G6PI_THET8
ID G6PI_THET8 Reviewed; 415 AA.
AC Q5SLL6;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Glucose-6-phosphate isomerase {ECO:0000255|HAMAP-Rule:MF_00473};
DE Short=GPI {ECO:0000255|HAMAP-Rule:MF_00473};
DE EC=5.3.1.9 {ECO:0000255|HAMAP-Rule:MF_00473};
DE AltName: Full=Phosphoglucose isomerase {ECO:0000255|HAMAP-Rule:MF_00473};
DE Short=PGI {ECO:0000255|HAMAP-Rule:MF_00473};
DE AltName: Full=Phosphohexose isomerase {ECO:0000255|HAMAP-Rule:MF_00473};
DE Short=PHI {ECO:0000255|HAMAP-Rule:MF_00473};
GN Name=pgi {ECO:0000255|HAMAP-Rule:MF_00473}; OrderedLocusNames=TTHA0277;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
RG RIKEN structural genomics initiative (RSGI);
RT "Crystal structure of hypothetical protein TT0277 from Thermus thermophilus
RT HB8.";
RL Submitted (JUN-2006) to the PDB data bank.
CC -!- FUNCTION: Catalyzes the reversible isomerization of glucose-6-phosphate
CC to fructose-6-phosphate. {ECO:0000255|HAMAP-Rule:MF_00473}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate;
CC Xref=Rhea:RHEA:11816, ChEBI:CHEBI:57634, ChEBI:CHEBI:58225;
CC EC=5.3.1.9; Evidence={ECO:0000255|HAMAP-Rule:MF_00473};
CC -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC {ECO:0000255|HAMAP-Rule:MF_00473}.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 2/4.
CC {ECO:0000255|HAMAP-Rule:MF_00473}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00473}.
CC -!- SIMILARITY: Belongs to the GPI family. {ECO:0000255|HAMAP-
CC Rule:MF_00473}.
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DR EMBL; AP008226; BAD70100.1; -; Genomic_DNA.
DR RefSeq; WP_011227825.1; NC_006461.1.
DR RefSeq; YP_143543.1; NC_006461.1.
DR PDB; 1ZZG; X-ray; 1.95 A; A/B=1-415.
DR PDBsum; 1ZZG; -.
DR AlphaFoldDB; Q5SLL6; -.
DR SMR; Q5SLL6; -.
DR STRING; 300852.55771659; -.
DR EnsemblBacteria; BAD70100; BAD70100; BAD70100.
DR GeneID; 3168044; -.
DR KEGG; ttj:TTHA0277; -.
DR PATRIC; fig|300852.9.peg.277; -.
DR eggNOG; COG0166; Bacteria.
DR HOGENOM; CLU_037303_1_0_0; -.
DR OMA; IGVWYIN; -.
DR PhylomeDB; Q5SLL6; -.
DR UniPathway; UPA00109; UER00181.
DR UniPathway; UPA00138; -.
DR EvolutionaryTrace; Q5SLL6; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR GO; GO:0004347; F:glucose-6-phosphate isomerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniRule.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniRule.
DR CDD; cd05015; SIS_PGI_1; 1.
DR CDD; cd05016; SIS_PGI_2; 1.
DR HAMAP; MF_00473; G6P_isomerase; 1.
DR InterPro; IPR001672; G6P_Isomerase.
DR InterPro; IPR018189; Phosphoglucose_isomerase_CS.
DR InterPro; IPR046348; SIS_dom_sf.
DR InterPro; IPR035476; SIS_PGI_1.
DR InterPro; IPR035482; SIS_PGI_2.
DR PANTHER; PTHR11469; PTHR11469; 1.
DR Pfam; PF00342; PGI; 1.
DR PRINTS; PR00662; G6PISOMERASE.
DR SUPFAM; SSF53697; SSF53697; 1.
DR PROSITE; PS00174; P_GLUCOSE_ISOMERASE_2; 1.
DR PROSITE; PS51463; P_GLUCOSE_ISOMERASE_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Gluconeogenesis; Glycolysis; Isomerase;
KW Reference proteome.
FT CHAIN 1..415
FT /note="Glucose-6-phosphate isomerase"
FT /id="PRO_0000180759"
FT ACT_SITE 267
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00473"
FT ACT_SITE 293
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00473"
FT ACT_SITE 406
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00473"
FT STRAND 3..5
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 12..18
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 20..32
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 34..36
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 42..44
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 52..61
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 67..72
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 75..77
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 79..88
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 94..98
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 103..112
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 115..117
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 118..127
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 130..147
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 148..154
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 155..159
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 161..164
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 165..173
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 176..179
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 186..188
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 193..201
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 206..221
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 224..226
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 228..238
FT /evidence="ECO:0007829|PDB:1ZZG"
FT TURN 239..241
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 242..249
FT /evidence="ECO:0007829|PDB:1ZZG"
FT TURN 253..256
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 257..269
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 270..272
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 282..287
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 290..292
FT /evidence="ECO:0007829|PDB:1ZZG"
FT TURN 293..295
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 296..301
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 306..314
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 329..331
FT /evidence="ECO:0007829|PDB:1ZZG"
FT TURN 332..336
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 339..356
FT /evidence="ECO:0007829|PDB:1ZZG"
FT STRAND 360..370
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 371..391
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 400..402
FT /evidence="ECO:0007829|PDB:1ZZG"
FT HELIX 403..414
FT /evidence="ECO:0007829|PDB:1ZZG"
SQ SEQUENCE 415 AA; 46081 MW; A8C23A5C2E3EFC56 CRC64;
MLRLDTRFLP GFPEALSRHG PLLEEARRRL LAKRGEPGSM LGWMDLPEDT ETLREVRRYR
EANPWVEDFV LIGIGGSALG PKALEAAFNE SGVRFHYLDH VEPEPILRLL RTLDPRKTLV
NAVSKSGSTA ETLAGLAVFL KWLKAHLGED WRRHLVVTTD PKEGPLRAFA EREGLKAFAI
PKEVGGRFSA LSPVGLLPLA FAGADLDALL MGARKANETA LAPLEESLPL KTALLLHLHR
HLPVHVFMVY SERLSHLPSW FVQLHDESLG KVDRQGQRVG TTAVPALGPK DQHAQVQLFR
EGPLDKLLAL VIPEAPLEDV EIPEVEGLEA ASYLFGKTLF QLLKAEAEAT YEALAEAGQR
VYALFLPEVS PYAVGWLMQH LMWQTAFLGE LWEVNAFDQP GVELGKVLTR KRLAG
