G6PI_BACTN
ID G6PI_BACTN Reviewed; 445 AA.
AC Q8A5W2;
DT 23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 23-MAY-2003, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Glucose-6-phosphate isomerase {ECO:0000255|HAMAP-Rule:MF_00473};
DE Short=GPI {ECO:0000255|HAMAP-Rule:MF_00473};
DE EC=5.3.1.9 {ECO:0000255|HAMAP-Rule:MF_00473};
DE AltName: Full=Phosphoglucose isomerase {ECO:0000255|HAMAP-Rule:MF_00473};
DE Short=PGI {ECO:0000255|HAMAP-Rule:MF_00473};
DE AltName: Full=Phosphohexose isomerase {ECO:0000255|HAMAP-Rule:MF_00473};
DE Short=PHI {ECO:0000255|HAMAP-Rule:MF_00473};
GN Name=pgi {ECO:0000255|HAMAP-Rule:MF_00473}; OrderedLocusNames=BT_2124;
OS Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 /
OS CCUG 10774 / NCTC 10582 / VPI-5482 / E50).
OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=226186;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC VPI-5482 / E50;
RX PubMed=12663928; DOI=10.1126/science.1080029;
RA Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C.,
RA Hooper L.V., Gordon J.I.;
RT "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis.";
RL Science 299:2074-2076(2003).
CC -!- FUNCTION: Catalyzes the reversible isomerization of glucose-6-phosphate
CC to fructose-6-phosphate. {ECO:0000255|HAMAP-Rule:MF_00473}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate;
CC Xref=Rhea:RHEA:11816, ChEBI:CHEBI:57634, ChEBI:CHEBI:58225;
CC EC=5.3.1.9; Evidence={ECO:0000255|HAMAP-Rule:MF_00473};
CC -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC {ECO:0000255|HAMAP-Rule:MF_00473}.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 2/4.
CC {ECO:0000255|HAMAP-Rule:MF_00473}.
CC -!- INTERACTION:
CC Q8A5W2; Q8A2Y3: BT_3172; NbExp=3; IntAct=EBI-15584961, EBI-15584902;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00473}.
CC -!- SIMILARITY: Belongs to the GPI family. {ECO:0000255|HAMAP-
CC Rule:MF_00473}.
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DR EMBL; AE015928; AAO77231.1; -; Genomic_DNA.
DR RefSeq; NP_811037.1; NC_004663.1.
DR RefSeq; WP_008759774.1; NC_004663.1.
DR AlphaFoldDB; Q8A5W2; -.
DR SMR; Q8A5W2; -.
DR DIP; DIP-61177N; -.
DR IntAct; Q8A5W2; 1.
DR STRING; 226186.BT_2124; -.
DR PaxDb; Q8A5W2; -.
DR PRIDE; Q8A5W2; -.
DR EnsemblBacteria; AAO77231; AAO77231; BT_2124.
DR GeneID; 60928112; -.
DR KEGG; bth:BT_2124; -.
DR PATRIC; fig|226186.12.peg.2186; -.
DR eggNOG; COG0166; Bacteria.
DR HOGENOM; CLU_037303_0_1_10; -.
DR InParanoid; Q8A5W2; -.
DR OMA; NNIGEDY; -.
DR UniPathway; UPA00109; UER00181.
DR UniPathway; UPA00138; -.
DR Proteomes; UP000001414; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR GO; GO:0004347; F:glucose-6-phosphate isomerase activity; IBA:GO_Central.
DR GO; GO:0048029; F:monosaccharide binding; IBA:GO_Central.
DR GO; GO:0006094; P:gluconeogenesis; IBA:GO_Central.
DR GO; GO:0051156; P:glucose 6-phosphate metabolic process; IBA:GO_Central.
DR GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR CDD; cd05015; SIS_PGI_1; 1.
DR CDD; cd05016; SIS_PGI_2; 1.
DR HAMAP; MF_00473; G6P_isomerase; 1.
DR InterPro; IPR001672; G6P_Isomerase.
DR InterPro; IPR018189; Phosphoglucose_isomerase_CS.
DR InterPro; IPR046348; SIS_dom_sf.
DR InterPro; IPR035476; SIS_PGI_1.
DR InterPro; IPR035482; SIS_PGI_2.
DR PANTHER; PTHR11469; PTHR11469; 1.
DR Pfam; PF00342; PGI; 1.
DR PRINTS; PR00662; G6PISOMERASE.
DR SUPFAM; SSF53697; SSF53697; 1.
DR PROSITE; PS00765; P_GLUCOSE_ISOMERASE_1; 1.
DR PROSITE; PS00174; P_GLUCOSE_ISOMERASE_2; 1.
DR PROSITE; PS51463; P_GLUCOSE_ISOMERASE_3; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Gluconeogenesis; Glycolysis; Isomerase; Reference proteome.
FT CHAIN 1..445
FT /note="Glucose-6-phosphate isomerase"
FT /id="PRO_0000180597"
FT ACT_SITE 287
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00473"
FT ACT_SITE 308
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00473"
FT ACT_SITE 422
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00473"
SQ SEQUENCE 445 AA; 48891 MW; C80209EA1A7E63AA CRC64;
MISLNIEKTF GFISKEKVSA YEAEVKAAQE MLEKGTGEGN DFLGWLHLPS SISKEHLADL
NATAKVLRDN CEVVIVAGIG GSYLGARAVI EALSNSFTWL QDKKTAPVMI YAGHNISEDY
LYELTEYLKD KKFGVINISK SGTTTETALA FRLLKKQCED QRGKETAKKV IVAVTDAKKG
AARVTADKEG YKTFIIPDNV GGRFSVLTPV GLLPIAVAGF DIDKLVAGAA DMEKACGSDV
PFAENPAAIY AATRNELYRQ GKKIEILVNF CPKLHYVSEW WKQLYGESEG KDNKGIFPAS
VDFSTDLHSM GQWIQEGERS IFETVISLDK VDHKLEVPFD EANLDGLNFL AGKRVDEVNK
MAELGTQLAH VDGGVPNMRI VLPELSEYNI GGLLYFFEKA CGISGYLLGV NPFNQPGVEA
YKKNMFALLN KPGYEEESKA IQAKL
