GACFF_DICDI
ID GACFF_DICDI Reviewed; 885 AA.
AC Q86I31; Q554B1;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Rho GTPase-activating protein gacFF;
DE AltName: Full=GTPase activating factor for raC protein FF;
GN Name=gacFF; ORFNames=DDB_G0275085;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA Ivens A., Martinez J.L., Escalante R.;
RT "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT and specific effects from PAO1 and PA14 strains.";
RL BMC Microbiol. 8:109-109(2008).
CC -!- FUNCTION: Rho GTPase-activating protein involved in the signal
CC transduction pathway. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- INDUCTION: Up-regulated by Pseudomonas aeruginosa, PAO1 strain and PA14
CC strain infection. {ECO:0000269|PubMed:18590548}.
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DR EMBL; AAFI02000013; EAL69823.1; -; Genomic_DNA.
DR RefSeq; XP_643800.1; XM_638708.1.
DR AlphaFoldDB; Q86I31; -.
DR SMR; Q86I31; -.
DR STRING; 44689.DDB0233844; -.
DR PaxDb; Q86I31; -.
DR EnsemblProtists; EAL69823; EAL69823; DDB_G0275085.
DR GeneID; 8619845; -.
DR KEGG; ddi:DDB_G0275085; -.
DR dictyBase; DDB_G0275085; gacFF.
DR eggNOG; KOG0930; Eukaryota.
DR eggNOG; KOG4269; Eukaryota.
DR HOGENOM; CLU_325835_0_0_1; -.
DR InParanoid; Q86I31; -.
DR OMA; MPTLKCS; -.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR Reactome; R-DDI-9013423; RAC3 GTPase cycle.
DR Reactome; R-DDI-9013424; RHOV GTPase cycle.
DR PRO; PR:Q86I31; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0043087; P:regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF12937; F-box-like; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00256; FBOX; 1.
DR SMART; SM00233; PH; 2.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Cytoplasm; GTPase activation; Reference proteome.
FT CHAIN 1..885
FT /note="Rho GTPase-activating protein gacFF"
FT /id="PRO_0000380200"
FT DOMAIN 348..394
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT DOMAIN 464..571
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 701..885
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 168..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 594..680
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 222..249
FT /evidence="ECO:0000255"
FT COMPBIAS 594..657
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 666..680
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 885 AA; 99580 MW; 3BECD2C659F60D6D CRC64;
MNGSPYSSSS WVKSSLSTNS LGSLSNSYAA GSAGGSGNRS TVRGDSNKDQ KLLGYLQKQS
KSQLPNSNVK IFKKKWFWFE SDSGTLFYSA TSTGALSTPL TPSSLSENIK SIKITSQTTI
EQSISFRLEL IIKDKVPNDE FTVLNWVNTL NQWKKLNCNL DFTNDTTTTT NNSNNSNSNN
NNKQYNSIEN PDYLGKFGEV EGLLNAMSVS TSLNTMKTVK PLINKIQNDS EQLKLVLSQV
EQQIEFLKSC QLELISHLSN DNNNNNNNNN NNNNILQILQ QQQPISSKRT VSNDNLVGSN
NSNNQLSLSI GLPINLNTSN TTTTTITNNT IEGENDDDEE EDEINQTSDI FSLLPTHLTL
YVFSYLEPKE LLILAQVSSQ WQKLAGDNLL WVRFVTHFIT PASIFDKSHN WKSVYLANTV
TSLGYCSNYY YLKNQQKGGI CTNKEKYMNR AISMYGVTPL TSLSSSKEGW LYKRGDDLLR
IWKKRYFVLR DSSLFYFKHQ NDNFPCGVIL LNHGTKLKRA SASTRKNCFK ILQSKNSTIT
MVHKKRMPYY LSTDKEDDCN DWMILLNSII KSNTQHQNNY GVITISGTSN NNNNNVYINN
NNNNNNNNNN NNNNNNNNNN NNGSQSYNLP PLLSKSPSFS NALTSSTGIS GGGGGSTKSR
KSHKKSFSSG GGGSGGNNNF EQMQQQIQSV HLTPIYPMFG VALSKILENQ SLITTQTHLK
IPFILYICLN YIISKGIKEE GIFRVSGSLR EVQELQEHFE QGREIDLDQH DIHAICGLVK
TFFRKLPHTL VAADLDEYST SVQLAASQTE DEKIQEFKFI FESISENSFH IFELLLYCLR
LIVRNESFNK MTIENLLIVI MPTLKCSPVL ITNGIKYYDE IFIKK
