ID   GABBP_PSEAE             Reviewed;         352 AA.
AC   Q9I6R6;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Gamma-aminobutyric acid-binding protein {ECO:0000305};
DE            Short=GABA-binding protein {ECO:0000303|PubMed:31627455};
DE   Flags: Precursor;
GN   OrderedLocusNames=PA0222 {ECO:0000312|EMBL:AAG03611.1};
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION AS A BINDING PROTEIN.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=31627455; DOI=10.3390/ijms20205156;
RA   Fernandez M., Rico-Jimenez M., Ortega A., Daddaoua A., Garcia Garcia A.I.,
RA   Martin-Mora D., Torres N.M., Tajuelo A., Matilla M.A., Krell T.;
RT   "Determination of Ligand Profiles for Pseudomonas aeruginosa Solute Binding
RT   Proteins.";
RL   Int. J. Mol. Sci. 20:5156-5156(2019).
CC   -!- FUNCTION: Binds specifically gamma-aminobutyric acid (GABA) with
CC       nanomolar affinity. Does not bind structurally related compounds such
CC       as 4-aminovaleric acid, spermidine, histamine and butyric acid.
CC       {ECO:0000269|PubMed:31627455}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 1 family.
CC       {ECO:0000305}.
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DR   EMBL; AE004091; AAG03611.1; -; Genomic_DNA.
DR   PIR; F83617; F83617.
DR   RefSeq; NP_248913.1; NC_002516.2.
DR   RefSeq; WP_003101898.1; NZ_QZGE01000024.1.
DR   AlphaFoldDB; Q9I6R6; -.
DR   SMR; Q9I6R6; -.
DR   STRING; 287.DR97_3179; -.
DR   PaxDb; Q9I6R6; -.
DR   PRIDE; Q9I6R6; -.
DR   EnsemblBacteria; AAG03611; AAG03611; PA0222.
DR   GeneID; 877806; -.
DR   KEGG; pae:PA0222; -.
DR   PATRIC; fig|208964.12.peg.232; -.
DR   PseudoCAP; PA0222; -.
DR   HOGENOM; CLU_026974_8_0_6; -.
DR   InParanoid; Q9I6R6; -.
DR   OMA; MSWGGAY; -.
DR   PhylomeDB; Q9I6R6; -.
DR   BioCyc; PAER208964:G1FZ6-224-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006059; SBP.
DR   Pfam; PF13416; SBP_bac_8; 1.
PE   1: Evidence at protein level;
KW   Periplasm; Reference proteome; Signal; Transport.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..352
FT                   /note="Gamma-aminobutyric acid-binding protein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5004327156"
SQ   SEQUENCE   352 AA;  38472 MW;  E4C7B384278FCE53 CRC64;
     MFKSLHQYAH VFSRLSLFGL AFAAAAQAQS QSLTVISFGG ATKAAQEQAY FKPFERSGGG
     QVVAGEYNGE MAKVKAMVDV GKVSWDVVEV ESPELLRGCD EGLFERLDPA RFGDPAQFVP
     GTFSECGVAT YVWSMVMAYD STKLARAPQS WADFWNVREF PGKRGLRKGA KYTLEVALLA
     DGVKAEDLYK VLATPEGVSR AFAKLDQLKP NIQWWEAGAQ PPQWLAAGDV VMSAAYNGRI
     AAAQKEGVKL AIVWPGSLYD PEYWAVVKGT PNKALAEKFI AFASQPQTQK VFSEQIPYGP
     VHKGTLALLP KTVQEALPTA PANLEGARAV DAEFWVDHGE ELEQRFNAWA AR