GACP4_ARATH
ID GACP4_ARATH Reviewed; 745 AA.
AC Q9M350; Q6NLC2;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 2.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Gamma-tubulin complex component 4;
DE Short=AtGCP4;
DE Short=GCP-4;
GN Name=GCP4; OrderedLocusNames=At3g53760; ORFNames=F5K20_60;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP INDUCTION BY NEMATODES.
RX PubMed=22144887; DOI=10.1371/journal.ppat.1002343;
RA Banora M.Y., Rodiuc N., Baldacci-Cresp F., Smertenko A., Bleve-Zacheo T.,
RA Mellilo M.T., Karimi M., Hilson P., Evrard J.L., Favery B., Engler G.,
RA Abad P., de Almeida Engler J.;
RT "Feeding cells induced by phytoparasitic nematodes require gamma-tubulin
RT ring complex for microtubule reorganization.";
RL PLoS Pathog. 7:E1002343-E1002343(2011).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=22427335; DOI=10.1105/tpc.111.094904;
RA Janski N., Masoud K., Batzenschlager M., Herzog E., Evrard J.L., Houlne G.,
RA Bourge M., Chaboute M.E., Schmit A.C.;
RT "The GCP3-interacting proteins GIP1 and GIP2 are required for gamma-tubulin
RT complex protein localization, spindle integrity, and chromosomal
RT stability.";
RL Plant Cell 24:1171-1187(2012).
CC -!- FUNCTION: Gamma-tubulin complex is necessary for microtubule nucleation
CC at the microtubule organizing centers (MTOCs). {ECO:0000250}.
CC -!- FUNCTION: Gamma-tubulin complex is essential for the control of
CC microtubular network remodeling in the course of initiation and
CC development of giant-feeding cells, and for the successful reproduction
CC of nematodes (e.g. Meloidogyne spp.) in their plant hosts (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Gamma-tubulin complex is composed of gamma-tubulin and GCP
CC proteins.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center {ECO:0000250|UniProtKB:Q9BSJ2}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:22427335}.
CC -!- INDUCTION: Up-regulated in galls upon nematode infection.
CC {ECO:0000269|PubMed:22144887}.
CC -!- SIMILARITY: Belongs to the TUBGCP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB88338.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL132960; CAB88338.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79139.1; -; Genomic_DNA.
DR EMBL; BT012227; AAS76714.1; -; mRNA.
DR EMBL; BT012412; AAS92328.1; -; mRNA.
DR PIR; T45916; T45916.
DR RefSeq; NP_190944.2; NM_115236.4.
DR AlphaFoldDB; Q9M350; -.
DR SMR; Q9M350; -.
DR BioGRID; 9860; 3.
DR IntAct; Q9M350; 1.
DR STRING; 3702.AT3G53760.1; -.
DR PaxDb; Q9M350; -.
DR PRIDE; Q9M350; -.
DR ProteomicsDB; 247385; -.
DR EnsemblPlants; AT3G53760.1; AT3G53760.1; AT3G53760.
DR GeneID; 824543; -.
DR Gramene; AT3G53760.1; AT3G53760.1; AT3G53760.
DR KEGG; ath:AT3G53760; -.
DR Araport; AT3G53760; -.
DR TAIR; locus:2084430; AT3G53760.
DR eggNOG; KOG2065; Eukaryota.
DR HOGENOM; CLU_012029_0_0_1; -.
DR InParanoid; Q9M350; -.
DR OMA; WAAVMHQ; -.
DR OrthoDB; 1137416at2759; -.
DR PhylomeDB; Q9M350; -.
DR PRO; PR:Q9M350; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M350; baseline and differential.
DR Genevisible; Q9M350; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000923; C:equatorial microtubule organizing center; IBA:GO_Central.
DR GO; GO:0000930; C:gamma-tubulin complex; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005819; C:spindle; IDA:UniProtKB.
DR GO; GO:0000922; C:spindle pole; IEA:InterPro.
DR GO; GO:0045298; C:tubulin complex; IDA:TAIR.
DR GO; GO:0043015; F:gamma-tubulin binding; IBA:GO_Central.
DR GO; GO:0043622; P:cortical microtubule organization; IMP:TAIR.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0007020; P:microtubule nucleation; IMP:TAIR.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0009624; P:response to nematode; IDA:UniProtKB.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR Gene3D; 1.20.120.1900; -; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR007259; GCP.
DR InterPro; IPR040457; GCP_C.
DR InterPro; IPR042241; GCP_C_sf.
DR InterPro; IPR041470; GCP_N.
DR PANTHER; PTHR19302; PTHR19302; 1.
DR Pfam; PF04130; GCP_C_terminal; 1.
DR Pfam; PF17681; GCP_N_terminal; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Cytoskeleton; Microtubule; Reference proteome.
FT CHAIN 1..745
FT /note="Gamma-tubulin complex component 4"
FT /id="PRO_0000078127"
SQ SEQUENCE 745 AA; 85907 MW; D7DD2EE7E6C2CC4B CRC64;
MLHELLLALL GFTGDLIVDE REQRKTLGLA FNSDSPLSDE CTFKLAPDIS FIEPSERDLI
ERLIKLGFYY RELDRFAKKS RNLSWIRSVT SVHPLERADE LSKQSREKKP SVYRRAIANG
IGEILSVYRS AVLHIEQKLL AETTPILATV TEGLNKFFVL FPPLYEVILE IERDDIRGGQ
LLNVLNKRCH CGVPELRTCL QRLLWNGHQV MYNQLAAWMV YGILQDPHGE FFIKRQDDGD
LDHRSSQEEV SEKLARTSVH ETSLTDWHSG FHISLDMLPD YIPMRLGESI LFAGKAIRVL
RNPSPAFQFQ KDKSFQQTMR GSQRIRGFMH SDFPETETEL DADLTGGELL PQSEADKIEA
MLKDLKESSE FHKRSFECTV DSVRAIAASH LWQLVVVRAD LNGHLKALKD YFLLEKGDFF
QCFLEESRQL MRLPPRQSTG ESDLMVPFQL AATKTIAEED KYFSRVSLRM PSFGVTVRSS
QADMVRSKVS LTGKANLTSD TSVDGWDAIA LEYSVDWPMQ LFFTQEVLSK YLKVFQYLIR
LKRTQMELEK SWASVMHQDH IESAQHRKDG LNGSTSQQRR QGIRPMWRVR EHMAFLIRNL
QFYIQVDVIE SQWKVLQTHI HDSQDFTELV GFHQEYLSAL ISQSFLDIGS VSRILDSIMK
LCLQFCWNIE NQESNPNTSE LENIAEEFNK KSNSLYTILR SSKLAGSQRA PFLRRFLLRL
NFNSFYEATA RGVLNVVRQR PALPL
