ID   GACA_PSEU2              Reviewed;         214 AA.
AC   Q52376; Q4ZSE1;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   25-MAY-2022, entry version 133.
DE   RecName: Full=Response regulator GacA;
DE   AltName: Full=Global activator;
GN   Name=gacA; OrderedLocusNames=Psyr_2897;
OS   Pseudomonas syringae pv. syringae (strain B728a).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=205918;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8002569; DOI=10.1128/jb.176.24.7468-7475.1994;
RA   Rich J.J., Kinscherf T.G., Kitten T., Willis D.K.;
RT   "Genetic evidence that the gacA gene encodes the cognate response regulator
RT   for the lemA sensor in Pseudomonas syringae.";
RL   J. Bacteriol. 176:7468-7475(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B728a;
RX   PubMed=16043691; DOI=10.1073/pnas.0504930102;
RA   Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A.,
RA   Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S.,
RA   Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M.,
RA   Kyrpides N.C., Ivanova N., Lindow S.E.;
RT   "Comparison of the complete genome sequences of Pseudomonas syringae pv.
RT   syringae B728a and pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005).
CC   -!- FUNCTION: Forms part of a two-component regulatory system
CC       GacA/GacA(LemA). May be involved in lesion formation, swarming and in
CC       the production of extracellular protease, syringomycin and N-acyl-L-
CC       homoserine lactone (acyl-HSL).
CC   -!- PTM: Phosphorylated by LemA. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA65231.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAY37931.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U09767; AAA65231.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP000075; AAY37931.1; ALT_INIT; Genomic_DNA.
DR   PIR; A55538; A55538.
DR   RefSeq; WP_002553344.1; NC_007005.1.
DR   RefSeq; YP_235969.1; NC_007005.1.
DR   AlphaFoldDB; Q52376; -.
DR   SMR; Q52376; -.
DR   STRING; 205918.Psyr_2897; -.
DR   EnsemblBacteria; AAY37931; AAY37931; Psyr_2897.
DR   GeneID; 61686344; -.
DR   GeneID; 64443367; -.
DR   GeneID; 65074266; -.
DR   KEGG; psb:Psyr_2897; -.
DR   PATRIC; fig|205918.7.peg.2953; -.
DR   eggNOG; COG2197; Bacteria.
DR   HOGENOM; CLU_000445_90_1_6; -.
DR   Proteomes; UP000000426; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   Pfam; PF00196; GerE; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS00622; HTH_LUXR_1; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Phosphoprotein; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..214
FT                   /note="Response regulator GacA"
FT                   /id="PRO_0000081289"
FT   DOMAIN          3..119
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          143..208
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        167..186
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   MOD_RES         54
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   214 AA;  23434 MW;  1FDCCD9DAD07869C CRC64;
     MIKVLVVDDH DLVRTGITRM LADIDGLQVV GQADSGEESL KKARELKPDV VLMDVKMPGI
     GGLEATRKML RSHPDIKVVA VTVCEEDPFP TRLLQAGAAG YMTKGAGLAE MVQAIRLVFA
     GQRYISPQIA QQLALKSFQP QVNNSPFDLL SEREIQIALM IVGCQKVQTI SDKLCLSPKT
     VNTYRYRIFE KLSISSDVEL ALLAVRHGMV DASA