GA2PE_DROME
ID GA2PE_DROME Reviewed; 977 AA.
AC Q9W3Y4; Q95TD5;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=GAS2-like protein pickled eggs {ECO:0000305};
GN Name=pigs {ECO:0000312|FlyBase:FBgn0029881};
GN ORFNames=CG3973 {ECO:0000312|FlyBase:FBgn0029881};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAO39480.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAO39480.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAO39480.1};
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000312|EMBL:AAL13365.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL13365.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=20150280; DOI=10.1242/dev.043224;
RA Pines M.K., Housden B.E., Bernard F., Bray S.J., Roeper K.;
RT "The cytolinker Pigs is a direct target and a negative regulator of Notch
RT signalling.";
RL Development 137:913-922(2010).
CC -!- FUNCTION: Essential for development and viability. Required for ovary
CC development and oogenesis, and is essential for the development of the
CC indirect flight muscles. May act as a negative regulator of the Notch
CC signaling pathway in certain tissues, such as the muscle precursors and
CC ovaries. May function as a linker protein between the actin and
CC microtubule cytoskeletons. {ECO:0000269|PubMed:20150280}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:20150280}. Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:20150280}. Note=In the cytoskeleton associates with
CC both actin and the microtubules. {ECO:0000269|PubMed:20150280}.
CC -!- TISSUE SPECIFICITY: Expressed in the ovary and the ring canals of the
CC germline cells. In larvae, expressed in the notal region of the wing
CC disk. {ECO:0000269|PubMed:20150280}.
CC -!- MISCELLANEOUS: Named 'Picked eggs' based upon the mutant ovary
CC phenotype. {ECO:0000269|PubMed:20150280}.
CC -!- SIMILARITY: Belongs to the GAS2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL13365.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014298; AAF46177.2; -; Genomic_DNA.
DR EMBL; AE014298; AFH07264.1; -; Genomic_DNA.
DR EMBL; AE014298; AHN59420.1; -; Genomic_DNA.
DR EMBL; AY059459; AAL13365.1; ALT_INIT; mRNA.
DR EMBL; BT003477; AAO39480.1; -; mRNA.
DR RefSeq; NP_001245550.1; NM_001258621.2.
DR RefSeq; NP_001284949.1; NM_001298020.1.
DR RefSeq; NP_572331.2; NM_132103.3.
DR AlphaFoldDB; Q9W3Y4; -.
DR SMR; Q9W3Y4; -.
DR IntAct; Q9W3Y4; 5.
DR STRING; 7227.FBpp0070909; -.
DR PaxDb; Q9W3Y4; -.
DR PRIDE; Q9W3Y4; -.
DR EnsemblMetazoa; FBtr0070948; FBpp0070909; FBgn0029881.
DR EnsemblMetazoa; FBtr0308653; FBpp0300876; FBgn0029881.
DR EnsemblMetazoa; FBtr0343375; FBpp0310031; FBgn0029881.
DR GeneID; 31596; -.
DR KEGG; dme:Dmel_CG3973; -.
DR UCSC; CG3973-RA; d. melanogaster.
DR CTD; 94005; -.
DR FlyBase; FBgn0029881; pigs.
DR VEuPathDB; VectorBase:FBgn0029881; -.
DR eggNOG; KOG0516; Eukaryota.
DR GeneTree; ENSGT00940000154849; -.
DR HOGENOM; CLU_013204_0_0_1; -.
DR InParanoid; Q9W3Y4; -.
DR OMA; MVRDVTN; -.
DR OrthoDB; 1343422at2759; -.
DR PhylomeDB; Q9W3Y4; -.
DR SignaLink; Q9W3Y4; -.
DR BioGRID-ORCS; 31596; 0 hits in 3 CRISPR screens.
DR ChiTaRS; pigs; fly.
DR GenomeRNAi; 31596; -.
DR PRO; PR:Q9W3Y4; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0029881; Expressed in brain and 24 other tissues.
DR GO; GO:0005884; C:actin filament; IBA:GO_Central.
DR GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0008093; F:cytoskeletal anchor activity; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0051764; P:actin crosslink formation; IBA:GO_Central.
DR GO; GO:0001578; P:microtubule bundle formation; IBA:GO_Central.
DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IMP:FlyBase.
DR GO; GO:0030707; P:ovarian follicle cell development; IMP:FlyBase.
DR GO; GO:0030713; P:ovarian follicle cell stalk formation; IMP:FlyBase.
DR GO; GO:1904825; P:protein localization to microtubule plus-end; IBA:GO_Central.
DR GO; GO:0031110; P:regulation of microtubule polymerization or depolymerization; IBA:GO_Central.
DR CDD; cd00014; CH; 1.
DR Gene3D; 1.10.418.10; -; 1.
DR Gene3D; 3.30.920.20; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR003108; GAR_dom.
DR InterPro; IPR036534; GAR_dom_sf.
DR Pfam; PF00307; CH; 1.
DR Pfam; PF02187; GAS2; 1.
DR SMART; SM00033; CH; 1.
DR SMART; SM00243; GAS2; 1.
DR SUPFAM; SSF143575; SSF143575; 1.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS50021; CH; 1.
DR PROSITE; PS51460; GAR; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Cytoskeleton; Reference proteome.
FT CHAIN 1..977
FT /note="GAS2-like protein pickled eggs"
FT /evidence="ECO:0000305"
FT /id="PRO_0000436918"
FT DOMAIN 20..159
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT DOMAIN 252..324
FT /note="GAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00792"
FT REGION 218..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 397..543
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 557..624
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 666..685
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 693..803
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 910..977
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..235
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 399..462
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 477..491
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 557..593
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 606..620
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 666..681
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 693..713
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 726..768
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 782..803
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 910..946
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 873
FT /note="T -> I (in Ref. 4; AAL13365)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 977 AA; 104466 MW; A0EDBEB4EDE851DA CRC64;
MAMLEARPYR PFKSSEEYLE AMREDLAEWL STLYPELSIN ADNFMDRLDT GVALCKHANY
VRQAAVDYLA RRQARNKSMT RSMTSGLAGP ILAMGNVHYL PAAKSGTFFA RDNVSNFITW
CRKSLKIIEC LLFETDDLIM RKNEKHVILC LLEVARRGAK FGMLAPMLVQ MERQIDREIA
ADIKANGAGC SENGTQTDAL ETGNSSAATM TTITTTTVET DLYDDSDDSE TEDDGDQNPV
LMYGPQPQII TNDLKSLDEM VRDLVEKCTC PSQFPMVRVS EGKYRIGDTK VLIFVRILRS
HVMVRVGGGW DTLSHYLDKH DPCRCRAQHR SSVAARLIPR QSPNHNPSNG IELHKAQVIF
ERSPPAARRV FNSPNCNGGP GTGSSCGTGV VGVAVPPTLQ NGHSLSPNSG KYRSRSPTPQ
RKFLNQQANG GGIASATGSS QTVTTDTSSG QLLGSPSLAR RSMSPSPRRL IDMRKKQSSL
DSYSSGPKSL PGYSCSMEEA NGGSGVGSAA GGVSSGSAGS GVAGEQGGAN KFENISDNGS
EISDEGYRSL GVIQSGAQKR ESLHSQASIE DAESNARLDQ TSSDSQISPS DEPAEKATTD
ILEEEDLNGQ DREEDQEDYS VCDGPQSLPA ILSGAHKLSD KFEQSGVFIT DDEITVDIAK
TEDQSVANTM GNPTPNLSKI PRSPLAQRRR RSIDNSTCGG AGGSLQDLSS RSGLPAPAFS
RKQPVYRSVR TRNSTGATTT PVAPPRSRQA TQLPMVRDVT NTWSGRTTGA PKRRPPCTAD
TFVAPTNGTG PAGSFERNGK GRSSQILYDS NGRRVRSGAP GCTSSLTTSP VKNHASSPLA
QQLLEAASSA KNDAQILEKM KSLLSRYAAG NQTKAGVGAT ATAANKINSN GKKTPVYEDF
TTAWVHSNGN LERSESCSPP AKARSKRSSA ASSCESNNSN AGAGSGAAAG SASVVSPRRE
RGMSKIPAPV RHHTELY
