GADA_DICDI
ID GADA_DICDI Reviewed; 462 AA.
AC Q54VQ5;
DT 09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Glutamate decarboxylase A;
DE EC=4.1.1.15;
GN Name=gadA; ORFNames=DDB_G0280199;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP DEVELOPMENTAL STAGE.
RX PubMed=11553701; DOI=10.1091/mbc.12.9.2590;
RA Iranfar N., Fuller D., Sasik R., Hwa T., Laub M., Loomis W.F.;
RT "Expression patterns of cell-type-specific genes in Dictyostelium.";
RL Mol. Biol. Cell 12:2590-2600(2001).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=16672332; DOI=10.1242/dev.02399;
RA Anjard C., Loomis W.F.;
RT "GABA induces terminal differentiation of Dictyostelium through a GABAB
RT receptor.";
RL Development 133:2253-2261(2006).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=18559084; DOI=10.1186/1471-2164-9-291;
RA Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B.,
RA Skelton J., Ivens A., Bozzaro S.;
RT "Genome-wide transcriptional changes induced by phagocytosis or growth on
RT bacteria in Dictyostelium.";
RL BMC Genomics 9:291-291(2008).
CC -!- FUNCTION: Generates GABA from glutamate. GABA induces the release of
CC acbA from prespore cells and induces the exposure of tagC on the
CC surface of prestalk cells where it can convert acbA to SDF-2. Glutamate
CC acts as a competitive inhibitor. {ECO:0000269|PubMed:16672332}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + L-glutamate = 4-aminobutanoate + CO2;
CC Xref=Rhea:RHEA:17785, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:59888; EC=4.1.1.15;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000250};
CC -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Exclusively expressed in prespore cells late in
CC development. {ECO:0000269|PubMed:16672332}.
CC -!- DEVELOPMENTAL STAGE: Expressed only after 10 hours of development and
CC its mRNA accumulates to peak at 18 hours when cells are initiating
CC fruiting body formation. Highly expressed during phagocytosis of non-
CC pathogenic bacteria. {ECO:0000269|PubMed:11553701,
CC ECO:0000269|PubMed:18559084}.
CC -!- DISRUPTION PHENOTYPE: Mutant cells grow and develop well, forming
CC normally proportioned fruiting bodies, but the number of viable spores
CC is reduced to about half. {ECO:0000269|PubMed:16672332}.
CC -!- SIMILARITY: Belongs to the group II decarboxylase family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000035; EAL67326.1; -; Genomic_DNA.
DR RefSeq; XP_641300.1; XM_636208.1.
DR AlphaFoldDB; Q54VQ5; -.
DR SMR; Q54VQ5; -.
DR STRING; 44689.DDB0231446; -.
DR PaxDb; Q54VQ5; -.
DR EnsemblProtists; EAL67326; EAL67326; DDB_G0280199.
DR GeneID; 8622433; -.
DR KEGG; ddi:DDB_G0280199; -.
DR dictyBase; DDB_G0280199; gadA.
DR eggNOG; KOG1383; Eukaryota.
DR HOGENOM; CLU_019582_2_2_1; -.
DR InParanoid; Q54VQ5; -.
DR OMA; TMAPHSE; -.
DR PhylomeDB; Q54VQ5; -.
DR PRO; PR:Q54VQ5; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0004351; F:glutamate decarboxylase activity; ISS:dictyBase.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0006538; P:glutamate catabolic process; ISS:dictyBase.
DR Gene3D; 3.40.640.10; -; 1.
DR InterPro; IPR010107; Glutamate_decarboxylase.
DR InterPro; IPR002129; PyrdxlP-dep_de-COase.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR PANTHER; PTHR43321; PTHR43321; 1.
DR Pfam; PF00282; Pyridoxal_deC; 1.
DR SUPFAM; SSF53383; SSF53383; 1.
DR TIGRFAMs; TIGR01788; Glu-decarb-GAD; 1.
PE 2: Evidence at transcript level;
KW Lyase; Pyridoxal phosphate; Reference proteome.
FT CHAIN 1..462
FT /note="Glutamate decarboxylase A"
FT /id="PRO_0000391329"
FT BINDING 62
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 83
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 124..125
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT BINDING 210
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT BINDING 273
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT MOD_RES 274
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 462 AA; 52621 MW; 5DFE4AB49DD215CB CRC64;
MSLHHVKTNK YKGFYDSYAS PPATKEISKY SLKEESNKPE VIRDLIIDEL LLDGNAKQNL
ATFCQTDLDK EIHVLMDKCI DKNMIDKDEY PQTAEIESRC VHILADLWNS PESSETIGCS
TTGSSEAAML GGMALKWRWR ENRKKQGKPF DKPNIVTGPV QICWHKFALY FDIELREVPM
EHDRYIMTPE EAIKRCDENT IGVIPTLGVT FTLQYEDVKG ISEALDKFEK ETGLDIPIHV
DAASGGFVAP FLQPEIVWDF RLPRVKSING SGHKFGLSPL GVGWVVWRGK NDIHKDLVFD
VNYLGGNMPT FSLNFSRPGG QIVCQYYNFL RHGRSGYTAV HQACLDHGIF ISKQVKKHGI
FDIVYDGTGA LPGACWKLKK DAKVKFNLFD LSDRMRNRGW QIASYTLPNL PDIVVQRVLI
RHGFSHDMAM LLIEDFNRSI EYFDKHPMVQ SISKDEGQSF HH
