G6PD_STRPN
ID G6PD_STRPN Reviewed; 495 AA.
AC O54537;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Glucose-6-phosphate 1-dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00966};
DE Short=G6PD {ECO:0000255|HAMAP-Rule:MF_00966};
DE EC=1.1.1.49 {ECO:0000255|HAMAP-Rule:MF_00966};
GN Name=zwf {ECO:0000255|HAMAP-Rule:MF_00966}; OrderedLocusNames=SP_1243;
OS Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=170187;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-334 / TIGR4;
RX PubMed=11463916; DOI=10.1126/science.1061217;
RA Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT "Complete genome sequence of a virulent isolate of Streptococcus
RT pneumoniae.";
RL Science 293:498-506(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 281-433.
RC STRAIN=ATCC 6323;
RX PubMed=9466257; DOI=10.1046/j.1365-2958.1998.00658.x;
RA Coffey T.J., Enright M.C., Daniels M., Morona J.K., Morona R.,
RA Hryniewicz W., Paton J.C., Spratt B.G.;
RT "Recombinational exchanges at the capsular polysaccharide biosynthetic
RT locus lead to frequent serotype changes among natural isolates of
RT Streptococcus pneumoniae.";
RL Mol. Microbiol. 27:73-83(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 281-433.
RX PubMed=9533725; DOI=10.1089/mdr.1998.4.51;
RA Coffey T.J., Enright M.C., Daniels M., Wilkinson P., Berron S., Fenoll A.,
RA Spratt B.G.;
RT "Serotype 19A variants of the Spanish serotype 23F multiresistant clone of
RT Streptococcus pneumoniae.";
RL Microb. Drug Resist. 4:51-55(1998).
CC -!- FUNCTION: Catalyzes the oxidation of glucose 6-phosphate to 6-
CC phosphogluconolactone. {ECO:0000255|HAMAP-Rule:MF_00966}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-
CC lactone + H(+) + NADPH; Xref=Rhea:RHEA:15841, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:57955, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:61548; EC=1.1.1.49; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00966};
CC -!- PATHWAY: Carbohydrate degradation; pentose phosphate pathway; D-
CC ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step
CC 1/3. {ECO:0000255|HAMAP-Rule:MF_00966}.
CC -!- SIMILARITY: Belongs to the glucose-6-phosphate dehydrogenase family.
CC {ECO:0000255|HAMAP-Rule:MF_00966}.
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DR EMBL; AE005672; AAK75348.1; -; Genomic_DNA.
DR EMBL; Z99802; CAB16927.1; -; Genomic_DNA.
DR EMBL; Z99865; CAB16990.1; -; Genomic_DNA.
DR PIR; C95144; C95144.
DR RefSeq; WP_000096161.1; NZ_AKVY01000001.1.
DR AlphaFoldDB; O54537; -.
DR SMR; O54537; -.
DR STRING; 170187.SP_1243; -.
DR PRIDE; O54537; -.
DR EnsemblBacteria; AAK75348; AAK75348; SP_1243.
DR KEGG; spn:SP_1243; -.
DR eggNOG; COG0364; Bacteria.
DR OMA; VEICVYE; -.
DR PhylomeDB; O54537; -.
DR BioCyc; SPNE170187:G1FZB-1257-MON; -.
DR UniPathway; UPA00115; UER00408.
DR Proteomes; UP000000585; Chromosome.
DR GO; GO:0004345; F:glucose-6-phosphate dehydrogenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0050661; F:NADP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006098; P:pentose-phosphate shunt; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00966; G6PD; 1.
DR InterPro; IPR001282; G6P_DH.
DR InterPro; IPR019796; G6P_DH_AS.
DR InterPro; IPR022675; G6P_DH_C.
DR InterPro; IPR022674; G6P_DH_NAD-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR23429; PTHR23429; 1.
DR Pfam; PF02781; G6PD_C; 1.
DR Pfam; PF00479; G6PD_N; 1.
DR PIRSF; PIRSF000110; G6PD; 1.
DR PRINTS; PR00079; G6PDHDRGNASE.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR00871; zwf; 1.
DR PROSITE; PS00069; G6P_DEHYDROGENASE; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glucose metabolism; NADP; Oxidoreductase.
FT CHAIN 1..495
FT /note="Glucose-6-phosphate 1-dehydrogenase"
FT /id="PRO_0000068133"
FT ACT_SITE 239
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00966"
FT BINDING 11..18
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00966"
FT BINDING 45
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00966"
FT BINDING 84..85
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00966"
FT BINDING 147
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00966"
FT BINDING 177
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00966"
FT BINDING 181
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00966"
FT BINDING 215
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00966"
FT BINDING 234
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00966"
FT BINDING 339
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00966"
FT BINDING 344
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00966"
FT CONFLICT 348
FT /note="V -> I (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 495 AA; 56790 MW; 99093E9C8929FFAF CRC64;
MSSKVIVTIF GASGDLAKRK LYPSLFRLYQ SGNLSKHFAV IGTARRPWSK EYFESVVVES
ILDLADSTEQ AQEFASHFYY QSHDVNDSEH YIALRQLQAE LNEKYQAEHN KLFFLSMAPQ
FFGTIAKHLK SENIVDGKGF ERLIVEKPFG TDYATASKLN DELLATFDEE QIFRIDHYLG
KEMIQSIFAV RFANLIFENV WNKDFIDNVQ ITFAERLGVE ERGGYYDQSG ALRDMVQNHT
LQLLSLLAMD KPASFTKDEI RAEKIKVFKN LYHPTDEELK EHFIRGQYRS GKIDGMKYIS
YRSEPNVNPE STTETFTSGA FFVDSDRFRG VPFFFRTGKR LTEKGTHVNI VFKQMDSIFG
EPLAPNILTI YIQPTEGFSL SLNGKQVGEE FNLAPNSLDY RTDATATGAS PEPYEKLIYD
VLNNNSTNFS HWDEVCASWK LIDRIEKLWA ENGAPLHDYK AGSMGPQASF DLLEKFGAKW
TWQPDITYRQ DGRLE
