ID   ALG14_YARLI             Reviewed;         216 AA.
AC   Q6CF02;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=UDP-N-acetylglucosamine transferase subunit ALG14;
DE   AltName: Full=Asparagine-linked glycosylation protein 14;
GN   Name=ALG14; OrderedLocusNames=YALI0B11440g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Involved in protein N-glycosylation. Essential for the second
CC       step of the dolichol-linked oligosaccharide pathway (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with ALG13 to form a functional enzyme.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:P38242}; Single-pass membrane protein
CC       {ECO:0000255}. Nucleus membrane {ECO:0000250|UniProtKB:P38242}; Single-
CC       pass membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the ALG14 family. {ECO:0000305}.
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DR   EMBL; CR382128; CAG83007.1; -; Genomic_DNA.
DR   RefSeq; XP_500760.1; XM_500760.1.
DR   AlphaFoldDB; Q6CF02; -.
DR   SMR; Q6CF02; -.
DR   STRING; 4952.CAG83007; -.
DR   EnsemblFungi; CAG83007; CAG83007; YALI0_B11440g.
DR   GeneID; 2907489; -.
DR   KEGG; yli:YALI0B11440g; -.
DR   VEuPathDB; FungiDB:YALI0_B11440g; -.
DR   HOGENOM; CLU_064541_0_1_1; -.
DR   InParanoid; Q6CF02; -.
DR   OMA; GPGTCCI; -.
DR   Proteomes; UP000001300; Chromosome B.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043541; C:UDP-N-acetylglucosamine transferase complex; IBA:GO_Central.
DR   GO; GO:0006488; P:dolichol-linked oligosaccharide biosynthetic process; IBA:GO_Central.
DR   InterPro; IPR013969; Oligosacch_biosynth_Alg14.
DR   PANTHER; PTHR12154; PTHR12154; 1.
DR   Pfam; PF08660; Alg14; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Membrane; Nucleus; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..216
FT                   /note="UDP-N-acetylglucosamine transferase subunit ALG14"
FT                   /id="PRO_0000123817"
FT   TOPO_DOM        1..4
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250|UniProtKB:P38242"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        26..216
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P38242"
SQ   SEQUENCE   216 AA;  24108 MW;  DA57CF2BD86DE8C7 CRC64;
     MVTTILIAAS AILVLLLLRL LFVLPASNRF GFLYRPKHSN PKLMVMMGSG GHTGEMLRML
     KTLKLQSYAK RVYVSSSGDV DSLEKVKVLE STTKTDIKTM VLENIPRARK VGQSYPSSVI
     TSAVSFAVAV KLVHKHKPHV IVCNGPATCV MLCYAAFLLR FMALIDTRII YVESLARVNR
     LSLSGLILLP FCDRFLVQWP QLAEKYPRAE YHGILV