ID   GACL_DICDI              Reviewed;         734 AA.
AC   Q54FF4;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Rho GTPase-activating protein gacL;
DE   AltName: Full=GTPase activating factor for raC protein L;
GN   Name=gacL; ORFNames=DDB_G0290891;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   INDUCTION [LARGE SCALE ANALYSIS].
RX   PubMed=18559084; DOI=10.1186/1471-2164-9-291;
RA   Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B.,
RA   Skelton J., Ivens A., Bozzaro S.;
RT   "Genome-wide transcriptional changes induced by phagocytosis or growth on
RT   bacteria in Dictyostelium.";
RL   BMC Genomics 9:291-291(2008).
CC   -!- FUNCTION: Rho GTPase-activating protein involved in the signal
CC       transduction pathway. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Down-regulated by phagocytic stimuli.
CC       {ECO:0000269|PubMed:18559084}.
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DR   EMBL; AAFI02000171; EAL62010.1; -; Genomic_DNA.
DR   RefSeq; XP_635517.1; XM_630425.1.
DR   AlphaFoldDB; Q54FF4; -.
DR   SMR; Q54FF4; -.
DR   STRING; 44689.DDB0233793; -.
DR   PaxDb; Q54FF4; -.
DR   PRIDE; Q54FF4; -.
DR   EnsemblProtists; EAL62010; EAL62010; DDB_G0290891.
DR   GeneID; 8627884; -.
DR   KEGG; ddi:DDB_G0290891; -.
DR   dictyBase; DDB_G0290891; gacL.
DR   eggNOG; KOG4270; Eukaryota.
DR   HOGENOM; CLU_377876_0_0_1; -.
DR   InParanoid; Q54FF4; -.
DR   OMA; WIGGDEG; -.
DR   Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR   Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR   Reactome; R-DDI-9013404; RAC2 GTPase cycle.
DR   Reactome; R-DDI-9013406; RHOQ GTPase cycle.
DR   Reactome; R-DDI-9013409; RHOJ GTPase cycle.
DR   Reactome; R-DDI-9013423; RAC3 GTPase cycle.
DR   PRO; PR:Q54FF4; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR   GO; GO:0007264; P:small GTPase mediated signal transduction; IBA:GO_Central.
DR   Gene3D; 1.10.555.10; -; 1.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR008936; Rho_GTPase_activation_prot.
DR   InterPro; IPR000198; RhoGAP_dom.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   Pfam; PF00620; RhoGAP; 1.
DR   SMART; SM00324; RhoGAP; 1.
DR   SUPFAM; SSF48350; SSF48350; 1.
DR   SUPFAM; SSF50998; SSF50998; 1.
DR   PROSITE; PS50238; RHOGAP; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; GTPase activation; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..734
FT                   /note="Rho GTPase-activating protein gacL"
FT                   /id="PRO_0000380210"
FT   DOMAIN          141..339
FT                   /note="Rho-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT   REPEAT          381..430
FT                   /note="WD 1"
FT   REPEAT          539..579
FT                   /note="WD 2"
FT   REPEAT          585..623
FT                   /note="WD 3"
SQ   SEQUENCE   734 AA;  81913 MW;  26DBE90E99DD7929 CRC64;
     MDIELFLSNY NIANGTSSTT ANNSKFQPKI KVKLVVESGS STYEKSLEST NDQLKPLIIK
     LNNKIKKYEE SLNKKNFVTP NKQSSSSMNL LQKSSTVNLS SPSLSQIGLT HHASLKNLQI
     TTSPDIQQPP SVPSNPIVFG ISLDTLIAKE KSMELRIPTF VSNILNTLFL HSLGVEGLFR
     ISGSQMEIQN RRGIVNLGDY STSRDDNPHV LTVLLKQFLR DLPEPICTNA LYDLFLAASD
     QINFQQCKEN GFEVLKKLIN SNLPVNNRNL LQHLIHFLRF VAMNQTVNLM GPSNLSRVFG
     PNLFWKKETG PLDIQILQST SEKVNFITEQ MILHYDTLFE EPLQTTSPAV LSNTPINSNP
     ASIPTFNFGG KKLVIHSKLL GHNKSIQWMT LCGSSNSNGN SELSGSRVWS LDSHGMARIW
     DSQNQQFIKE VEVVDQSKGG GGAVYQMIST TVNNTVWTAT SQKLAIWDID GGLIGEIPGE
     SYSLCESQNK EIWVGGPQVL NIYSLDGIPS IAPSNCTSDD NNSNGAGGGE LLKPIGTNLF
     MKGIIILAMC KVGQNRIWGC SSEKTLYVWD TKTKETIHQC EIQEKRPKRM ACIEIDDTEY
     IWIGGDEGSI QIFNSKTFKL EHKILNQGWD KIFHIASINR EIWIGFWDTT VRVFPGDPKN
     KEILIDYKGY HSDVVSSIIE VPNLKGGDPF VWFGSYDKSI SIVSIKEDTA KSWNFTNIKK
     NFTRPIRQGF SSRG