ID   G5_VAR67                Reviewed;         434 AA.
AC   P0DSS5; P32995;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   03-AUG-2022, entry version 7.
DE   RecName: Full=Putative nuclease G5;
DE            EC=3.1.-.-;
GN   ORFNames=G5R;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8109158; DOI=10.1016/0168-1702(93)90093-3;
RA   Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V.,
RA   Sandakhchiev L.S.;
RT   "Analysis of the nucleotide sequence of a 43 kbp segment of the genome of
RT   variola virus India-1967 strain.";
RL   Virus Res. 30:239-258(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8383392; DOI=10.1016/0168-1702(93)90110-9;
RA   Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S.,
RA   Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V.,
RA   Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G.,
RA   Sandakhchiev L.S.;
RT   "Nucleotide sequence analysis of variola virus HindIII M, L, I genome
RT   fragments.";
RL   Virus Res. 27:25-35(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Putative nuclease that seems to be required for double-strand
CC       break repair, homologous recombination, and production of full-length
CC       viral genomic DNA. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium ions per subunit. They probably participate in
CC       the reaction catalyzed by the enzyme. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=Present in the virion
CC       core. {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC   -!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. FEN1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; X67119; CAA47567.1; -; Genomic_DNA.
DR   EMBL; X69198; CAA49008.1; -; Genomic_DNA.
DR   PIR; S33082; S33082.
DR   RefSeq; NP_042111.1; NC_001611.1.
DR   GeneID; 1486433; -.
DR   KEGG; vg:1486433; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   InterPro; IPR007678; Poxvirus_G5.
DR   Pfam; PF04599; Pox_G5; 1.
PE   2: Evidence at transcript level;
KW   DNA damage; DNA repair; Early protein; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease; Reference proteome; Virion.
FT   CHAIN           1..434
FT                   /note="Putative nuclease G5"
FT                   /id="PRO_0000099532"
FT   BINDING         33
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         74
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         168
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         170
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         196
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         198
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   434 AA;  49884 MW;  DD5214A9C9810ACD CRC64;
     MGIKNLKSLL LENKSLTILD DNLYKVYNGI FVDTMSIYIA VANCVRNLEE LTTVFIKYVN
     GWVKKGGHVT LFIDRGSIKI KQNVRDKRRK YSKSTKDRKM LELEKCTSKI QNVTGFMEEE
     IKAEIQLKID KLTFQIYLSD SDNIKISLNE ILTHFNNNEN VTLFYCDERD AEFVMCLEAK
     TYFFTTGEWP LIISTDQDTM LFASVDNHPK MIKNLTQLFK FVPSAEDNYL AKLTALVNGC
     DFFPGLYGAS ITPTNLNKIQ LFSDFTINNI VTSLAIKNYY RKTNSTVDVR NIVTFINDYA
     NLDDVYSYIP PCQCTVQEFI FSALDEKWND FKSSYLETVP LPCQLMYALE PRKEIDVSEV
     KTLSSYIDFE NTKSDIDVIK SISSIFGYSN ENCNTIVFGI YKDNLLLSIN SSFYFNNSLL
     ITNTKSDNII NIGY