GA45A_MOUSE
ID GA45A_MOUSE Reviewed; 165 AA.
AC P48316;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Growth arrest and DNA damage-inducible protein GADD45 alpha;
DE AltName: Full=DNA damage-inducible transcript 1 protein;
DE Short=DDIT-1;
GN Name=Gadd45a; Synonyms=Ddit1, Gadd45;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=129/Sv; TISSUE=Liver;
RX PubMed=8139541; DOI=10.1128/mcb.14.4.2361-2371.1994;
RA Zhan Q., Lord K.A., Alamo I. Jr., Hollander M.C., Carrier F., Ron D.,
RA Kohn K.W., Hoffman B., Liebermann D.A., Fornace A.J. Jr.;
RT "The gadd and MyD genes define a novel set of mammalian genes encoding
RT acidic proteins that synergistically suppress cell growth.";
RL Mol. Cell. Biol. 14:2361-2371(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=129/Sv; TISSUE=Liver;
RX PubMed=7509226;
RA Alimzhanov M.B., Kuprash D.V., Turetskaya R.L., Osipovich O.A.,
RA Borodulina O.R., Osovskaia V.S., Chumakov R.M., Nedospasov S.A.;
RT "Cloning and characteristics of murine genes coding for the human GADD45
RT analog -- a protein induced in response to DNA damage.";
RL Dokl. Akad. Nauk 333:788-791(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH MAPK14, AND FUNCTION.
RX PubMed=15735649; DOI=10.1038/ni1176;
RA Salvador J.M., Mittelstadt P.R., Belova G.I., Fornace A.J. Jr.,
RA Ashwell J.D.;
RT "The autoimmune suppressor Gadd45alpha inhibits the T cell alternative p38
RT activation pathway.";
RL Nat. Immunol. 6:396-402(2005).
CC -!- FUNCTION: Might affect PCNA interaction with some CDK (cell division
CC protein kinase) complexes; stimulates DNA excision repair in vitro and
CC inhibits entry of cells into S phase. In T-cells, functions as a
CC regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity.
CC {ECO:0000269|PubMed:15735649}.
CC -!- SUBUNIT: Interacts with AURKA, GADD45GIP1 and PCNA (By similarity).
CC Interacts with MAPK14. {ECO:0000250, ECO:0000269|PubMed:15735649}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- INDUCTION: By various types of DNA damage and growth arrest.
CC -!- SIMILARITY: Belongs to the GADD45 family. {ECO:0000305}.
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DR EMBL; L28177; AAA37656.1; -; mRNA.
DR EMBL; U00937; AAC27351.1; -; Unassigned_DNA.
DR EMBL; BC011141; AAH11141.1; -; mRNA.
DR CCDS; CCDS20217.1; -.
DR PIR; B56535; B56535.
DR RefSeq; NP_031862.1; NM_007836.1.
DR AlphaFoldDB; P48316; -.
DR SMR; P48316; -.
DR BioGRID; 199077; 6.
DR IntAct; P48316; 1.
DR STRING; 10090.ENSMUSP00000044034; -.
DR PhosphoSitePlus; P48316; -.
DR PaxDb; P48316; -.
DR PRIDE; P48316; -.
DR ProteomicsDB; 265726; -.
DR Antibodypedia; 33411; 430 antibodies from 34 providers.
DR DNASU; 13197; -.
DR Ensembl; ENSMUST00000043098; ENSMUSP00000044034; ENSMUSG00000036390.
DR GeneID; 13197; -.
DR KEGG; mmu:13197; -.
DR UCSC; uc009cfc.1; mouse.
DR CTD; 1647; -.
DR MGI; MGI:107799; Gadd45a.
DR VEuPathDB; HostDB:ENSMUSG00000036390; -.
DR eggNOG; ENOG502RY8P; Eukaryota.
DR GeneTree; ENSGT00950000182964; -.
DR HOGENOM; CLU_118164_0_0_1; -.
DR InParanoid; P48316; -.
DR OMA; CLLVDFP; -.
DR OrthoDB; 1517160at2759; -.
DR PhylomeDB; P48316; -.
DR TreeFam; TF300196; -.
DR Reactome; R-MMU-6804114; TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
DR BioGRID-ORCS; 13197; 6 hits in 73 CRISPR screens.
DR PRO; PR:P48316; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; P48316; protein.
DR Bgee; ENSMUSG00000036390; Expressed in granulocyte and 261 other tissues.
DR ExpressionAtlas; P48316; baseline and differential.
DR Genevisible; P48316; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0019900; F:kinase binding; ISO:MGI.
DR GO; GO:1990841; F:promoter-specific chromatin binding; IDA:MGI.
DR GO; GO:0046982; F:protein heterodimerization activity; ISO:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR GO; GO:0047485; F:protein N-terminus binding; ISO:MGI.
DR GO; GO:0071479; P:cellular response to ionizing radiation; ISO:MGI.
DR GO; GO:0071260; P:cellular response to mechanical stimulus; IEA:Ensembl.
DR GO; GO:0007098; P:centrosome cycle; IGI:MGI.
DR GO; GO:0016525; P:negative regulation of angiogenesis; IMP:CACAO.
DR GO; GO:0043537; P:negative regulation of blood vessel endothelial cell migration; IMP:CACAO.
DR GO; GO:0033140; P:negative regulation of peptidyl-serine phosphorylation of STAT protein; IMP:CACAO.
DR GO; GO:0006469; P:negative regulation of protein kinase activity; IDA:MGI.
DR GO; GO:0071901; P:negative regulation of protein serine/threonine kinase activity; ISO:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0043065; P:positive regulation of apoptotic process; ISO:MGI.
DR GO; GO:0046330; P:positive regulation of JNK cascade; ISO:MGI.
DR GO; GO:1900745; P:positive regulation of p38MAPK cascade; ISO:MGI.
DR GO; GO:2000379; P:positive regulation of reactive oxygen species metabolic process; ISO:MGI.
DR GO; GO:0051726; P:regulation of cell cycle; IDA:MGI.
DR GO; GO:0042770; P:signal transduction in response to DNA damage; ISO:MGI.
DR Gene3D; 3.30.1330.30; -; 1.
DR InterPro; IPR024824; GADD45.
DR InterPro; IPR029064; L30e-like.
DR InterPro; IPR004038; Ribosomal_L7Ae/L30e/S12e/Gad45.
DR PANTHER; PTHR10411; PTHR10411; 1.
DR Pfam; PF01248; Ribosomal_L7Ae; 1.
DR SUPFAM; SSF55315; SSF55315; 1.
PE 1: Evidence at protein level;
KW Cell cycle; DNA damage; Growth arrest; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..165
FT /note="Growth arrest and DNA damage-inducible protein
FT GADD45 alpha"
FT /id="PRO_0000148332"
FT MOD_RES 2
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P24522"
SQ SEQUENCE 165 AA; 18339 MW; C96BAB354A84770F CRC64;
MTLEEFSAAE QKTERMDTVG DALEEVLSKA RSQRTITVGV YEAAKLLNVD PDNVVLCLLA
ADEDDDRDVA LQIHFTLIRA FCCENDINIL RVSNPGRLAE LLLLENDAGP AESGGAAQTP
DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPER
