ID   G6PI_DROSI              Reviewed;         558 AA.
AC   P52030; Q27867;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Glucose-6-phosphate isomerase;
DE            Short=GPI;
DE            EC=5.3.1.9;
DE   AltName: Full=Phosphoglucose isomerase;
DE            Short=PGI;
DE   AltName: Full=Phosphohexose isomerase;
DE            Short=PHI;
GN   Name=Pgi;
OS   Drosophila simulans (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7240;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Various strains;
RA   McDonald J.H., Kreitman M.E.;
RL   Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate;
CC         Xref=Rhea:RHEA:11816, ChEBI:CHEBI:57634, ChEBI:CHEBI:58225;
CC         EC=5.3.1.9;
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 2/4.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GPI family. {ECO:0000305}.
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DR   EMBL; L27547; AAA28797.1; -; Genomic_DNA.
DR   EMBL; L27548; AAA28798.1; -; Genomic_DNA.
DR   EMBL; L27549; AAA28799.1; -; Genomic_DNA.
DR   EMBL; L27550; AAA28800.1; -; Genomic_DNA.
DR   EMBL; L27551; AAA28801.1; -; Genomic_DNA.
DR   EMBL; L27552; AAA28802.1; -; Genomic_DNA.
DR   EMBL; U20556; AAA63663.1; -; Genomic_DNA.
DR   EMBL; U20557; AAA63664.1; -; Genomic_DNA.
DR   EMBL; U20558; AAA63665.1; -; Genomic_DNA.
DR   EMBL; U20559; AAA63666.1; -; Genomic_DNA.
DR   EMBL; U20560; AAA63667.1; -; Genomic_DNA.
DR   EMBL; U20561; AAA63668.1; -; Genomic_DNA.
DR   EMBL; U20564; AAA63669.1; -; Genomic_DNA.
DR   EMBL; U20565; AAA63670.1; -; Genomic_DNA.
DR   RefSeq; XP_016026575.1; XM_016184269.1.
DR   RefSeq; XP_016026576.1; XM_016184270.1.
DR   AlphaFoldDB; P52030; -.
DR   SMR; P52030; -.
DR   GeneID; 27208705; -.
DR   UniPathway; UPA00109; UER00181.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR   GO; GO:0004347; F:glucose-6-phosphate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd05015; SIS_PGI_1; 1.
DR   CDD; cd05016; SIS_PGI_2; 1.
DR   Gene3D; 1.10.1390.10; -; 1.
DR   HAMAP; MF_00473; G6P_isomerase; 1.
DR   InterPro; IPR001672; G6P_Isomerase.
DR   InterPro; IPR023096; G6P_Isomerase_C.
DR   InterPro; IPR018189; Phosphoglucose_isomerase_CS.
DR   InterPro; IPR046348; SIS_dom_sf.
DR   InterPro; IPR035476; SIS_PGI_1.
DR   InterPro; IPR035482; SIS_PGI_2.
DR   PANTHER; PTHR11469; PTHR11469; 1.
DR   Pfam; PF00342; PGI; 1.
DR   PRINTS; PR00662; G6PISOMERASE.
DR   SUPFAM; SSF53697; SSF53697; 1.
DR   PROSITE; PS00765; P_GLUCOSE_ISOMERASE_1; 1.
DR   PROSITE; PS00174; P_GLUCOSE_ISOMERASE_2; 1.
DR   PROSITE; PS51463; P_GLUCOSE_ISOMERASE_3; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Gluconeogenesis; Glycolysis; Isomerase.
FT   CHAIN           1..558
FT                   /note="Glucose-6-phosphate isomerase"
FT                   /id="PRO_0000180544"
FT   ACT_SITE        362
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        393
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        523
FT                   /evidence="ECO:0000250"
FT   VARIANT         12
FT                   /note="P -> A (in strain: various strains)"
FT   VARIANT         25
FT                   /note="A -> G (in strain: various strains)"
SQ   SEQUENCE   558 AA;  62349 MW;  D3E25A3F02327890 CRC64;
     MAGPLPPLNQ EPAFQKLQEY YDSKAKDLNI KDLFVKDSKR FSKYSLRLHT QNDGEILLDY
     SKNRINDEVW DLLLALAKVR RVNAARDAMF SGQHINITEN RAVLHTALRN RGTDPVLVDD
     KDVMPDVRAE LAHMKEFTNM VISGVWRGCT GKQITDVVNI GIGGSDLGPL MVTEALKPYG
     KGLHSHFVSN IDGTHLAEVL KKVNYETTLF IVASKTFTTQ ETITNATSAK TWLLEHSKEP
     ESVAKHFVAL STNKEKVTEF GIDSTNMFGF WDWVGGRYSL WSAIGLSICL SIGFENFEQL
     LDGAHFMDNH FKTTPFEKNA PVILALLGVW YSNFFKAETH ALLPYDQYLH RFAAYFQQGD
     MESNGKFVSK SGKPVKYSTG PIVWGEPGTN GQHAFYQLIH QGTRLIPCDF IAPAQTHNPI
     AGGKHHKILL SNFLAQTEAL MAGKTVDEAR TELSKAGLCG NELDNLLPHK VFVGNRPTNS
     IVVKKVSPFT LGALIALYEH KIFVQGIIWD INSFDQWGVE LGKQLAKAIE PELDHCNEVS
     THDSSTNGLI NFIKANWK