GACA_PSEAE
ID GACA_PSEAE Reviewed; 214 AA.
AC Q51373; O06649;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Response regulator GacA;
DE AltName: Full=Global activator;
GN Name=gacA; OrderedLocusNames=PA2586;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RA Kinscherf T.L., Sharp K.R., Kitten T.O., Willis D.K.;
RL Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=9159518; DOI=10.1046/j.1365-2958.1997.3291701.x;
RA Reimmann C., Beyeler M., Latifi A., Winteler H., Foglino M., Lazdunski A.,
RA Haas D.;
RT "The global activator GacA of Pseudomonas aeruginosa PAO positively
RT controls the production of the autoinducer N-butyryl-homoserine lactone and
RT the formation of the virulence factors pyocyanin, cyanide, and lipase.";
RL Mol. Microbiol. 24:309-319(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Positively controls the production of the autoinducer N-
CC butyryl-homoserine lactone and the formation of the virulence factors
CC pyocyanine, cyanide, and lipase.
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DR EMBL; U27988; AAA68948.1; -; Genomic_DNA.
DR EMBL; U89528; AAC45328.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG05974.1; -; Genomic_DNA.
DR PIR; D83322; D83322.
DR RefSeq; NP_251276.1; NC_002516.2.
DR RefSeq; WP_003090351.1; NZ_QZGE01000008.1.
DR AlphaFoldDB; Q51373; -.
DR SMR; Q51373; -.
DR IntAct; Q51373; 1.
DR MINT; Q51373; -.
DR STRING; 287.DR97_5459; -.
DR PaxDb; Q51373; -.
DR PRIDE; Q51373; -.
DR EnsemblBacteria; AAG05974; AAG05974; PA2586.
DR GeneID; 880582; -.
DR KEGG; pae:PA2586; -.
DR PATRIC; fig|208964.12.peg.2706; -.
DR PseudoCAP; PA2586; -.
DR HOGENOM; CLU_000445_90_1_6; -.
DR InParanoid; Q51373; -.
DR OMA; HQGNANF; -.
DR PhylomeDB; Q51373; -.
DR BioCyc; PAER208964:G1FZ6-2625-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:1900233; P:positive regulation of single-species biofilm formation on inanimate substrate; IMP:PseudoCAP.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd06170; LuxR_C_like; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR Pfam; PF00196; GerE; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS00622; HTH_LUXR_1; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 3: Inferred from homology;
KW Activator; Autoinducer synthesis; DNA-binding; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation;
KW Two-component regulatory system; Virulence.
FT CHAIN 1..214
FT /note="Response regulator GacA"
FT /id="PRO_0000081287"
FT DOMAIN 3..119
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 143..208
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 167..186
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT MOD_RES 54
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 99
FT /note="Missing (in Ref. 1; AAA68948)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 214 AA; 23609 MW; FB8D60AE0A40BA52 CRC64;
MIKVLVVDDH DLVRTGITRM LADIEGLQVV GQADCGEDCL KLARELKPDV VLMDVKMPGI
GGLEATRKLL RSQPDIKVVV VTVCEEDPFP TRLMQAGAAG YMTKGAGLEE MVQAIRQVFA
GQRYISPQIA QQLALKSFQP QQHDSPFDSL SEREIQIALM IANCHKVQSI SDKLCLSPKT
VNTYRYRIFE KLSITSDVEL ALLAVRHGMV DAAS
