ID   G9_VAR67                Reviewed;         340 AA.
AC   P0DOS7; P32998; Q76Q19;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Myristoylated protein G9;
GN   ORFNames=G9R, H9R, I10R;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8383392; DOI=10.1016/0168-1702(93)90110-9;
RA   Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S.,
RA   Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V.,
RA   Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G.,
RA   Sandakhchiev L.S.;
RT   "Nucleotide sequence analysis of variola virus HindIII M, L, I genome
RT   fragments.";
RL   Virus Res. 27:25-35(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Envelope protein part of the entry-fusion complex responsible
CC       for the virus membrane fusion with host cell membrane during virus
CC       entry. Also plays a role in cell-cell fusion (syncytium formation) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at least
CC       composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation
CC       of the viral membrane is necessary for the assembly of the complex.
CC       Interacts with A16; this heterodimer further interact with A56/K2
CC       fusion regulatory heterodimer (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Single-pass type
CC       II membrane protein {ECO:0000305}. Note=Component of the mature virion
CC       (MV) membrane. The mature virion is located in the cytoplasm of
CC       infected cells and is probably released by cell lysis (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae A16/G9/J5 family. {ECO:0000305}.
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DR   EMBL; X67119; CAA47571.1; -; Genomic_DNA.
DR   EMBL; S55844; AAB24668.1; -; Genomic_DNA.
DR   EMBL; X69198; CAA49013.1; -; Genomic_DNA.
DR   PIR; S33086; S33086.
DR   RefSeq; NP_042116.1; NC_001611.1.
DR   GeneID; 1486438; -.
DR   KEGG; vg:1486438; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR004251; Pox_virus_G9/A16.
DR   Pfam; PF03003; Pox_G9-A16; 1.
PE   3: Inferred from homology;
KW   Fusion of virus membrane with host cell membrane;
KW   Fusion of virus membrane with host membrane; Lipoprotein; Membrane;
KW   Myristate; Reference proteome; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Viral envelope protein;
KW   Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT   INIT_MET        1
FT                   /note="Removed; by host"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..340
FT                   /note="Myristoylated protein G9"
FT                   /id="PRO_0000099544"
FT   TOPO_DOM        2..319
FT                   /note="Virion surface"
FT   TRANSMEM        320..340
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           2
FT                   /note="N-myristoyl glycine; by host"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   340 AA;  38766 MW;  1217D665801A2343 CRC64;
     MGGGVSVELP KRDPPPGVPT DEMLLNVDKM HDVIAPAKLL EYVHIGPLAK DKEDKVKKRY
     PEFRLVNTGP GGLSALLRQS YNGTAPNCCH TFNRTHYWKK DGKISDKYEE GAVLESCWPD
     VHDTGKCDVN LFDWCQGDTF DRNICHQWIG SAFNRSDRTV EGQQSLINLY NKMQTLCSKD
     ASVPICESFL HHLRAHNTED SKEMIDYILR QQSANFKQKY MRCSYPTRDK LEESLKYAEP
     RECWDPECSN ANVNFLLTRN YNNLGLCNIV RCNTSVNNLQ MDKTSSLRLS CGLSNSDRFS
     TVPVNRAKVV QHNIKHSFDL KLHLISLLSL LVIWILIVAI