GABPA_MOUSE
ID GABPA_MOUSE Reviewed; 454 AA.
AC Q00422; Q7TT22;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 194.
DE RecName: Full=GA-binding protein alpha chain;
DE Short=GABP subunit alpha;
GN Name=Gabpa; Synonyms=E4tf1a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1876836; DOI=10.1126/science.1876836;
RA Lamarco K., Thompson C.C., Byers B.P., Walton E.M., McKnight S.L.;
RT "Identification of Ets- and notch-related subunits in GA binding protein.";
RL Science 253:789-792(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, Egg, and Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 320-320.
RX PubMed=9461436; DOI=10.1126/science.279.5353.1037;
RA Batchelor A.H., Piper D.E., de la Brousse F.C., McKnight S.L.,
RA Wolberger C.;
RT "The structure of GABPalpha/beta: an ETS domain-ankyrin repeat heterodimer
RT bound to DNA.";
RL Science 279:1037-1041(1998).
CC -!- FUNCTION: Transcription factor capable of interacting with purine rich
CC repeats (GA repeats).
CC -!- SUBUNIT: Heterotetramer of two alpha and two beta subunits.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
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DR EMBL; M74515; AAA53030.1; -; mRNA.
DR EMBL; AK139916; BAE24181.1; -; mRNA.
DR EMBL; AK145446; BAE26442.1; -; mRNA.
DR EMBL; AK145838; BAE26687.1; -; mRNA.
DR EMBL; AK148087; BAE28337.1; -; mRNA.
DR EMBL; AK150455; BAE29575.1; -; mRNA.
DR EMBL; CH466521; EDK98317.1; -; Genomic_DNA.
DR EMBL; BC052448; AAH52448.1; -; mRNA.
DR CCDS; CCDS28284.1; -.
DR PIR; A40858; A40858.
DR RefSeq; NP_032091.2; NM_008065.2.
DR RefSeq; XP_006522970.1; XM_006522907.2.
DR PDB; 1AWC; X-ray; 2.15 A; A=320-429.
DR PDB; 1SXD; NMR; -; A=168-254.
DR PDB; 2JUO; NMR; -; A=35-121.
DR PDBsum; 1AWC; -.
DR PDBsum; 1SXD; -.
DR PDBsum; 2JUO; -.
DR AlphaFoldDB; Q00422; -.
DR BMRB; Q00422; -.
DR SMR; Q00422; -.
DR BioGRID; 199795; 1.
DR DIP; DIP-156N; -.
DR MINT; Q00422; -.
DR STRING; 10090.ENSMUSP00000009120; -.
DR iPTMnet; Q00422; -.
DR PhosphoSitePlus; Q00422; -.
DR EPD; Q00422; -.
DR MaxQB; Q00422; -.
DR PaxDb; Q00422; -.
DR PeptideAtlas; Q00422; -.
DR PRIDE; Q00422; -.
DR ProteomicsDB; 273409; -.
DR Antibodypedia; 919; 416 antibodies from 32 providers.
DR DNASU; 14390; -.
DR Ensembl; ENSMUST00000009120; ENSMUSP00000009120; ENSMUSG00000008976.
DR Ensembl; ENSMUST00000114184; ENSMUSP00000109822; ENSMUSG00000008976.
DR GeneID; 14390; -.
DR KEGG; mmu:14390; -.
DR UCSC; uc007ztj.1; mouse.
DR CTD; 2551; -.
DR MGI; MGI:95610; Gabpa.
DR VEuPathDB; HostDB:ENSMUSG00000008976; -.
DR eggNOG; KOG3806; Eukaryota.
DR GeneTree; ENSGT00940000155799; -.
DR HOGENOM; CLU_037064_0_0_1; -.
DR InParanoid; Q00422; -.
DR OMA; YSFWLQD; -.
DR OrthoDB; 526256at2759; -.
DR PhylomeDB; Q00422; -.
DR TreeFam; TF350537; -.
DR Reactome; R-MMU-2151201; Transcriptional activation of mitochondrial biogenesis.
DR BioGRID-ORCS; 14390; 20 hits in 77 CRISPR screens.
DR ChiTaRS; Gabpa; mouse.
DR EvolutionaryTrace; Q00422; -.
DR PRO; PR:Q00422; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q00422; protein.
DR Bgee; ENSMUSG00000008976; Expressed in undifferentiated genital tubercle and 277 other tissues.
DR Genevisible; Q00422; MM.
DR GO; GO:0000785; C:chromatin; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISO:MGI.
DR GO; GO:0001825; P:blastocyst formation; IMP:MGI.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:1903351; P:cellular response to dopamine; ISO:MGI.
DR GO; GO:0001701; P:in utero embryonic development; IMP:MGI.
DR GO; GO:0045653; P:negative regulation of megakaryocyte differentiation; IMP:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:MGI.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR024668; GABP_asu_N.
DR InterPro; IPR003118; Pointed_dom.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR016312; TF_GA-bd_asu.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849; PTHR11849; 1.
DR Pfam; PF00178; Ets; 1.
DR Pfam; PF11620; GABP-alpha; 1.
DR Pfam; PF02198; SAM_PNT; 1.
DR PIRSF; PIRSF001703; GABP_alpha; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SMART; SM00251; SAM_PNT; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
DR PROSITE; PS51433; PNT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..454
FT /note="GA-binding protein alpha chain"
FT /id="PRO_0000204128"
FT DOMAIN 168..251
FT /note="PNT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00762"
FT DNA_BIND 320..400
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT REGION 295..316
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 303
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q06546"
FT CONFLICT 188
FT /note="H -> R (in Ref. 1; AAA53030)"
FT /evidence="ECO:0000305"
FT STRAND 36..47
FT /evidence="ECO:0007829|PDB:2JUO"
FT HELIX 48..50
FT /evidence="ECO:0007829|PDB:2JUO"
FT HELIX 51..54
FT /evidence="ECO:0007829|PDB:2JUO"
FT HELIX 56..59
FT /evidence="ECO:0007829|PDB:2JUO"
FT STRAND 67..70
FT /evidence="ECO:0007829|PDB:2JUO"
FT TURN 81..83
FT /evidence="ECO:0007829|PDB:2JUO"
FT STRAND 90..99
FT /evidence="ECO:0007829|PDB:2JUO"
FT STRAND 107..115
FT /evidence="ECO:0007829|PDB:2JUO"
FT HELIX 168..177
FT /evidence="ECO:0007829|PDB:1SXD"
FT TURN 178..180
FT /evidence="ECO:0007829|PDB:1SXD"
FT HELIX 186..188
FT /evidence="ECO:0007829|PDB:1SXD"
FT HELIX 191..204
FT /evidence="ECO:0007829|PDB:1SXD"
FT HELIX 213..215
FT /evidence="ECO:0007829|PDB:1SXD"
FT HELIX 219..224
FT /evidence="ECO:0007829|PDB:1SXD"
FT HELIX 227..233
FT /evidence="ECO:0007829|PDB:1SXD"
FT HELIX 238..249
FT /evidence="ECO:0007829|PDB:1SXD"
FT TURN 250..252
FT /evidence="ECO:0007829|PDB:1SXD"
FT HELIX 322..330
FT /evidence="ECO:0007829|PDB:1AWC"
FT TURN 333..338
FT /evidence="ECO:0007829|PDB:1AWC"
FT STRAND 339..341
FT /evidence="ECO:0007829|PDB:1AWC"
FT STRAND 343..349
FT /evidence="ECO:0007829|PDB:1AWC"
FT HELIX 353..364
FT /evidence="ECO:0007829|PDB:1AWC"
FT HELIX 371..379
FT /evidence="ECO:0007829|PDB:1AWC"
FT HELIX 380..383
FT /evidence="ECO:0007829|PDB:1AWC"
FT STRAND 384..389
FT /evidence="ECO:0007829|PDB:1AWC"
FT STRAND 396..399
FT /evidence="ECO:0007829|PDB:1AWC"
FT HELIX 403..406
FT /evidence="ECO:0007829|PDB:1AWC"
FT HELIX 411..428
FT /evidence="ECO:0007829|PDB:1AWC"
SQ SEQUENCE 454 AA; 51344 MW; 2CBC40B895563685 CRC64;
MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYTPAECVSQ AIDINEPIGN LKKLLEPRLQ
CSLDAHEICL QDIQLDPDRS LFDQGVKTDG TVQLSVQVIS YQGMEPKLNI LEIVKTAETV
EVVIDPDAHH AEAEAHLVEE AQVITLDGTK HITTISDETS EQVTRWAAAL EGYRKEQERL
GIPYDPIHWS TDQVLHWVVW VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL
WSHLELLRKY VLASQEQQMN EIVTIDQPVQ IIPASVPPAT PTTIKVINSS AKAAKVQRSP
RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL NQPELVAQKW
GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV CDLKTLIGYS AAELNRLVIE
CEQKKLARMQ LHGIAQPVTA VALAATSLQA DKEI
