G9_VACCW
ID G9_VACCW Reviewed; 340 AA.
AC P07611; Q76ZT8; Q85328;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Myristoylated protein G9;
DE AltName: Full=Protein F1;
GN OrderedLocusNames=VACWR087; ORFNames=G9R;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-340.
RX PubMed=2987815; DOI=10.1093/nar/13.3.985;
RA Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E.;
RT "Nucleotide sequence of a cluster of early and late genes in a conserved
RT segment of the vaccinia virus genome.";
RL Nucleic Acids Res. 13:985-998(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
RX PubMed=2024483; DOI=10.1016/0042-6822(91)90585-y;
RA Meis R.J., Condit R.C.;
RT "Genetic and molecular biological characterization of a vaccinia virus gene
RT which renders the virus dependent on isatin-beta-thiosemicarbazone (IBT).";
RL Virology 182:442-454(1991).
RN [4]
RP MYRISTOYLATION AT GLY-2, AND MUTAGENESIS OF GLY-2.
RX PubMed=9188589; DOI=10.1128/jvi.71.7.5218-5226.1997;
RA Martin K.H., Grosenbach D.W., Franke C.A., Hruby D.E.;
RT "Identification and analysis of three myristylated vaccinia virus late
RT proteins.";
RL J. Virol. 71:5218-5226(1997).
RN [5]
RP IDENTIFICATION IN A COMPLEX WITH A16; A21; A28; G3; H2; J5 AND L5.
RX PubMed=16339313; DOI=10.1073/pnas.0509239102;
RA Senkevich T.G., Ojeda S., Townsley A., Nelson G.E., Moss B.;
RT "Poxvirus multiprotein entry-fusion complex.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:18572-18577(2005).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16973586; DOI=10.1128/jvi.00987-06;
RA Ojeda S., Domi A., Moss B.;
RT "Vaccinia virus G9 protein is an essential component of the poxvirus entry-
RT fusion complex.";
RL J. Virol. 80:9822-9830(2006).
RN [7]
RP INTERACTION WITH A16.
RX PubMed=18353946; DOI=10.1128/jvi.00162-08;
RA Wagenaar T.R., Ojeda S., Moss B.;
RT "Vaccinia virus A56/K2 fusion regulatory protein interacts with the A16 and
RT G9 subunits of the entry fusion complex.";
RL J. Virol. 82:5153-5160(2008).
CC -!- FUNCTION: Envelope protein part of the entry-fusion complex responsible
CC for the virus membrane fusion with host cell membrane during virus
CC entry. Also plays a role in cell-cell fusion (syncytium formation)
CC (Probable). {ECO:0000305|PubMed:16973586}.
CC -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at least
CC composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation
CC of the viral membrane is necessary for the assembly of the complex (By
CC similarity). Interacts with A16; this heterodimer further interact with
CC A56/K2 fusion regulatory heterodimer. {ECO:0000250,
CC ECO:0000269|PubMed:16339313, ECO:0000269|PubMed:18353946}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC II membrane protein {ECO:0000305}. Note=Component of the mature virion
CC (MV) membrane. The mature virion is located in the cytoplasm of
CC infected cells and is probably released by cell lysis.
CC {ECO:0000269|PubMed:16973586}.
CC -!- SIMILARITY: Belongs to the poxviridae A16/G9/J5 family. {ECO:0000305}.
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DR EMBL; AY243312; AAO89366.1; -; Genomic_DNA.
DR EMBL; X01978; CAA26009.1; -; Genomic_DNA.
DR EMBL; J03399; AAB59820.1; -; Genomic_DNA.
DR PIR; A23092; QQVZF1.
DR RefSeq; YP_232969.1; NC_006998.1.
DR IntAct; P07611; 3.
DR MINT; P07611; -.
DR TCDB; 1.G.11.1.1; the poxvirus cell entry protein complex (pep-c) family.
DR iPTMnet; P07611; -.
DR DNASU; 3707543; -.
DR GeneID; 3707543; -.
DR KEGG; vg:3707543; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR InterPro; IPR004251; Pox_virus_G9/A16.
DR Pfam; PF03003; Pox_G9-A16; 1.
PE 1: Evidence at protein level;
KW Fusion of virus membrane with host cell membrane;
KW Fusion of virus membrane with host membrane; Lipoprotein; Membrane;
KW Myristate; Reference proteome; Signal-anchor; Transmembrane;
KW Transmembrane helix; Viral envelope protein;
KW Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT INIT_MET 1
FT /note="Removed; by host"
FT CHAIN 2..340
FT /note="Myristoylated protein G9"
FT /id="PRO_0000099543"
FT TOPO_DOM 2..319
FT /note="Virion surface"
FT TRANSMEM 320..340
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 2
FT /note="N-myristoyl glycine; by host"
FT /evidence="ECO:0000269|PubMed:9188589"
FT MUTAGEN 2
FT /note="G->A: Complete loss of myristoylation."
FT /evidence="ECO:0000269|PubMed:9188589"
FT CONFLICT 140
FT /note="F -> S (in Ref. 2; CAA26009)"
FT /evidence="ECO:0000305"
FT CONFLICT 320
FT /note="L -> S (in Ref. 2; CAA26009)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 340 AA; 38786 MW; 025399172AB9ABC4 CRC64;
MGGGVSVELP KRDPPPGVPT DEMLLNVDKM HDVIAPAKLL EYVHIGPLAK DKEDKVKKRY
PEFRLVNTGP GGLSALLRQS YNGTAPNCCR TFNRTHYWKK DGKISDKYEE GAVLESCWPD
VHDTGKCDVD LFDWCQGDTF DRNICHQWIG SAFNRSNRTV EGQQSLINLY NKMQTLCSKD
ASVPICESFL HHLRAHNTED SKEMIDYILR QQSADFKQKY MRCSYPTRDK LEESLKYAEP
RECWDPECSN ANVNFLLTRN YNNLGLCNIV RCNTSVNNLQ MDKTSSLRLS CGLSNSDRFS
TVPVNRAKVV QHNIKHSFDL KLHLISLLSL LVIWILIVAI
