GACF_DICDI
ID GACF_DICDI Reviewed; 1140 AA.
AC Q8SSN9; Q555L0;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 2.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Rho GTPase-activating protein gacF;
DE AltName: Full=GTPase activating factor for raC protein F;
GN Name=gacF; ORFNames=DDB_G0274243;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Rho GTPase-activating protein involved in the signal
CC transduction pathway. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR EMBL; AAFI02000012; EAL70014.1; -; Genomic_DNA.
DR RefSeq; XP_644056.1; XM_638964.1.
DR AlphaFoldDB; Q8SSN9; -.
DR SMR; Q8SSN9; -.
DR STRING; 44689.DDB0233846; -.
DR PaxDb; Q8SSN9; -.
DR PRIDE; Q8SSN9; -.
DR EnsemblProtists; EAL70014; EAL70014; DDB_G0274243.
DR GeneID; 8619485; -.
DR KEGG; ddi:DDB_G0274243; -.
DR dictyBase; DDB_G0274243; gacF.
DR eggNOG; KOG3565; Eukaryota.
DR HOGENOM; CLU_277914_0_0_1; -.
DR InParanoid; Q8SSN9; -.
DR OMA; LEEYACI; -.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR Reactome; R-DDI-9013404; RAC2 GTPase cycle.
DR Reactome; R-DDI-9013406; RHOQ GTPase cycle.
DR Reactome; R-DDI-9013409; RHOJ GTPase cycle.
DR Reactome; R-DDI-9013423; RAC3 GTPase cycle.
DR PRO; PR:Q8SSN9; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IBA:GO_Central.
DR Gene3D; 1.10.555.10; -; 1.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 3: Inferred from homology;
KW Coiled coil; Cytoplasm; GTPase activation; Reference proteome.
FT CHAIN 1..1140
FT /note="Rho GTPase-activating protein gacF"
FT /id="PRO_0000380199"
FT DOMAIN 234..409
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 1..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 189..236
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 455..504
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 520..644
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 661..700
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 720..759
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 773..927
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 952..1095
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 28..55
FT /evidence="ECO:0000255"
FT COILED 517..548
FT /evidence="ECO:0000255"
FT COMPBIAS 12..139
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..223
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 520..534
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 549..595
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 610..644
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 668..690
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 773..873
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 880..927
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1140 AA; 124451 MW; 7B5FAF46D83DC4CC CRC64;
MKTHKKKKSL GGLFSHSSSS PNLKSFLTEE VIHEQQQQQQ QHNNNNNNNN NHQRQPSTTS
TSSYIDSASS SIEETSGYLS KTSSSSSLPS SPLHNQNQNQ NGNIQLNSSS GSLNNNEQQQ
QQQSISQTSS PNTSSPIMGK KKPSRLAQAF KRVKNNGNNK LKKEIEELTN KTGLNQQYTH
SNLPFNVVES DIDNGSSGGT TSSTGNIISH SKSPSSSSSS SSSKKHQRKS RFIEPISQST
EDYTDIPRII KMSVEYLFEK CLLVPGIFRE SANAMELQRL SQLFEKGGDI DLSEYTDPHC
IGGLLKLYFR EKPIPIFPYD LHKRIYSVLN EEDSNIKVKS LLESGLGKGQ FLILRYLFEL
LNAVSINCNV NFMNYQNLAI CFAPSLIQSF DLSCYDVIER LIENYHSIFG VVVPVVDNSS
NVSSSSLAHP IIGGKSETNV SLNNLTVTIL DHGDINNNSN SSNNNNSSSS SSPYKKSPKP
SPKSSPKLNN RNSISPKLSS STSFRKSINL SSNSIMISDE VQQEQQNQQQ QQDEQQDEQQ
DEQQDEQQDE QNSNSTSINT SSSSITRPRK GSTVQYLNRI NTCRRPSSWT NNNRIKQQQH
HHHHHQQQQQ HQQHQQQQSS SSESNSSLTS SPQKRLNSVN GLESYEEGKN VNEIYSSLKE
ESSKLPKKSS LNRQMTIVNS NNIGNGDEKN SDCTTSDEDE ELKISKPIIR STSVNEILKE
TNDDNNNNDQ INNSNSSNNI PKTTITTTTN NTTTTNNISP IVKSKSQIIT SSAKVTPTPT
PAPMQTSSFL STKQTNSPSS SSSPSSTVSS TSSSPSSSLS SSIDNKTMSN VNYNRFQPAN
RTVSSPNVRN FSVPTTTTTT SIGSNSFVSP RFIGKRTTPS IFSPRKQSIC KPKNTTSSLS
SSSSNISKST NSTPTPTPAP ILTSTLSVNS TSSSSKLQEK ALPTKTTFIT QTSSPLLLPQ
PLTSTSTSTS TSTSTSTSTS TSTSTTIPTP TPTTSSFKSN SFSTSNDNSN NNNNNNNNNN
NNNNNNNNNN NNNNNVTSPT IVSSPSSPTS PISPTSSTFI KGHSHSNSLS QTPSSSSSSL
GNRLTAPGRQ LNHTNSTSSL ISKFNQIDTP SGINQGWKGG VVVQKKQDPV KKSLAIFEKE
