NIGY