ID   ALG8_AZOVI              Reviewed;         492 AA.
AC   P94199;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Glycosyltransferase alg8;
DE            EC=2.4.-.-;
GN   Name=alg8;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 9046;
RX   PubMed=9358065; DOI=10.1016/s0378-1119(97)00380-6;
RA   Mejia-Ruiz H., Guzman J., Moreno S., Soberon-Chavez G., Espin G.;
RT   "The Azotobacter vinelandii alg8 and alg44 genes are essential for alginate
RT   synthesis and can be transcribed from an algD-independent promoter.";
RL   Gene 199:271-277(1997).
CC   -!- FUNCTION: Possibly a processive enzyme that polymerizes GDP-mannuronic
CC       acid.
CC   -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR   EMBL; Y08819; CAA70053.1; -; Genomic_DNA.
DR   AlphaFoldDB; P94199; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   UniPathway; UPA00286; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Alginate biosynthesis; Cell membrane; Glycosyltransferase; Membrane;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..492
FT                   /note="Glycosyltransferase alg8"
FT                   /id="PRO_0000059253"
FT   TRANSMEM        13..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        47..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        379..401
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        421..443
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   492 AA;  56284 MW;  9E54A4733CD1BE14 CRC64;
     MDRLKHALGE ATGWLLFLSF LMLVAVALPP QVFDPESKHF IMLIGLIGVW RYSMGIIHFL
     RGMLFLYVVY PYYRRKVEKL GKDADPSHVF LMVTSFRIDA LTTGKVYGSV IKEAINCGYP
     TTVVCSIVEM SDELLIKSLW EKLDPPDRVK LDFVRIAGTG KRDGLANGFR AISRHMPDED
     AVVAVIDGDT VLNEGVVRKT VPLFQDLPQH GWPDHQRILR SAGRLRHERV AQVRFAQRHI
     NMCSMALSHR VLTLTGRMSV FRAVVTDPEF IVDVENDNLD HWRLGRFKFL TGDDKSSWFS
     LMRLGYDTFY VPDASIHTVE HPPEKRFVKA SRKLMFRWYG NNLRQNSRAL KLGVQRLGWF
     TSVVLFDQRV SMWTSLLGLT VAIIGSIKYS IAIFIAYLLW VCSTRLVLTL LLSLSGHPIG
     PAYPLILYYN QIVGAVVKIH VFFRLDQQSW TRQDTKLNRE LASFQSWFNN WSSKAMTFSA
     TSIFIAVLML SV