GAAP_VACCL
ID GAAP_VACCL Reviewed; 237 AA.
AC Q49P94;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 23-FEB-2022, entry version 41.
DE RecName: Full=Golgi anti-apoptotic protein;
DE Short=GAAP;
GN Name=L6; OrderedLocusNames=List196;
OS Vaccinia virus (strain Lister) (VACV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10252;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LC16m8, and LC16mO;
RX PubMed=16140764; DOI=10.1128/jvi.79.18.11873-11891.2005;
RA Morikawa S., Sakiyama T., Hasegawa H., Saijo M., Maeda A., Kurane I.,
RA Maeno G., Kimura J., Hirama C., Yoshida T., Asahi-Ozaki Y., Sata T.,
RA Kurata T., Kojima A.;
RT "An attenuated LC16m8 smallpox vaccine: analysis of full-genome sequence
RT and induction of immune protection.";
RL J. Virol. 79:11873-11891(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17554021; DOI=10.1099/vir.0.82708-0;
RA Garcel A., Crance J.M., Drillien R., Garin D., Favier A.L.;
RT "Genomic sequence of a clonal isolate of the vaccinia virus Lister strain
RT employed for smallpox vaccination in France and its comparison to other
RT orthopoxviruses.";
RL J. Gen. Virol. 88:1906-1916(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17319741; DOI=10.1371/journal.ppat.0030017;
RA Gubser C., Bergamaschi D., Hollinshead M., Lu X., van Kuppeveld F.J.,
RA Smith G.L.;
RT "A new inhibitor of apoptosis from vaccinia virus and eukaryotes.";
RL PLoS Pathog. 3:E17-E17(2007).
RN [4]
RP SUBCELLULAR LOCATION, AND TOPOLOGY.
RX PubMed=22418439; DOI=10.1074/jbc.m111.336149;
RA Carrara G., Saraiva N., Gubser C., Johnson B.F., Smith G.L.;
RT "Six-transmembrane topology for Golgi anti-apoptotic protein (GAAP) and Bax
RT inhibitor 1 (BI-1) provides model for the transmembrane Bax inhibitor-
RT containing motif (TMBIM) family.";
RL J. Biol. Chem. 287:15896-15905(2012).
CC -!- FUNCTION: May affect virulence through inhibition of apoptosis.
CC {ECO:0000269|PubMed:17319741}.
CC -!- SUBCELLULAR LOCATION: Host Golgi apparatus membrane
CC {ECO:0000305|PubMed:17319741, ECO:0000305|PubMed:22418439}; Multi-pass
CC membrane protein {ECO:0000305|PubMed:17319741,
CC ECO:0000305|PubMed:22418439}.
CC -!- SIMILARITY: Belongs to the BI1 family. LFG subfamily. {ECO:0000305}.
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DR EMBL; AY678275; AAW23650.1; -; Genomic_DNA.
DR EMBL; AY678277; AAW23932.1; -; Genomic_DNA.
DR EMBL; DQ121394; ABD52701.1; -; Genomic_DNA.
DR EMBL; AY699305; AAW21698.1; -; Genomic_DNA.
DR SMR; Q49P94; -.
DR GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR006214; Bax_inhibitor_1-related.
DR PANTHER; PTHR23291; PTHR23291; 1.
DR Pfam; PF01027; Bax1-I; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Host Golgi apparatus; Host membrane; Membrane; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..237
FT /note="Golgi anti-apoptotic protein"
FT /id="PRO_0000418100"
FT TOPO_DOM 1..37
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..67
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..94
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..174
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..209
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT INTRAMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 231..237
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 237 AA; 26244 MW; FE635453CEACB136 CRC64;
MAMPSLSACS SIEDDFNYGS SVASASVHIR MAFLRKVYGI LCLQFLLTTA TTAVFLYFDC
MRTFIQGSPV LILASMFGSI GLIFALTLHR HKHPLNLYLL CGFTLSESLT LASVVTFYDV
HVVMQAFMLT TAAFLALTTY TLQSKRDFSK LGAGLFAALW ILILSGLLGI FVQNETVKLV
LSAFGALVFC GFIIYDTHSL IHKLSPEEYV LASINLYLDI INLFLHLLQL LEVSNKK
