ID   GACD_DICDI              Reviewed;         514 AA.
AC   Q54F80;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Rho GTPase-activating protein gacD;
DE   AltName: Full=GTPase activating factor for raC protein D;
GN   Name=gacD; ORFNames=DDB_G0291021;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   INDUCTION [LARGE SCALE ANALYSIS].
RX   PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA   Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA   Ivens A., Martinez J.L., Escalante R.;
RT   "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT   and specific effects from PAO1 and PA14 strains.";
RL   BMC Microbiol. 8:109-109(2008).
CC   -!- FUNCTION: Rho GTPase-activating protein involved in the signal
CC       transduction pathway. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Down-regulated by Pseudomonas aeruginosa, PAO1 strain and
CC       PA14 strain infection. {ECO:0000269|PubMed:18590548}.
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DR   EMBL; AAFI02000174; EAL61941.1; -; Genomic_DNA.
DR   RefSeq; XP_635457.1; XM_630365.1.
DR   AlphaFoldDB; Q54F80; -.
DR   SMR; Q54F80; -.
DR   STRING; 44689.DDB0233876; -.
DR   PaxDb; Q54F80; -.
DR   EnsemblProtists; EAL61941; EAL61941; DDB_G0291021.
DR   GeneID; 8627957; -.
DR   KEGG; ddi:DDB_G0291021; -.
DR   dictyBase; DDB_G0291021; gacD.
DR   eggNOG; KOG1450; Eukaryota.
DR   HOGENOM; CLU_530459_0_0_1; -.
DR   InParanoid; Q54F80; -.
DR   OMA; GVCFAPN; -.
DR   PhylomeDB; Q54F80; -.
DR   PRO; PR:Q54F80; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   Gene3D; 1.10.555.10; -; 1.
DR   InterPro; IPR008936; Rho_GTPase_activation_prot.
DR   InterPro; IPR000198; RhoGAP_dom.
DR   Pfam; PF00620; RhoGAP; 1.
DR   SMART; SM00324; RhoGAP; 1.
DR   SUPFAM; SSF48350; SSF48350; 1.
DR   PROSITE; PS50238; RHOGAP; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; GTPase activation; Reference proteome.
FT   CHAIN           1..514
FT                   /note="Rho GTPase-activating protein gacD"
FT                   /id="PRO_0000380196"
FT   DOMAIN          185..369
FT                   /note="Rho-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT   REGION          1..46
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          61..91
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          105..156
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          401..446
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..21
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        105..141
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        401..421
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        422..446
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   514 AA;  56088 MW;  D2377D73C557CF3E CRC64;
     MSNPSKLSIS PPNTFQHVGG SNLHVNKPPA LQSPPRSPPL QHTTAQNNLA KSTSASNIAG
     IANQTTPSSS GSSSGSSSLN SIGSLTKSTS NSNLNINHYS SGNATMGASL QSTNTGNTIT
     QSSSPPSSSS STNNNNNVLP LPNKILHHPS PKKTTKSTII SGKLKRFFKS RPSRDSLYEK
     HILSAPFSSA TLSFINIFKI ITALKKTNAI ETEGLFRVNG NAESVRSLWL MLNNVQEAIP
     VDGNSSAHDL AGLLKLYLRE SKFPLVPLEL FTSPAVPATI DEARVFVEEK LPTENVRVLS
     YLIEYLEQVS LKSSINKMNP IGLGVCFAPN ILRSVNTNPS NPQFQVHMTN IQSHCNFITL
     LIENRLKIFK DSALSPVQGS NDDEILENLI IQSPDDELFD EQHQHEQELI DSSHEHHHSI
     NNGDNNNNVD TSNVNGGSGD ENNQSNVIND TALYDEFISL LTDESLGSSQ KSVIIQRMTT
     LFQTDGDRFK SFLRDMQIDG IISLLEYILT LDEN