ID   GAD1_CAEEL              Reviewed;         620 AA.
AC   O16519;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 162.
DE   RecName: Full=Gastrulation defective protein 1;
GN   Name=gad-1; ORFNames=T05H4.14;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF HIS-324.
RX   PubMed=9659931; DOI=10.1016/s0012-1606(98)80003-1;
RA   Knight J.K., Wood W.B.;
RT   "Gastrulation initiation in Caenorhabditis elegans requires the function of
RT   gad-1, which encodes a protein with WD repeats.";
RL   Dev. Biol. 198:253-265(1998).
CC   -!- FUNCTION: Required for initiation of gastrulation.
CC       {ECO:0000269|PubMed:9659931}.
CC   -!- INTERACTION:
CC       O16519; O45521: nhr-111; NbExp=3; IntAct=EBI-327317, EBI-313236;
CC   -!- DEVELOPMENTAL STAGE: Expressed maternally. Present at high level in
CC       early embryos. In adult hermaphrodites, expression is restricted to the
CC       germline. {ECO:0000269|PubMed:9659931}.
CC   -!- SIMILARITY: Belongs to the WD repeat GAD-1 family. {ECO:0000305}.
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DR   EMBL; FO081501; CCD72040.1; -; Genomic_DNA.
DR   PIR; T31906; T31906.
DR   RefSeq; NP_504635.1; NM_072234.4.
DR   AlphaFoldDB; O16519; -.
DR   SMR; O16519; -.
DR   BioGRID; 44073; 21.
DR   DIP; DIP-27096N; -.
DR   IntAct; O16519; 13.
DR   STRING; 6239.T05H4.14; -.
DR   iPTMnet; O16519; -.
DR   EPD; O16519; -.
DR   PaxDb; O16519; -.
DR   PeptideAtlas; O16519; -.
DR   EnsemblMetazoa; T05H4.14.1; T05H4.14.1; WBGene00001513.
DR   GeneID; 179027; -.
DR   KEGG; cel:CELE_T05H4.14; -.
DR   UCSC; T05H4.14; c. elegans.
DR   CTD; 179027; -.
DR   WormBase; T05H4.14; CE13295; WBGene00001513; gad-1.
DR   eggNOG; KOG0772; Eukaryota.
DR   GeneTree; ENSGT00390000015433; -.
DR   HOGENOM; CLU_014033_1_2_1; -.
DR   InParanoid; O16519; -.
DR   OMA; QGGLRTN; -.
DR   OrthoDB; 729650at2759; -.
DR   PhylomeDB; O16519; -.
DR   PRO; PR:O16519; -.
DR   Proteomes; UP000001940; Chromosome V.
DR   Bgee; WBGene00001513; Expressed in adult organism and 4 other tissues.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0035861; C:site of double-strand break; IBA:GO_Central.
DR   GO; GO:0042074; P:cell migration involved in gastrulation; IMP:WormBase.
DR   GO; GO:0000132; P:establishment of mitotic spindle orientation; IMP:WormBase.
DR   GO; GO:1903775; P:regulation of DNA double-strand break processing; IBA:GO_Central.
DR   GO; GO:0007346; P:regulation of mitotic cell cycle; IMP:WormBase.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF00400; WD40; 3.
DR   SMART; SM00320; WD40; 6.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 2.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..620
FT                   /note="Gastrulation defective protein 1"
FT                   /id="PRO_0000305141"
FT   REPEAT          121..160
FT                   /note="WD 1"
FT   REPEAT          168..207
FT                   /note="WD 2"
FT   REPEAT          222..262
FT                   /note="WD 3"
FT   REPEAT          278..317
FT                   /note="WD 4"
FT   REPEAT          323..362
FT                   /note="WD 5"
FT   REPEAT          415..455
FT                   /note="WD 6"
FT   REGION          28..99
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          593..620
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        28..59
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         324
FT                   /note="H->N: In ct226; temperature-sensitive. In embryos
FT                   from homozygous mutants, hermaphrodites reared at 25
FT                   degrees Celsius, the E cells divide early with abnormal
FT                   spindle orientations and fail to migrate into the embryo,
FT                   and no subsequent gastrulation movements occur."
FT                   /evidence="ECO:0000269|PubMed:9659931"
SQ   SEQUENCE   620 AA;  69046 MW;  2A928EB132A97387 CRC64;
     MDDNTAPKKN SEKKARVFDL QAMFAQTISN APRTEEPTHV PQSVSVSSKS ESGVSSSAVS
     DDEDDFMPDL PPGFQMQQSE AGPSSSRAEG PEDSDDSDFD ETEAISIIKL IPAACEAKIS
     HGTQAISALR VEPPGVRFAS GGLDYYVKLF DFQKMDMSMR YDKELLPAES HVINSLAFSP
     NGETLVVASG EAIIRLLDRA GKQWSETVRG DQYIIDLNIT KGHTATVNCV EFNPLNKNEF
     ISCSDDGSLR LWNLDDHKVI TKCINKHRKV IKTKGANGKR VSPQVCTFSP DGKWIAAGCD
     DGSVQAWKYG SQYVNVNYLV RKAHNGSITS IAFSPDSKRL LSRGFDDTLK MWSLDNPKEP
     LLVKTGLENA FKSTDCGFSP RAEVVFTGTS SPNKDTPGTL QFFDPMTFDL VYKIDYPGIS
     CHRIQWHPRL NQIIAGLSDG TIHVYYDQTM SQRGVMSCVT KPLKRNRASE VVREDMVLSP
     LSLEMFQPRG EEGEEKEVTG WRIKKYLRMQ DNKLRPEFRK PADMPINGKS ANGRVAASGG
     SLHSYLAKQI GTARNAEFLK DTDVRASILK HAKDAEENPL YIDKAYRKTQ PKKIFQETAV
     EPEDQDDEEL QPVFKMPRTK