GAA1_SCHPO
ID GAA1_SCHPO Reviewed; 581 AA.
AC Q9US48;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=GPI transamidase component gaa1;
GN Name=gaa1; ORFNames=SPAC1002.11;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Component of the GPI transamidase complex. Required for a
CC terminal step of GPI anchor attachment onto proteins (By similarity).
CC {ECO:0000250}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:16823372}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16823372}.
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DR EMBL; CU329670; CAB65611.1; -; Genomic_DNA.
DR RefSeq; NP_593498.1; NM_001018932.2.
DR AlphaFoldDB; Q9US48; -.
DR SMR; Q9US48; -.
DR STRING; 4896.SPAC1002.11.1; -.
DR PaxDb; Q9US48; -.
DR EnsemblFungi; SPAC1002.11.1; SPAC1002.11.1:pep; SPAC1002.11.
DR GeneID; 2543268; -.
DR KEGG; spo:SPAC1002.11; -.
DR PomBase; SPAC1002.11; gaa1.
DR VEuPathDB; FungiDB:SPAC1002.11; -.
DR eggNOG; KOG3566; Eukaryota.
DR HOGENOM; CLU_007442_3_1_1; -.
DR InParanoid; Q9US48; -.
DR OMA; RESEWNI; -.
DR PhylomeDB; Q9US48; -.
DR UniPathway; UPA00196; -.
DR PRO; PR:Q9US48; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:PomBase.
DR GO; GO:0042765; C:GPI-anchor transamidase complex; ISO:PomBase.
DR GO; GO:0016255; P:attachment of GPI anchor to protein; ISO:PomBase.
DR InterPro; IPR007246; Gaa1.
DR PANTHER; PTHR13304; PTHR13304; 1.
DR Pfam; PF04114; Gaa1; 1.
DR PIRSF; PIRSF036762; GAA1; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; GPI-anchor biosynthesis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..581
FT /note="GPI transamidase component gaa1"
FT /id="PRO_0000316598"
FT TOPO_DOM 1..17
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 39..366
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 388..403
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 404..424
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 425..427
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 428..448
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 449..467
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..488
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 489..500
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 501..521
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 522..548
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 549..569
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 570..581
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT MOTIF 577..581
FT /note="Prevents secretion from ER"
FT /evidence="ECO:0000255"
SQ SEQUENCE 581 AA; 66877 MW; 8B2BE07F2D605047 CRC64;
MSLFTFVQIR VFPFLQRHLF FLQLSLTLIG LSWIFILPRN EIIDRLHVSE SALLPGQVNT
YFENRYSKTV SSSLTAANTW SHLDASVGTN TMYDDLEQIF TAMGLPTQKQ NYSINIPGSE
FNGSNFITTL RAPRGDATES LLLCVPWKDH IGQYNEAGVA LAISLLKYFQ GWSLWSKDII
LVIFDDPVYG PSSFLTSYFD QTTPYISYTP LKIRSGSIQA GLSLELVTTE NNSDVLEVLY
QATNGQLPNL DLFNTISRIF MQHFNYPLRL QGYDFHANSG SSYTSRLKSL WMGMLTQAVS
NVTSAHALFP QYRIDMLTLR MKVKDPFSFD MFRFGQAIES TFRSLNNLLE HLHQSFFFYF
ILDHLHFISI GNYMPSILIL AASFMLGAYR HWINHEKKID LWRPFSFWLF SIFCTIAAYY
LVSSSTKITV FIFLYLMLTF IGIIFSTFMT SEDAELVLSY DLMSKSLFIS VVSTLNFSLS
FVVAILLVPL QFISFRFNRR LSLLFAVLTY FSTFIFLCSL SKILNGPLVP FWLWAKEYEL
FNSWLMPSVF MILVLPEIIF SVTSFFSLWN EPSVKTKTKT L
