G6B_RAT
ID G6B_RAT Reviewed; 232 AA.
AC Q6MG59;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Megakaryocyte and platelet inhibitory receptor G6b {ECO:0000312|RGD:1303269};
DE AltName: Full=Protein G6b {ECO:0000305};
DE Flags: Precursor;
GN Name=Mpig6b {ECO:0000312|RGD:1303269};
GN Synonyms=G6b {ECO:0000312|RGD:1303269};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15060004; DOI=10.1101/gr.1987704;
RA Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H.,
RA Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.;
RT "The genomic sequence and comparative analysis of the rat major
RT histocompatibility complex.";
RL Genome Res. 14:631-639(2004).
CC -!- FUNCTION: Inhibitory receptor that acts as a critical regulator of
CC hematopoietic lineage differentiation, megakaryocyte function and
CC platelet production. Inhibits platelet aggregation and activation by
CC agonists such as ADP and collagen-related peptide. This regulation of
CC megakaryocate function as well as platelet production ann activation is
CC done through the inhibition (via the 2 ITIM motifs) of the receptors
CC CLEC1B and GP6:FcRgamma signaling. Appears to operate in a calcium-
CC independent manner. {ECO:0000250|UniProtKB:D7PDD4,
CC ECO:0000250|UniProtKB:O95866}.
CC -!- SUBUNIT: Interacts (via ITIM motif) with PTPN6 and PTPN11. Binds to
CC heparin. {ECO:0000250|UniProtKB:D7PDD4, ECO:0000250|UniProtKB:O95866}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:O95866};
CC Single-pass type I membrane protein {ECO:0000250|UniProtKB:O95866}.
CC -!- DOMAIN: Contains both a transmembrane region and 2 copies of a
CC cytoplasmic motif that is referred to as the immunoreceptor tyrosine-
CC based inhibitor motif (ITIM). This motif is involved in modulation of
CC cellular responses. The phosphorylated ITIM motif can bind the SH2
CC domain of several SH2-containing phosphatases. The 2 ITIM motifs of
CC isoform B are required for the inhibition of CLEC1B and GP6:FCER1G
CC signaling and platelet activation. {ECO:0000250|UniProtKB:D7PDD4}.
CC -!- PTM: N-glycosylated. {ECO:0000250|UniProtKB:D7PDD4,
CC ECO:0000250|UniProtKB:O95866}.
CC -!- PTM: May be O-glycosylated. {ECO:0000250|UniProtKB:O95866}.
CC -!- PTM: Phosphorylated. {ECO:0000250|UniProtKB:D7PDD4}.
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DR EMBL; BX883045; CAE83987.1; -; Genomic_DNA.
DR RefSeq; NP_001003976.1; NM_001003976.1.
DR AlphaFoldDB; Q6MG59; -.
DR SMR; Q6MG59; -.
DR STRING; 10116.ENSRNOP00000068435; -.
DR GlyGen; Q6MG59; 2 sites.
DR PaxDb; Q6MG59; -.
DR Ensembl; ENSRNOT00000037191; ENSRNOP00000033088; ENSRNOG00000026936.
DR GeneID; 406865; -.
DR KEGG; rno:406865; -.
DR UCSC; RGD:1303269; rat.
DR CTD; 80739; -.
DR RGD; 1303269; Mpig6b.
DR eggNOG; ENOG502TEAD; Eukaryota.
DR GeneTree; ENSGT00390000017793; -.
DR InParanoid; Q6MG59; -.
DR PhylomeDB; Q6MG59; -.
DR TreeFam; TF337394; -.
DR Reactome; R-RNO-114604; GPVI-mediated activation cascade.
DR PRO; PR:Q6MG59; -.
DR Proteomes; UP000002494; Chromosome 20.
DR Bgee; ENSRNOG00000026936; Expressed in ovary and 14 other tissues.
DR ExpressionAtlas; Q6MG59; baseline.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008201; F:heparin binding; IEA:UniProtKB-KW.
DR GO; GO:0007596; P:blood coagulation; ISS:UniProtKB.
DR GO; GO:0030218; P:erythrocyte differentiation; ISS:UniProtKB.
DR GO; GO:0007229; P:integrin-mediated signaling pathway; ISS:UniProtKB.
DR GO; GO:0035855; P:megakaryocyte development; ISS:UniProtKB.
DR GO; GO:0030219; P:megakaryocyte differentiation; ISS:UniProtKB.
DR GO; GO:0009968; P:negative regulation of signal transduction; ISS:UniProtKB.
DR GO; GO:0030220; P:platelet formation; ISS:UniProtKB.
DR InterPro; IPR028070; G6B.
DR PANTHER; PTHR37347; PTHR37347; 1.
DR Pfam; PF15096; G6B; 1.
PE 3: Inferred from homology;
KW Cell membrane; Glycoprotein; Heparin-binding; Membrane; Phosphoprotein;
KW Receptor; Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..17
FT /evidence="ECO:0000250"
FT CHAIN 18..232
FT /note="Megakaryocyte and platelet inhibitory receptor G6b"
FT /id="PRO_0000021313"
FT TOPO_DOM 18..140
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..232
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOTIF 200..205
FT /note="ITIM motif"
FT /evidence="ECO:0000250|UniProtKB:O95866"
FT MOTIF 226..231
FT /note="ITIM motif"
FT /evidence="ECO:0000250|UniProtKB:O95866"
FT MOD_RES 202
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O95866"
FT CARBOHYD 32
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 112
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 232 AA; 25083 MW; 728345F61CFD085A CRC64;
MALVLQLLPL LLSKVQGNPE VSLEGNPGDR VNLSCIGVSD PTRWAWAPSF PACKGLSKGR
RPILWASSSG TPTVLQHFSG RLRSLDTGIK RLELLLSAGD SGTFFCKGRQ ENESRTVIQV
LGDKAGCRPS GSTHGSEYSK VLIPLLGFGL VLGLGALGLV WWRRSCVPPS HIAPVINAEP
QRPLEQDSKI SGHLDQEPNL HYADLDHSVL RRHRRMSALV PGDASTVYAV VV
