ID   ALG5_DICDI              Reviewed;         327 AA.
AC   Q54J42; Q6TMJ2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Dolichyl-phosphate beta-glucosyltransferase;
DE            Short=DolP-glucosyltransferase;
DE            EC=2.4.1.117;
DE   AltName: Full=Asparagine-linked glycosylation protein 5 homolog;
GN   Name=alg5; Synonyms=dgtA; ORFNames=DDB_G0288321;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 39-327, AND INDUCTION.
RX   PubMed=15075287; DOI=10.1128/ec.3.2.564-566.2004;
RA   Escalante R., Iranfar N., Sastre L., Loomis W.F.;
RT   "Identification of genes dependent on the MADS box transcription factor
RT   SrfA in Dictyostelium discoideum development.";
RL   Eukaryot. Cell 3:564-566(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a dolichyl phosphate + UDP-alpha-D-glucose = a dolichyl beta-
CC         D-glucosyl phosphate + UDP; Xref=Rhea:RHEA:15401, Rhea:RHEA-
CC         COMP:9517, Rhea:RHEA-COMP:9528, ChEBI:CHEBI:57525, ChEBI:CHEBI:57683,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.117;
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type II membrane protein {ECO:0000250}.
CC   -!- INDUCTION: Regulated by srfA. {ECO:0000269|PubMed:15075287}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR   EMBL; AAFI02000111; EAL63257.1; -; Genomic_DNA.
DR   EMBL; AY392442; AAQ98885.1; -; Genomic_DNA.
DR   RefSeq; XP_636759.1; XM_631667.1.
DR   AlphaFoldDB; Q54J42; -.
DR   SMR; Q54J42; -.
DR   STRING; 44689.DDB0191396; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   PaxDb; Q54J42; -.
DR   EnsemblProtists; EAL63257; EAL63257; DDB_G0288321.
DR   GeneID; 8626562; -.
DR   KEGG; ddi:DDB_G0288321; -.
DR   dictyBase; DDB_G0288321; dgtA.
DR   eggNOG; KOG2977; Eukaryota.
DR   HOGENOM; CLU_033536_9_1_1; -.
DR   InParanoid; Q54J42; -.
DR   OMA; IVPYMHA; -.
DR   PhylomeDB; Q54J42; -.
DR   Reactome; R-DDI-480985; Synthesis of dolichyl-phosphate-glucose.
DR   UniPathway; UPA00378; -.
DR   PRO; PR:Q54J42; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004581; F:dolichyl-phosphate beta-glucosyltransferase activity; ISS:dictyBase.
DR   GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central.
DR   CDD; cd04188; DPG_synthase; 1.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR035518; DPG_synthase.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Glycosyltransferase; Membrane;
KW   Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..327
FT                   /note="Dolichyl-phosphate beta-glucosyltransferase"
FT                   /id="PRO_0000327383"
FT   TOPO_DOM        1..15
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        16..36
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        37..327
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        289
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   327 AA;  37636 MW;  3C071909E78A8527 CRC64;
     MIDLFINIAS FTIYGIPVIP LFIIVFVILS YYLLLLHDES PLWLEKENVY IDVKEGEQHQ
     FPSLVENKNP IDNIYLSVIV PAYNEQIRLP SMLDDAIKFL NEKSKKDLKF SYEIIIIDDG
     SKDSTAKLVT SYIEKQPSSN IRLLKLKQNR GKGGAVKRGI LCSRGKYCLM VDADGATEFK
     DFNRVEDIMH KIEKNDLGIV CGSRSHLVDS DLVAKRSFLR NILMYGFHIF VQTLCVKGIK
     DTQCGFKLFT RETARRIFPT LHIERWAFDV EILYLAQKLN IPIAEVAVNW TEIDGSKLDP
     FSSSIQMAKD IVRIRFRYLL GIWKIKS