GABPA_HUMAN
ID GABPA_HUMAN Reviewed; 454 AA.
AC Q06546; Q12939;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 206.
DE RecName: Full=GA-binding protein alpha chain;
DE Short=GABP subunit alpha;
DE AltName: Full=Nuclear respiratory factor 2 subunit alpha;
DE AltName: Full=Transcription factor E4TF1-60;
GN Name=GABPA; Synonyms=E4TF1A;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8441384; DOI=10.1128/mcb.13.3.1385-1391.1993;
RA Watanabe H., Sawada J., Yano K., Yamaguchi K., Goto M., Handa H.;
RT "cDNA cloning of transcription factor E4TF1 subunits with Ets and notch
RT motifs.";
RL Mol. Cell. Biol. 13:1385-1391(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7799916; DOI=10.1128/mcb.15.1.102;
RA Gugneja S., Virbasius J.V., Scarpulla R.C.;
RT "Four structurally distinct, non-DNA-binding subunits of human nuclear
RT respiratory factor 2 share a conserved transcriptional activation domain.";
RL Mol. Cell. Biol. 15:102-111(1995).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Transcription factor capable of interacting with purine rich
CC repeats (GA repeats). Necessary for the expression of the Adenovirus E4
CC gene.
CC -!- SUBUNIT: Heterotetramer of two alpha and two beta subunits.
CC -!- INTERACTION:
CC Q06546; Q06547: GABPB1; NbExp=3; IntAct=EBI-638925, EBI-618165;
CC Q06546; Q8TAK5: GABPB2; NbExp=4; IntAct=EBI-638925, EBI-8468945;
CC Q06546; P51610: HCFC1; NbExp=2; IntAct=EBI-638925, EBI-396176;
CC Q06546; O75716: STK16; NbExp=3; IntAct=EBI-638925, EBI-749295;
CC Q06546; Q5TFG8: ZC2HC1B; NbExp=3; IntAct=EBI-638925, EBI-12275374;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
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DR EMBL; D13318; BAA02575.1; -; mRNA.
DR EMBL; U13044; AAA65706.1; -; mRNA.
DR CCDS; CCDS13575.1; -.
DR PIR; A48146; A48146.
DR PIR; I38739; I38739.
DR RefSeq; NP_001184226.1; NM_001197297.1.
DR RefSeq; NP_002031.2; NM_002040.3.
DR RefSeq; XP_005260995.1; XM_005260938.4.
DR RefSeq; XP_011527822.1; XM_011529520.2.
DR RefSeq; XP_011527823.1; XM_011529521.2.
DR RefSeq; XP_016883802.1; XM_017028313.1.
DR AlphaFoldDB; Q06546; -.
DR BMRB; Q06546; -.
DR SMR; Q06546; -.
DR BioGRID; 108826; 115.
DR DIP; DIP-33980N; -.
DR IntAct; Q06546; 26.
DR MINT; Q06546; -.
DR STRING; 9606.ENSP00000346886; -.
DR GlyGen; Q06546; 4 sites, 2 O-linked glycans (4 sites).
DR iPTMnet; Q06546; -.
DR PhosphoSitePlus; Q06546; -.
DR BioMuta; GABPA; -.
DR DMDM; 729553; -.
DR EPD; Q06546; -.
DR jPOST; Q06546; -.
DR MassIVE; Q06546; -.
DR MaxQB; Q06546; -.
DR PaxDb; Q06546; -.
DR PeptideAtlas; Q06546; -.
DR PRIDE; Q06546; -.
DR ProteomicsDB; 58457; -.
DR Antibodypedia; 919; 416 antibodies from 32 providers.
DR DNASU; 2551; -.
DR Ensembl; ENST00000354828.7; ENSP00000346886.3; ENSG00000154727.11.
DR Ensembl; ENST00000400075.4; ENSP00000382948.3; ENSG00000154727.11.
DR GeneID; 2551; -.
DR KEGG; hsa:2551; -.
DR MANE-Select; ENST00000400075.4; ENSP00000382948.3; NM_002040.4; NP_002031.2.
DR UCSC; uc002ylx.5; human.
DR CTD; 2551; -.
DR DisGeNET; 2551; -.
DR GeneCards; GABPA; -.
DR HGNC; HGNC:4071; GABPA.
DR HPA; ENSG00000154727; Low tissue specificity.
DR MIM; 600609; gene.
DR neXtProt; NX_Q06546; -.
DR OpenTargets; ENSG00000154727; -.
DR PharmGKB; PA28485; -.
DR VEuPathDB; HostDB:ENSG00000154727; -.
DR eggNOG; KOG3806; Eukaryota.
DR GeneTree; ENSGT00940000155799; -.
DR HOGENOM; CLU_037064_0_0_1; -.
DR InParanoid; Q06546; -.
DR OMA; YSFWLQD; -.
DR OrthoDB; 526256at2759; -.
DR PhylomeDB; Q06546; -.
DR TreeFam; TF350537; -.
DR PathwayCommons; Q06546; -.
DR Reactome; R-HSA-2151201; Transcriptional activation of mitochondrial biogenesis.
DR SignaLink; Q06546; -.
DR BioGRID-ORCS; 2551; 523 hits in 1078 CRISPR screens.
DR ChiTaRS; GABPA; human.
DR GeneWiki; GABPA; -.
DR GenomeRNAi; 2551; -.
DR Pharos; Q06546; Tbio.
DR PRO; PR:Q06546; -.
DR Proteomes; UP000005640; Chromosome 21.
DR RNAct; Q06546; protein.
DR Bgee; ENSG00000154727; Expressed in calcaneal tendon and 186 other tissues.
DR ExpressionAtlas; Q06546; baseline and differential.
DR Genevisible; Q06546; HS.
DR GO; GO:0000785; C:chromatin; IDA:BHF-UCL.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IEA:Ensembl.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:ProtInc.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:MGI.
DR GO; GO:0001825; P:blastocyst formation; IEA:Ensembl.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:1903351; P:cellular response to dopamine; IMP:CAFA.
DR GO; GO:0045653; P:negative regulation of megakaryocyte differentiation; IEA:Ensembl.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:CAFA.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR024668; GABP_asu_N.
DR InterPro; IPR003118; Pointed_dom.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR016312; TF_GA-bd_asu.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849; PTHR11849; 1.
DR Pfam; PF00178; Ets; 1.
DR Pfam; PF11620; GABP-alpha; 1.
DR Pfam; PF02198; SAM_PNT; 1.
DR PIRSF; PIRSF001703; GABP_alpha; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SMART; SM00251; SAM_PNT; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
DR PROSITE; PS51433; PNT; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..454
FT /note="GA-binding protein alpha chain"
FT /id="PRO_0000204127"
FT DOMAIN 168..251
FT /note="PNT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00762"
FT DNA_BIND 320..400
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT REGION 297..316
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 303
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VARIANT 291
FT /note="A -> V (in dbSNP:rs2829897)"
FT /id="VAR_020315"
FT VARIANT 345
FT /note="E -> K (in dbSNP:rs2829900)"
FT /id="VAR_020316"
FT CONFLICT 289..290
FT /note="SS -> RC (in Ref. 2; AAA65706)"
FT /evidence="ECO:0000305"
FT CONFLICT 440
FT /note="A -> V (in Ref. 2; AAA65706)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 454 AA; 51295 MW; 1AF2ABBBC79191DD CRC64;
MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYAPAECVSQ AIDINEPIGN LKKLLEPRLQ
CSLDAHEICL QDIQLDPERS LFDQGVKTDG TVQLSVQVIS YQGIEPKLNI LEIVKPADTV
EVVIDPDAHH AESEAHLVEE AQVITLDGTK HITTISDETS EQVTRWAAAL EGYRKEQERL
GIPYDPIQWS TDQVLHWVVW VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL
WSHLELLRKY VLASQEQQMN EIVTIDQPVQ IIPASVQSAT PTTIKVINSS AKAAKVQRAP
RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL NQPELVAQKW
GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV CDLKTLIGYS AAELNRLVTE
CEQKKLAKMQ LHGIAQPVTA VALATASLQT EKDN
