GACW_DICDI
ID GACW_DICDI Reviewed; 1115 AA.
AC Q54G18;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Rho GTPase-activating protein gacW;
DE AltName: Full=GTPase activating factor for raC protein W;
GN Name=gacW; ORFNames=DDB_G0290439;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Rho GTPase-activating protein involved in the signal
CC transduction pathway. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR EMBL; AAFI02000163; EAL62235.1; -; Genomic_DNA.
DR RefSeq; XP_635754.1; XM_630662.1.
DR AlphaFoldDB; Q54G18; -.
DR SMR; Q54G18; -.
DR STRING; 44689.DDB0233787; -.
DR PaxDb; Q54G18; -.
DR PRIDE; Q54G18; -.
DR EnsemblProtists; EAL62235; EAL62235; DDB_G0290439.
DR GeneID; 8627673; -.
DR KEGG; ddi:DDB_G0290439; -.
DR dictyBase; DDB_G0290439; gacW.
DR eggNOG; KOG4242; Eukaryota.
DR eggNOG; KOG4271; Eukaryota.
DR HOGENOM; CLU_281243_0_0_1; -.
DR InParanoid; Q54G18; -.
DR OMA; PINSFMI; -.
DR PhylomeDB; Q54G18; -.
DR Reactome; R-DDI-983231; Factors involved in megakaryocyte development and platelet production.
DR PRO; PR:Q54G18; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030027; C:lamellipodium; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0016477; P:cell migration; IBA:GO_Central.
DR GO; GO:2000249; P:regulation of actin cytoskeleton reorganization; IBA:GO_Central.
DR GO; GO:0034315; P:regulation of Arp2/3 complex-mediated actin nucleation; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR041245; CARMIL_PH.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF17888; Carm_PH; 1.
DR Pfam; PF13516; LRR_6; 2.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTPase activation; Reference proteome.
FT CHAIN 1..1115
FT /note="Rho GTPase-activating protein gacW"
FT /id="PRO_0000380220"
FT DOMAIN 699..880
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 661..691
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 888..1065
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1092..1115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 888..940
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 956..990
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1006..1045
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1115 AA; 121786 MW; 777AB43674171DD8 CRC64;
MTTPVSLSQV EKKTIESILT NKKEEGKLIT KVTILNEKKK KLEIKTLILS SNRIFLFTDS
KGKLSAEHHY LEITEIASQS ENDVTIKFKN QNVMKMNLDA SEVLKTLYGT LMSTFPGIKI
GKTIIFNITP ASRIPSIFQA TSFKDIQGCG SFNLTYRSVC DHLGVQPLSS ILWDIENLYP
FNQVREFNLA EIYQYSFTDL KAILLSLSYN TYFQSFQSNG KLSNEDLNLL AEVFKENSSL
QQLTITNSQA SKEPIITLLQ NVAENKALHL SHINLNNNSL ENKGILTLGS SIQTFSNGLT
YLNIENTSCS GKGLEAMFGC LTANNVVCGS MTHLNISNNK LESYGTNGLC KFLSKATALQ
QLLMSNTSPV YSFLKTSSSS IQTLDFSGNK STTTKESVID ILSFFKQMSH LQTVNLSRIQ
TTPDDLKLLF SPATSLLKCS SVDLSENDLG DGGIIKLCEI MYPNSNLHHL SIDGNFKTKS
KLRARAIEAL INLIDDNTSI ESLSLASGSG KYQLKGDLMP LLLSLLKNQS LVKLDISGNG
VGDSGALAVS KILWKNQTLK SLKCDGNDFS YTALKMIKCS IRRNEKSLTI LQLPLSDIST
ILKNDSNGSS HEKILKTIQD IQTSIVINYP NNIHPIDTSS GGLTPTKQPS IGSLAGVIKK
PPIGPPSTPT KSSNPGFQTL PKRPTRGPLY PGRATGGAVT LTDKAQINAS TIELPPLVSR
SIDLLMEQGI SSVGIFRTCA SATALKKIKT RFEAGEDIDL KAENVDVDTV AGVLKSYFRE
LPNPIFPENL HEYFFQAMRQ SSNEEIIQSL KDIIDQLSPL ECKMIKKLFH LLHLISLEKD
VNMMSPENIA ICWAPTFFRS FASELLPINS FMIVNYFDIF DPENKPISSD NSGDADTDST
QSTCTTNNNN LVATSLSSPI SPSTTPTKSI DNVLHTTVEV SRSPNHERAH TVGPMTSKRP
ISNNNGVSSN TPPLPNNVTP HHNTMPSRPS ASPIKRPPSM GGSLSNFAGI PLSNHSSSGQ
LNNNNSNNNT TSNSSNSSSG KSSSNPSPIP TPPDSPSMSY IGDGKSTLRS KRFSRVNRVS
YSPVLPRAWT NESRTVSSLF QDDLDSNSDS SGPSL
