ID   GACU_DICDI              Reviewed;        1164 AA.
AC   Q559A0; Q86IF5;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Rho GTPase-activating protein gacU;
DE   AltName: Full=GTPase activating factor for raC protein U;
GN   Name=gacU; ORFNames=DDB_G0272925;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Rho GTPase-activating protein involved in the signal
CC       transduction pathway. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; AAFI02000008; EAL71100.1; -; Genomic_DNA.
DR   RefSeq; XP_644916.1; XM_639824.1.
DR   AlphaFoldDB; Q559A0; -.
DR   SMR; Q559A0; -.
DR   STRING; 44689.DDB0233772; -.
DR   PaxDb; Q559A0; -.
DR   EnsemblProtists; EAL71100; EAL71100; DDB_G0272925.
DR   GeneID; 8618595; -.
DR   KEGG; ddi:DDB_G0272925; -.
DR   dictyBase; DDB_G0272925; gacU.
DR   eggNOG; KOG4270; Eukaryota.
DR   HOGENOM; CLU_274911_0_0_1; -.
DR   InParanoid; Q559A0; -.
DR   OMA; SGEQAFH; -.
DR   PRO; PR:Q559A0; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR   GO; GO:0007264; P:small GTPase mediated signal transduction; IBA:GO_Central.
DR   Gene3D; 1.10.555.10; -; 1.
DR   InterPro; IPR008936; Rho_GTPase_activation_prot.
DR   InterPro; IPR000198; RhoGAP_dom.
DR   Pfam; PF00620; RhoGAP; 1.
DR   SMART; SM00324; RhoGAP; 1.
DR   SUPFAM; SSF48350; SSF48350; 1.
DR   PROSITE; PS50238; RHOGAP; 1.
PE   3: Inferred from homology;
KW   Coiled coil; GTPase activation; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..1164
FT                   /note="Rho GTPase-activating protein gacU"
FT                   /id="PRO_0000380218"
FT   TRANSMEM        279..299
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          56..251
FT                   /note="Rho-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT   REGION          1..46
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          302..329
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          348..371
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          391..468
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          545..581
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          666..768
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          849..1050
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1130..1164
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          627..685
FT                   /evidence="ECO:0000255"
FT   COILED          1057..1130
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..42
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1164 AA;  129609 MW;  3F9DF2484856300B CRC64;
     MKSSKKTLTN FWKDPSTGNL VSPSPSSING GASSSGGGGS TISPTKLAKE GPVFGVPLND
     IFKRNSTNVH NVPNVIWHIS SNIRQSAMDV EGLFRIPGSN TTLQLLKKNY NEGKIGTKDD
     LNDKCDDIHT QASLLKLFIR ELPDPLFTFS LYDSFIKSHG SKDKSIRVGS LKMLLGQLPI
     AHFSLLKFLA ELLREVSTHS VNNRMTSTNL AIVFGPTVMR PQTEDIVKMI EDARHINGIF
     LLIIDEYEFL FNVMYINRNR NININIYLWK SKKNPKISFQ FWNFLFFILV FFFEQPTFAN
     NPSPNTSRNN LNSSVRQSPN KGFVSSNPNR KASKFEFSVV DDVENNSNDI NNNIKLNTNN
     NNNSGKSQQQ LLQQLQMQQL LSISPNSSVI GINQQNANNN SGNNNNNNNN NNSSNNQNKF
     SILLPVNNHP QPTLLAPSSP SSSIYGRNNS NGINNGATSP LPPASPRSSL TLLDMNHLNC
     GVNDYSGGYN NNINNSIGNN ELIRGLIKKT CSILFDKELT SLTDGFEITK DETFIPAFDT
     SNINNHHSSP TSNSVNNNNS NNNNNNNNSS NNNNNTLNSD ISQISPRFSR ANEISSASNS
     STSSPQLRSL LKSHVNNNLN NLNNLPNLLG NLQAQQQQQQ QQQQQQQQQQ QQQQQQQQQQ
     QQQQQQQQLK NQSDENNNVT INNSNNNNNT PTSPVLNIIN NAPITIPSSP PLNTVTSNLS
     PSTPYNESNN NNINNNNNNN NNNNNNNNNN NNNNNNNNIN SNSNNNPLDI AFSHLEKVRK
     EGKRNSDINL MSIDELNEEK SAIKKELRDF DTNFKRQYGQ LPRKNDKEIM RPLYIRYREV
     KSLIDLKSPQ SVTSTLSNSS SLPSTPISLS SSNSLNKSQP SNNNNNNNNN NNNNNNANNT
     NTNNSNNNNN NNNNNNNNNI NNNSNTANMS NSTSTLSSSG QHQLERANSS SSITSAAGIG
     GGSNSKSSTS PPNVVSPYIS PSTSFNSLTE LSNHQNNNNS NNNNNNNNNI DTSAPMIVDS
     HQYNSGNSNS NSNSNSNSNS SSKSSSPNIT DFTKLSLNEL KDRYIKVKGE KRSLQIDLHR
     YQSEFTKKHG RKVQFVEDRV PVQAEYEKYK ELKIEIVALE NILVLINNNH NHNHNHNHNH
     NNNNNNNNNN NNNNNNNHTT LYHL