ID   G7_VACCW                Reviewed;         371 AA.
AC   P68716; Q80HW8;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   29-SEP-2021, entry version 42.
DE   RecName: Full=Assembly protein G7;
GN   OrderedLocusNames=VACWR085; ORFNames=G7L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2024483; DOI=10.1016/0042-6822(91)90585-y;
RA   Meis R.J., Condit R.C.;
RT   "Genetic and molecular biological characterization of a vaccinia virus gene
RT   which renders the virus dependent on isatin-beta-thiosemicarbazone (IBT).";
RL   Virology 182:442-454(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   IDENTIFICATION IN COMPLEX WITH A30 AND F10, AND SUBCELLULAR LOCATION.
RX   PubMed=14671108; DOI=10.1128/jvi.78.1.266-274.2004;
RA   Szajner P., Weisberg A.S., Moss B.;
RT   "Physical and functional interactions between vaccinia virus F10 protein
RT   kinase and virion assembly proteins A30 and G7.";
RL   J. Virol. 78:266-274(2004).
RN   [4]
RP   IDENTIFICATION IN COMPLEX WITH A30; F10; A15; D2; D3 AND J1, FUNCTION, AND
RP   INDUCTION.
RX   PubMed=15567438; DOI=10.1016/j.virol.2004.10.008;
RA   Szajner P., Jaffe H., Weisberg A.S., Moss B.;
RT   "A complex of seven vaccinia virus proteins conserved in all
RT   chordopoxviruses is required for the association of membranes and viroplasm
RT   to form immature virions.";
RL   Virology 330:447-459(2004).
RN   [5]
RP   PHOSPHORYLATION, AND PROTEOLYTIC CLEAVAGE.
RX   PubMed=15890954; DOI=10.1128/jvi.79.11.7146-7161.2005;
RA   Mercer J., Traktman P.;
RT   "Genetic and cell biological characterization of the vaccinia virus A30 and
RT   G7 phosphoproteins.";
RL   J. Virol. 79:7146-7161(2005).
CC   -!- FUNCTION: Late protein which is a part of a large complex required for
CC       early virion morphogenesis. This complex participates in the formation
CC       of virosomes and the incorporation of virosomal contents into nascent
CC       immature virions. {ECO:0000269|PubMed:15567438}.
CC   -!- SUBUNIT: Part of a complex composed of A30, G7, F10 kinase, A15, D2,
CC       D3, and J1. {ECO:0000269|PubMed:14671108, ECO:0000269|PubMed:15567438}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}. Virion
CC       {ECO:0000250}. Note=Localizes in cytoplasmic virus factories and
CC       present in the virion core. {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:15567438}.
CC   -!- PTM: Phosphorylated on serines by F10 kinase, phosphorylation state is
CC       regulated by H1 phosphatase. {ECO:0000305|PubMed:15890954}.
CC   -!- PTM: Undergoes proteolytic processing during morphogenesis, probably
CC       required for the transformation of immature virions (IV) into mature
CC       virions (MV). {ECO:0000305|PubMed:15890954}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae G7 family. {ECO:0000305}.
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DR   EMBL; J03399; AAB59818.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89364.1; -; Genomic_DNA.
DR   RefSeq; YP_232967.1; NC_006998.1.
DR   DNASU; 3707541; -.
DR   GeneID; 3707541; -.
DR   KEGG; vg:3707541; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008787; Poxvirus_G7.
DR   Pfam; PF05503; Pox_G7; 1.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Phosphoprotein; Reference proteome; Virion.
FT   CHAIN           1..371
FT                   /note="Assembly protein G7"
FT                   /id="PRO_0000099538"
FT   SITE            187..188
FT                   /note="Cleavage; by I7 protease"
FT                   /evidence="ECO:0000305"
FT   SITE            238..239
FT                   /note="Cleavage; by I7 protease"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   371 AA;  41951 MW;  CEEF21179B65947E CRC64;
     MAAEQRRSTI FDIVSKCIVQ SVLRDISINS EYIESKAKQL CYCPASKKES VINGIYNCCE
     SNIEIMDKEQ LLKILDNLRC HSAHVCNATD FWRLYNSLKR FTHTTAFFNT CKPTILATLN
     TLITLILSNK LLYAAEMVEY LENQLDSSNK SMSQELAELL EMKYALINLV QYRILPMIIG
     EPIIVAGFSG KEPISDYSAE VERLMELPVK TDIVNTTYDF LARKGIDTSN NIAEYIAGLK
     IEEIEKVEKY LPEVISTIAN SNIIKNKKSI FPANINDKQI MECSRMLDTS EKYSKGYKTD
     GAVTSPLTGN NTITTFIPIS ASDMQKFTIL EYLYIMRVMA NNVKKKNEGK NNGGVVMHIN
     SPFKVINLPK C