ID   G6PI_SPIOL              Reviewed;         566 AA.
AC   O82059;
DT   02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   21-JUN-2004, sequence version 2.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Glucose-6-phosphate isomerase, cytosolic;
DE            Short=GPI;
DE            EC=5.3.1.9;
DE   AltName: Full=Phosphoglucose isomerase;
DE            Short=PGI;
DE   AltName: Full=Phosphohexose isomerase;
DE            Short=PHI;
GN   Name=PGIC; Synonyms=GPIS;
OS   Spinacia oleracea (Spinach).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX   NCBI_TaxID=3562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9651529; DOI=10.1016/s0378-1119(98)00229-7;
RA   Nowitzki U., Flechner A., Kellermann J., Hasegawa M., Schnarrenberger C.,
RA   Martin W.;
RT   "Eubacterial origin of nuclear genes for chloroplast and cytosolic glucose-
RT   6-phosphate isomerase from spinach: sampling eubacterial gene diversity in
RT   eukaryotic chromosomes through symbiosis.";
RL   Gene 214:205-213(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate;
CC         Xref=Rhea:RHEA:11816, ChEBI:CHEBI:57634, ChEBI:CHEBI:58225;
CC         EC=5.3.1.9;
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 2/4.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GPI family. {ECO:0000305}.
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DR   EMBL; AJ000266; CAA03983.1; -; mRNA.
DR   PIR; T09154; T09154.
DR   AlphaFoldDB; O82059; -.
DR   SMR; O82059; -.
DR   OrthoDB; 446616at2759; -.
DR   SABIO-RK; O82059; -.
DR   UniPathway; UPA00109; UER00181.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR   GO; GO:0004347; F:glucose-6-phosphate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd05015; SIS_PGI_1; 1.
DR   CDD; cd05016; SIS_PGI_2; 1.
DR   Gene3D; 1.10.1390.10; -; 1.
DR   HAMAP; MF_00473; G6P_isomerase; 1.
DR   InterPro; IPR001672; G6P_Isomerase.
DR   InterPro; IPR023096; G6P_Isomerase_C.
DR   InterPro; IPR018189; Phosphoglucose_isomerase_CS.
DR   InterPro; IPR046348; SIS_dom_sf.
DR   InterPro; IPR035476; SIS_PGI_1.
DR   InterPro; IPR035482; SIS_PGI_2.
DR   PANTHER; PTHR11469; PTHR11469; 1.
DR   Pfam; PF00342; PGI; 1.
DR   PRINTS; PR00662; G6PISOMERASE.
DR   SUPFAM; SSF53697; SSF53697; 1.
DR   PROSITE; PS00765; P_GLUCOSE_ISOMERASE_1; 1.
DR   PROSITE; PS00174; P_GLUCOSE_ISOMERASE_2; 1.
DR   PROSITE; PS51463; P_GLUCOSE_ISOMERASE_3; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Gluconeogenesis; Glycolysis; Isomerase.
FT   CHAIN           1..566
FT                   /note="Glucose-6-phosphate isomerase, cytosolic"
FT                   /id="PRO_0000180568"
FT   ACT_SITE        360
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        391
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        516
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   566 AA;  62130 MW;  6079EE5D3A53460A CRC64;
     MAPSTLICDT DSWQNLKTHV AEIKKTHLRD LMSDADRCKS MMVEFDGLLL DYSRQNATHD
     TMSKLFQLAE ASHLKDKINQ MFNGEHINST ENRSVLHVAL RASRDAVING DGKNVVPDVW
     QVLDKIRDFS EKIRSGSWVG VTGKPLTNVV AVGIGGSFLG PLFVHTALQT ESEAAECAKG
     RQLRFLANVD PIDVAKNISG LNPETTLVVV VSKTFTTAET MLNARTLREW ISSALGPAAV
     AKHMVAVSTN LTLVEKFGID PKNAFAFWDW VGGRYSVCSA VGVLPLSLQY GFPIVEKFLK
     GASSIDQHFH SAPLEKNLPV LLGLLSLWNV SFLGHPARAI LPYCQALEKF APHIQQVSME
     SNGKGVSIDG VVLPFEAGEI DFGEPGTNGQ HSFYQLIHQG RVIPCDFIGI AKSQQPVYLK
     GEVVSNHDEL MSNFFAQPDA LAYGKTQEEL QKENISPHLV PHKTFTGNRP SLSLLLPSLT
     AYNVGQLLAI YEHRVAVEGF VWGINSFDQW GVELGKSLAN QVRKQLHASR TNGEAVKGFN
     FSTTTVMAKY LQETSDVPAE LPTKLP