ID   3NO22_NAJAT             Reviewed;          86 AA.
AC   Q9YGI4; P82012; Q9YGI7; Q9YGJ3;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Probable weak neurotoxin NNAM2;
DE   AltName: Full=TA-N9;
DE   Flags: Precursor;
OS   Naja atra (Chinese cobra).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8656;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=9838137; DOI=10.1016/s0167-4781(98)00218-8;
RA   Qian Y.-C., Fan C.-Y., Gong Y., Yang S.-L.;
RT   "cDNA sequence analysis and expression of four long neurotoxin homologues
RT   from Naja naja atra.";
RL   Biochim. Biophys. Acta 1443:233-238(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Chang L.-S.;
RT   "A novel neurotoxin from Naja naja atra.";
RL   Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PROTEIN SEQUENCE OF 22-86, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RA   Chang L.-S.;
RT   "A novel neurotoxin from Naja naja atra (Taiwan cobra) venom.";
RL   Submitted (AUG-1999) to UniProtKB.
CC   -!- FUNCTION: Binds with low affinity to muscular (alpha-1-beta-1-delta-
CC       epsilon/CHRNA1-CHRNB1-CHRND-CHRNE) and very low affinity to neuronal
CC       (alpha-7/CHRNA7) nicotinic acetylcholine receptor (nAChR).
CC       {ECO:0000250|UniProtKB:O42255}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.3}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Ancestral
CC       subfamily. Orphan group II sub-subfamily. {ECO:0000305}.
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DR   EMBL; AJ001185; CAA04578.1; -; mRNA.
DR   EMBL; AJ131926; CAA10530.1; -; mRNA.
DR   AlphaFoldDB; Q9YGI4; -.
DR   SMR; Q9YGI4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|Ref.3"
FT   CHAIN           22..86
FT                   /note="Probable weak neurotoxin NNAM2"
FT                   /evidence="ECO:0000269|Ref.3"
FT                   /id="PRO_0000035469"
FT   DISULFID        24..45
FT                   /evidence="ECO:0000250|UniProtKB:Q8AY51"
FT   DISULFID        27..32
FT                   /evidence="ECO:0000250|UniProtKB:Q8AY51"
FT   DISULFID        38..63
FT                   /evidence="ECO:0000250|UniProtKB:Q8AY51"
FT   DISULFID        67..78
FT                   /evidence="ECO:0000250|UniProtKB:Q8AY51"
FT   DISULFID        79..84
FT                   /evidence="ECO:0000250|UniProtKB:Q8AY51"
FT   CONFLICT        7
FT                   /note="T -> S (in Ref. 1; CAA04578)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   86 AA;  9899 MW;  0EB7CB3EB6C407F2 CRC64;
     MKTLLLTLVV VTIVCLDLGY TLTCLNCPEM FCGKFQICRN GEKICFKKLH QRRPFSLRYI
     RGCAATCPET KPRDMVECCS TDRCNR