ID   GA45A_RAT               Reviewed;         165 AA.
AC   P48317;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Growth arrest and DNA damage-inducible protein GADD45 alpha;
DE   AltName: Full=DNA damage-inducible transcript 1 protein;
DE            Short=DDIT-1;
GN   Name=Gadd45a; Synonyms=Ddit1, Gadd45;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Wistar; TISSUE=Brain;
RX   PubMed=7828885; DOI=10.1016/0378-1119(94)90666-1;
RA   Yoshida T., Schneider E.L., Mori N.;
RT   "Cloning of the rat Gadd45 cDNA and its mRNA expression in the brain.";
RL   Gene 151:253-255(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8603754; DOI=10.1016/0014-5793(96)00012-9;
RA   Yoshida T., Okazaki T., Hughes P.E., Schneider E.L., Mori N.;
RT   "Cloning of rat GADD45 gene and induction analysis following ionizing
RT   radiation in vivo.";
RL   FEBS Lett. 380:87-92(1996).
CC   -!- FUNCTION: Might affect PCNA interaction with some CDK (cell division
CC       protein kinase) complexes; stimulates DNA excision repair in vitro and
CC       inhibits entry of cells into S phase. In T-cells, functions as a
CC       regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with AURKA, PCNA, GADD45GIP1 and MAPK14.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- INDUCTION: By various types of DNA damage and growth arrest.
CC   -!- SIMILARITY: Belongs to the GADD45 family. {ECO:0000305}.
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DR   EMBL; L32591; AAA96332.1; -; mRNA.
DR   EMBL; L39010; AAB02030.1; -; Genomic_DNA.
DR   PIR; S68690; S68690.
DR   AlphaFoldDB; P48317; -.
DR   SMR; P48317; -.
DR   STRING; 10116.ENSRNOP00000007698; -.
DR   PhosphoSitePlus; P48317; -.
DR   PaxDb; P48317; -.
DR   UCSC; RGD:2654; rat.
DR   RGD; 2654; Gadd45a.
DR   eggNOG; ENOG502RY8P; Eukaryota.
DR   InParanoid; P48317; -.
DR   PhylomeDB; P48317; -.
DR   Reactome; R-RNO-6804114; TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
DR   PRO; PR:P48317; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0019900; F:kinase binding; ISO:RGD.
DR   GO; GO:1990841; F:promoter-specific chromatin binding; ISO:RGD.
DR   GO; GO:0046982; F:protein heterodimerization activity; ISO:RGD.
DR   GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR   GO; GO:0047485; F:protein N-terminus binding; ISO:RGD.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; TAS:RGD.
DR   GO; GO:0071479; P:cellular response to ionizing radiation; ISO:RGD.
DR   GO; GO:0071260; P:cellular response to mechanical stimulus; ISO:RGD.
DR   GO; GO:0007098; P:centrosome cycle; ISO:RGD.
DR   GO; GO:0000086; P:G2/M transition of mitotic cell cycle; IEP:RGD.
DR   GO; GO:0016525; P:negative regulation of angiogenesis; ISO:RGD.
DR   GO; GO:0043537; P:negative regulation of blood vessel endothelial cell migration; ISO:RGD.
DR   GO; GO:0033140; P:negative regulation of peptidyl-serine phosphorylation of STAT protein; ISO:RGD.
DR   GO; GO:0006469; P:negative regulation of protein kinase activity; ISO:RGD.
DR   GO; GO:0071901; P:negative regulation of protein serine/threonine kinase activity; ISO:RGD.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR   GO; GO:0043065; P:positive regulation of apoptotic process; ISO:RGD.
DR   GO; GO:0046330; P:positive regulation of JNK cascade; ISO:RGD.
DR   GO; GO:1900745; P:positive regulation of p38MAPK cascade; ISO:RGD.
DR   GO; GO:2000379; P:positive regulation of reactive oxygen species metabolic process; ISO:RGD.
DR   GO; GO:0051726; P:regulation of cell cycle; ISO:RGD.
DR   GO; GO:0042770; P:signal transduction in response to DNA damage; ISO:RGD.
DR   Gene3D; 3.30.1330.30; -; 1.
DR   InterPro; IPR024824; GADD45.
DR   InterPro; IPR029064; L30e-like.
DR   InterPro; IPR004038; Ribosomal_L7Ae/L30e/S12e/Gad45.
DR   PANTHER; PTHR10411; PTHR10411; 1.
DR   Pfam; PF01248; Ribosomal_L7Ae; 1.
DR   SUPFAM; SSF55315; SSF55315; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; DNA damage; Growth arrest; Nucleus; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..165
FT                   /note="Growth arrest and DNA damage-inducible protein
FT                   GADD45 alpha"
FT                   /id="PRO_0000148333"
FT   MOD_RES         2
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P24522"
SQ   SEQUENCE   165 AA;  18480 MW;  6FB2956D27BE6BDE CRC64;
     MTLEEFSAAE QKIERMDTVG DALEEVLSKA RSQRTITVGV YEAAKLLNVD PDNVVLCLLA
     ADEDDDRDVA LQIHFTLIRA FCCENDINIL RVSNPGRLAE LLLLENDKSP AESGGLAQTP
     DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPER