G6PI_DROME
ID G6PI_DROME Reviewed; 558 AA.
AC P52029; A4UZ93; Q0E9F0; Q27868; Q27870; Q9V4Z8;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Glucose-6-phosphate isomerase;
DE Short=GPI;
DE EC=5.3.1.9;
DE AltName: Full=Phosphoglucose isomerase;
DE Short=PGI;
DE AltName: Full=Phosphohexose isomerase;
DE Short=PHI;
GN Name=Pgi; ORFNames=CG8251;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS TYR-184; ILE-329 AND
RP ALA-471.
RC STRAIN=AF-F, AF-S, F1-1S, F1-F, FL-2S, FR-F, FR-S, JA-F, JA-S, LW15, LW8,
RC MW13, MW17, MW18, WA-F, WA-S, ZW20, ZW29, ZW34, ZW53, and ZW56;
RA McDonald J.H., Kreitman M.E.;
RL Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate;
CC Xref=Rhea:RHEA:11816, ChEBI:CHEBI:57634, ChEBI:CHEBI:58225;
CC EC=5.3.1.9;
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 2/4.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GPI family. {ECO:0000305}.
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DR EMBL; L27539; AAA28789.1; -; Genomic_DNA.
DR EMBL; L27540; AAA28790.1; -; Genomic_DNA.
DR EMBL; L27541; AAA28791.1; -; Genomic_DNA.
DR EMBL; L27542; AAA28792.1; -; Genomic_DNA.
DR EMBL; L27543; AAA28793.1; -; Genomic_DNA.
DR EMBL; L27544; AAA28794.1; -; Genomic_DNA.
DR EMBL; L27545; AAA28795.1; -; Genomic_DNA.
DR EMBL; L27546; AAA28796.1; -; Genomic_DNA.
DR EMBL; L27553; AAA28803.1; -; Genomic_DNA.
DR EMBL; L27554; AAA28804.1; -; Genomic_DNA.
DR EMBL; L27555; AAA28805.1; -; Genomic_DNA.
DR EMBL; U20566; AAA63671.1; -; Genomic_DNA.
DR EMBL; U20567; AAA63672.1; -; Genomic_DNA.
DR EMBL; U20568; AAA63673.1; -; Genomic_DNA.
DR EMBL; U20569; AAA63674.1; -; Genomic_DNA.
DR EMBL; U20570; AAA63675.1; -; Genomic_DNA.
DR EMBL; U20571; AAA63676.1; -; Genomic_DNA.
DR EMBL; U20572; AAA63677.1; -; Genomic_DNA.
DR EMBL; U20573; AAA63678.1; -; Genomic_DNA.
DR EMBL; U20574; AAA63679.1; -; Genomic_DNA.
DR EMBL; U20575; AAA63680.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF59025.1; -; Genomic_DNA.
DR EMBL; AE013599; AAM71091.1; -; Genomic_DNA.
DR EMBL; AY051468; AAK92892.1; -; mRNA.
DR RefSeq; NP_523663.1; NM_078939.3.
DR RefSeq; NP_724723.1; NM_165635.2.
DR RefSeq; NP_724724.1; NM_165636.2.
DR AlphaFoldDB; P52029; -.
DR SMR; P52029; -.
DR BioGRID; 61725; 6.
DR DIP; DIP-17637N; -.
DR IntAct; P52029; 5.
DR STRING; 7227.FBpp0087760; -.
DR PaxDb; P52029; -.
DR PRIDE; P52029; -.
DR DNASU; 35886; -.
DR EnsemblMetazoa; FBtr0088679; FBpp0087760; FBgn0003074.
DR EnsemblMetazoa; FBtr0088680; FBpp0087761; FBgn0003074.
DR EnsemblMetazoa; FBtr0088681; FBpp0087762; FBgn0003074.
DR GeneID; 35886; -.
DR KEGG; dme:Dmel_CG8251; -.
DR CTD; 35886; -.
DR FlyBase; FBgn0003074; Pgi.
DR VEuPathDB; VectorBase:FBgn0003074; -.
DR eggNOG; KOG2446; Eukaryota.
DR GeneTree; ENSGT00390000000707; -.
DR HOGENOM; CLU_017947_3_1_1; -.
DR InParanoid; P52029; -.
DR OMA; IGVWYIN; -.
DR OrthoDB; 446616at2759; -.
DR PhylomeDB; P52029; -.
DR Reactome; R-DME-5628897; TP53 Regulates Metabolic Genes.
DR Reactome; R-DME-6798695; Neutrophil degranulation.
DR Reactome; R-DME-70171; Glycolysis.
DR Reactome; R-DME-70263; Gluconeogenesis.
DR SignaLink; P52029; -.
DR UniPathway; UPA00109; UER00181.
DR BioGRID-ORCS; 35886; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35886; -.
DR PRO; PR:P52029; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0003074; Expressed in adult hindgut (Drosophila) and 29 other tissues.
DR Genevisible; P52029; DM.
DR GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR GO; GO:0004347; F:glucose-6-phosphate isomerase activity; IDA:FlyBase.
DR GO; GO:0048029; F:monosaccharide binding; IBA:GO_Central.
DR GO; GO:0006094; P:gluconeogenesis; IBA:GO_Central.
DR GO; GO:0051156; P:glucose 6-phosphate metabolic process; IBA:GO_Central.
DR GO; GO:0042593; P:glucose homeostasis; IMP:FlyBase.
DR GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR CDD; cd05015; SIS_PGI_1; 1.
DR CDD; cd05016; SIS_PGI_2; 1.
DR Gene3D; 1.10.1390.10; -; 1.
DR HAMAP; MF_00473; G6P_isomerase; 1.
DR InterPro; IPR001672; G6P_Isomerase.
DR InterPro; IPR023096; G6P_Isomerase_C.
DR InterPro; IPR018189; Phosphoglucose_isomerase_CS.
DR InterPro; IPR046348; SIS_dom_sf.
DR InterPro; IPR035476; SIS_PGI_1.
DR InterPro; IPR035482; SIS_PGI_2.
DR PANTHER; PTHR11469; PTHR11469; 1.
DR Pfam; PF00342; PGI; 1.
DR PRINTS; PR00662; G6PISOMERASE.
DR SUPFAM; SSF53697; SSF53697; 1.
DR PROSITE; PS00765; P_GLUCOSE_ISOMERASE_1; 1.
DR PROSITE; PS00174; P_GLUCOSE_ISOMERASE_2; 1.
DR PROSITE; PS51463; P_GLUCOSE_ISOMERASE_3; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Gluconeogenesis; Glycolysis; Isomerase; Reference proteome.
FT CHAIN 1..558
FT /note="Glucose-6-phosphate isomerase"
FT /id="PRO_0000180543"
FT ACT_SITE 362
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 393
FT /evidence="ECO:0000250"
FT ACT_SITE 523
FT /evidence="ECO:0000250"
FT VARIANT 184
FT /note="H -> Y (in strain: LW15)"
FT /evidence="ECO:0000269|Ref.1"
FT VARIANT 329
FT /note="V -> I (in strain: AF-F, F1-F, F1-1S, JA-F, LW8,
FT MW18 and ZW20)"
FT /evidence="ECO:0000269|Ref.1"
FT VARIANT 471
FT /note="V -> A (in strain: JA-S)"
FT /evidence="ECO:0000269|Ref.1"
SQ SEQUENCE 558 AA; 62339 MW; 8DD54FCFCCBD1488 CRC64;
MAGPLPPLNQ EAAFQKLQEY YDSKGKDLNI KDLFVKDSKR FSKYSLRLHT QNDGEILLDY
SKNRINDEVW DLLLTLAKVR RVNAARDAMF SGQHINITEN RAVLHTALRN RGTDPVLVDD
KDVMPDVRAE LAHMKEFTNM VISGVWRGCT GKQITDVVNI GIGGSDLGPL MVTEALKPYG
KGLHSHFVSN IDGTHLAEVL KKVNYETTLF IVASKTFTTQ ETITNATSAK TWLLEHSKEP
ESVAKHFVAL STNKEKVTEF GIDSTNMFGF WDWVGGRYSL WSAIGLSICL SIGFENFEQL
LDGAHFMDNH FKTTPFEKNA PVILALLGVW YSNFFKAETH ALLPYDQYLH RFAAYFQQGD
MESNGKFVSK SGKPVKYSTG PIVWGEPGTN GQHAFYQLIH QGTRLIPCDF IAPAQTHNPI
AGGKHHKILL SNFLAQTEAL MAGKTVDEAR TELSKAGLCG NELDNLLPHK VFVGNRPTNS
IVVKKVSPFT LGALIALYEH KIFVQGIIWD INSFDQWGVE LGKQLAKAIE PELDHCNEVS
THDSSTNGLI NFIKANWK
