ID   GAAP_HALAI              Reviewed;         507 AA.
AC   P86732;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Glycine, alanine and asparagine-rich protein;
DE   Flags: Precursor;
OS   Haliotis asinina (Donkey's ear abalone) (Ass's ear abalone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Vetigastropoda; Lepetellida; Haliotoidea; Haliotidae; Haliotis.
OX   NCBI_TaxID=109174;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION.
RC   TISSUE=Mantle {ECO:0000269|PubMed:19915030};
RX   PubMed=19915030; DOI=10.1093/molbev/msp278;
RA   Jackson D.J., McDougall C., Woodcroft B., Moase P., Rose R.A., Kube M.,
RA   Reinhardt R., Rokhsar D.S., Montagnani C., Joubert C., Piquemal D.,
RA   Degnan B.M.;
RT   "Parallel evolution of nacre building gene sets in molluscs.";
RL   Mol. Biol. Evol. 27:591-608(2010).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 42-73; 286-293; 296-303; 330-339 AND 346-355,
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Shell {ECO:0000269|PubMed:21050442};
RX   PubMed=21050442; DOI=10.1186/1477-5956-8-54;
RA   Marie B., Marie A., Jackson D.J., Dubost L., Degnan B.M., Milet C.,
RA   Marin F.;
RT   "Proteomic analysis of the organic matrix of the abalone Haliotis asinina
RT   calcified shell.";
RL   Proteome Sci. 8:54-54(2010).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21050442}.
CC   -!- TISSUE SPECIFICITY: Component of the acid-soluble and acid-insoluble
CC       organic matrix of calcified shell layers (at protein level).
CC       {ECO:0000269|PubMed:21050442}.
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DR   EMBL; GT271728; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT272311; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT272436; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT272926; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT273587; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT274163; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT274619; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT274653; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT274865; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT275005; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT275007; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT275313; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT276380; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT276151; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT277518; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GT277720; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; EZ420619; -; NOT_ANNOTATED_CDS; mRNA.
DR   AlphaFoldDB; P86732; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Coiled coil; Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..507
FT                   /note="Glycine, alanine and asparagine-rich protein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000399443"
FT   REGION          462..507
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          158..185
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        462..488
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        26..27
FT                   /note="RN -> LY (in Ref. 1; GT275005)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        100..105
FT                   /note="SGGSGF -> NGNGGN (in Ref. 1; GT275313)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        102..106
FT                   /note="GSGFG -> SPGN (in Ref. 1; GT274653)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        139
FT                   /note="S -> I (in Ref. 1; GT275007)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        165
FT                   /note="A -> S (in Ref. 1; GT274653)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        178
FT                   /note="Q -> H (in Ref. 1; GT274653)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        209..210
FT                   /note="EA -> KP (in Ref. 1; GT271728)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        300
FT                   /note="A -> S (in Ref. 1; GT276380)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        325..326
FT                   /note="SA -> FC (in Ref. 1; GT276151)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        332
FT                   /note="A -> T (in Ref. 1; GT276151)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        432
FT                   /note="G -> R (in Ref. 1; GT272926)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        462
FT                   /note="G -> R (in Ref. 1; GT277720)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        496
FT                   /note="G -> R (in Ref. 1; GT277518)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   507 AA;  45640 MW;  BD32956EE76DF96C CRC64;
     MLRVPLLVLC LALSVGADYY GYGWGRNGGG GGSGGGSGSS RASASASASA RANSIGNLVG
     RLTSLVDASA SARASASANA GGFGGSGAGG SGGNGFGGGS GGSGFGGGSG GSGFGGGSGG
     SGFGGGSGGS GFGGGSGGSG FGGGSGGSGF GGASASASAQ ALASATAELQ AAQDAYDQAS
     AYAEATARAA ANGGSLDSSA LASAIASAEA SVSARGASII ARARARAEAT VRAARRSFAS
     AQASAEASVS AVRSAEGRAR SFARAVARAR ASARAAIAGV RSSGRAFASA TARARASVSA
     AARAVARARA QAVARARASI RASASASARA SASAAAEARA AAYARVQVAA AAAARAAASA
     ASASASASAS GSSFGSGGSG GSGNGGFGSF GASANAVANA FAQAFGGGLG NGGNGGNGNG
     GNGGNGGNGN GGNGGNGNGG NGGNGNGGNG GNGGNGNGGN GGNGNGGNGR NGNGGNGRNG
     NGGNGGNGNG RNGRGGRYYY GSSDYYY