GA3_GIBF5
ID GA3_GIBF5 Reviewed; 440 AA.
AC S0E2U7;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 1.
DT 03-AUG-2022, entry version 30.
DE RecName: Full=Cytochrome P450 monooygenase 1 {ECO:0000303|PubMed:9917370};
DE Short=P450-1 {ECO:0000303|PubMed:9917370};
DE EC=1.-.-.- {ECO:0000269|PubMed:11320210};
DE AltName: Full=Gibberellin cluster GA14 synthase {ECO:0000303|PubMed:11320210};
GN Name=P450-1 {ECO:0000303|PubMed:9917370}; ORFNames=FFUJ_14333;
OS Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae
OS and foot rot disease fungus) (Fusarium fujikuroi).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium fujikuroi species complex.
OX NCBI_TaxID=1279085;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND FUNCTION.
RC STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831;
RX PubMed=23825955; DOI=10.1371/journal.ppat.1003475;
RA Wiemann P., Sieber C.M.K., von Bargen K.W., Studt L., Niehaus E.-M.,
RA Espino J.J., Huss K., Michielse C.B., Albermann S., Wagner D.,
RA Bergner S.V., Connolly L.R., Fischer A., Reuter G., Kleigrewe K., Bald T.,
RA Wingfield B.D., Ophir R., Freeman S., Hippler M., Smith K.M., Brown D.W.,
RA Proctor R.H., Muensterkoetter M., Freitag M., Humpf H.-U., Gueldener U.,
RA Tudzynski B.;
RT "Deciphering the cryptic genome: genome-wide analyses of the rice pathogen
RT Fusarium fujikuroi reveal complex regulation of secondary metabolism and
RT novel metabolites.";
RL PLoS Pathog. 9:E1003475-E1003475(2013).
RN [2]
RP FUNCTION.
RX PubMed=9745028; DOI=10.1007/s002940050392;
RA Tudzynski B., Kawaide H., Kamiya Y.;
RT "Gibberellin biosynthesis in Gibberella fujikuroi: cloning and
RT characterization of the copalyl diphosphate synthase gene.";
RL Curr. Genet. 34:234-240(1998).
RN [3]
RP FUNCTION, INDUCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX PubMed=9917370; DOI=10.1006/fgbi.1998.1095;
RA Tudzynski B., Hoelter K.;
RT "Gibberellin biosynthetic pathway in Gibberella fujikuroi: evidence for a
RT gene cluster.";
RL Fungal Genet. Biol. 25:157-170(1998).
RN [4]
RP FUNCTION.
RX PubMed=10347043; DOI=10.1128/aem.65.6.2558-2564.1999;
RA Linnemannstoens P., Voss T., Hedden P., Gaskin P., Tudzynski B.;
RT "Deletions in the gibberellin biosynthesis gene cluster of Gibberella
RT fujikuroi by restriction enzyme-mediated integration and conventional
RT transformation-mediated mutagenesis.";
RL Appl. Environ. Microbiol. 65:2558-2564(1999).
RN [5]
RP FUNCTION.
RX PubMed=10803977; DOI=10.1271/bbb.64.660;
RA Toyomasu T., Kawaide H., Ishizaki A., Shinoda S., Otsuka M., Mitsuhashi W.,
RA Sassa T.;
RT "Cloning of a full-length cDNA encoding ent-kaurene synthase from
RT Gibberella fujikuroi: functional analysis of a bifunctional diterpene
RT cyclase.";
RL Biosci. Biotechnol. Biochem. 64:660-664(2000).
RN [6]
RP FUNCTION.
RX PubMed=11472927; DOI=10.1128/aem.67.8.3514-3522.2001;
RA Tudzynski B., Hedden P., Carrera E., Gaskin P.;
RT "The P450-4 gene of Gibberella fujikuroi encodes ent-kaurene oxidase in the
RT gibberellin biosynthesis pathway.";
RL Appl. Environ. Microbiol. 67:3514-3522(2001).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=11320210; DOI=10.1073/pnas.091096298;
RA Rojas M.C., Hedden P., Gaskin P., Tudzynski B.;
RT "The P450-1 gene of Gibberella fujikuroi encodes a multifunctional enzyme
RT in gibberellin biosynthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:5838-5843(2001).
RN [8]
RP FUNCTION.
RX PubMed=11943776; DOI=10.1074/jbc.m201651200;
RA Tudzynski B., Rojas M.C., Gaskin P., Hedden P.;
RT "The gibberellin 20-oxidase of Gibberella fujikuroi is a multifunctional
RT monooxygenase.";
RL J. Biol. Chem. 277:21246-21253(2002).
RN [9]
RP FUNCTION.
RX PubMed=12750377; DOI=10.1074/jbc.m301927200;
RA Tudzynski B., Mihlan M., Rojas M.C., Linnemannstons P., Gaskin P.,
RA Hedden P.;
RT "Characterization of the final two genes of the gibberellin biosynthesis
RT gene cluster of Gibberella fujikuroi: des and P450-3 encode GA4 desaturase
RT and the 13-hydroxylase, respectively.";
RL J. Biol. Chem. 278:28635-28643(2003).
RN [10]
RP FUNCTION.
RX PubMed=15925394; DOI=10.1016/j.phytochem.2005.04.012;
RA Malonek S., Boemke C., Bornberg-Bauer E., Rojas M.C., Hedden P.,
RA Hopkins P., Tudzynski B.;
RT "Distribution of gibberellin biosynthetic genes and gibberellin production
RT in the Gibberella fujikuroi species complex.";
RL Phytochemistry 66:1296-1311(2005).
CC -!- FUNCTION: GA14 synthase; part of the gene cluster that mediates the
CC biosynthesis of gibberellins (GAs), diterpenoids that may provide a
CC selective advantage during infection of the preferred host plant, rice
CC (PubMed:23825955, PubMed:9917370, PubMed:10347043, PubMed:12750377,
CC PubMed:15925394). Gibberellins (GAs) are diterpenoids and are
CC synthesized via the mevalonate pathway (PubMed:12750377). Biosynthesis
CC of the major metabolite GA3 (gibberellic acid) from geranylgeranyl
CC diphosphate (GGPP) requires 13 steps (PubMed:12750377). The GGPP
CC produced by the geranylgeranyl diphosphate synthase GGS2 is converted
CC to ent-kaurene via ent-copalyldiphosphate in a two-step cyclization
CC reaction performed by the bifunctional ent-copalyl diphosphate
CC synthase/ent-kaurene synthase enzyme (CPS/KS) (PubMed:9745028,
CC PubMed:10803977, PubMed:12750377). Ent-Kaurene is metabolized to GAs by
CC a series of oxidation reactions catalyzed by cytochrome P450
CC monooxygenases (PubMed:9917370, PubMed:12750377). Cytochrome P450
CC monooxygenase P450-4 is an ent-kaurene oxidase that catalyzes the three
CC oxidation steps between ent-kaurene and ent-kaurenoic acid
CC (PubMed:11472927). The highly multifunctional cytochrome P450
CC monooxygenase P450-1 then catalyzes four steps involving oxidation at
CC two carbon atoms, in the main pathway from ent-kaurenoic acid to GA14
CC via GA12-aldehyde as well as producing kaurenolides and fujenoic acids
CC as by-products (PubMed:11320210). The cytochrome P450 monooxygenase
CC P450-2 then converts GA14 to GA4 by removal of C-20 (PubMed:11943776).
CC GA4 is further converted to GA7 by the GA4 desaturase DES via 1,2-
CC desaturation before cytochrome P450 monooxygenase P450-3, a 13-
CC hydroxylase, hydroxylates GA7 to GA3, the final product of the GA-
CC biosynthetic pathway (PubMed:12750377). {ECO:0000269|PubMed:10347043,
CC ECO:0000269|PubMed:10803977, ECO:0000269|PubMed:11320210,
CC ECO:0000269|PubMed:11472927, ECO:0000269|PubMed:11943776,
CC ECO:0000269|PubMed:12750377, ECO:0000269|PubMed:15925394,
CC ECO:0000269|PubMed:23825955, ECO:0000269|PubMed:9745028,
CC ECO:0000269|PubMed:9917370}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC {ECO:0000269|PubMed:11320210, ECO:0000269|PubMed:9917370}.
CC -!- INDUCTION: Expression is induced under gibberellin-producing conditions
CC (PubMed:9917370). {ECO:0000269|PubMed:9917370}.
CC -!- DISRUPTION PHENOTYPE: Leads to the loss of gibberellins production
CC accumulates ent-kaurenoic acid but not later intermediates
CC (PubMed:9917370, PubMed:11320210). {ECO:0000269|PubMed:11320210,
CC ECO:0000269|PubMed:9917370}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; HF679027; CCT69174.1; -; Genomic_DNA.
DR AlphaFoldDB; S0E2U7; -.
DR SMR; S0E2U7; -.
DR EnsemblFungi; CCT69174; CCT69174; FFUJ_14333.
DR VEuPathDB; FungiDB:FFUJ_14333; -.
DR HOGENOM; CLU_022195_0_3_1; -.
DR UniPathway; UPA00390; -.
DR Proteomes; UP000016800; Chromosome 5.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0009686; P:gibberellin biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00465; EP450IV.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 1: Evidence at protein level;
KW Heme; Iron; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..440
FT /note="Cytochrome P450 monooygenase 1"
FT /id="PRO_0000442042"
FT BINDING 381
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
SQ SEQUENCE 440 AA; 50576 MW; C533DA9FA39CF91F CRC64;
MSPDKPFRIM GDVGELHILP PKYAYEVRNN EKLSFTMAAF KWFYAHLPGF EGFREGTNES
HIMKLVARHQ LTHQLTLVTG AVSEECALVL KDVYTDSPEW HDITAKDANM KLMARITSRV
FLGKEMCRNP QWLRITSTYA VIAFRAVEEL RLWPSWLRPV VQWFMPHCTQ SRALVQEARD
LINPLLERRR EEKAEAERTG EKVTYNDAVE WLDDLAREKG VGYDPACAQL SLSVAALHST
TDFFTQVMFD IAQNPELIEP LREEIIAVLG KQGWSKNSLY NLKLMDSVLK ESQRLKPIAI
ASMRRFTTHN VKLSDGVILP KNKLTLVSAH QHWDPEYYKD PLKFDGYRFF NMRREPGKES
KAQLVSATPD HMGFGYGLHA CPGRFFASEE IKIALSHILL KYDFKPVEGS SMEPRKYGLN
MNANPTAKLS VRRRKEEIAI
