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GADB_DICDI
ID   GADB_DICDI              Reviewed;         463 AA.
AC   Q54IJ3;
DT   09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Glutamate decarboxylase B;
DE            EC=4.1.1.15;
GN   Name=gadB; ORFNames=DDB_G0288715;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=12912885; DOI=10.1128/ec.2.4.664-670.2003;
RA   Iranfar N., Fuller D., Loomis W.F.;
RT   "Genome-wide expression analyses of gene regulation during early
RT   development of Dictyostelium discoideum.";
RL   Eukaryot. Cell 2:664-670(2003).
RN   [3]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=18559084; DOI=10.1186/1471-2164-9-291;
RA   Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B.,
RA   Skelton J., Ivens A., Bozzaro S.;
RT   "Genome-wide transcriptional changes induced by phagocytosis or growth on
RT   bacteria in Dictyostelium.";
RL   BMC Genomics 9:291-291(2008).
CC   -!- FUNCTION: Generates GABA from glutamate. Likely to be responsible for
CC       the metabolism of glutamate via the GABA shunt to succinate during
CC       growth.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + L-glutamate = 4-aminobutanoate + CO2;
CC         Xref=Rhea:RHEA:17785, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:59888; EC=4.1.1.15;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in growing cells, its mRNA continuously
CC       decreases during development. Highly expressed during phagocytosis of
CC       non-pathogenic bacteria. {ECO:0000269|PubMed:12912885,
CC       ECO:0000269|PubMed:18559084}.
CC   -!- SIMILARITY: Belongs to the group II decarboxylase family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000120; EAL63089.1; -; Genomic_DNA.
DR   RefSeq; XP_636593.1; XM_631501.1.
DR   AlphaFoldDB; Q54IJ3; -.
DR   SMR; Q54IJ3; -.
DR   STRING; 44689.DDB0231439; -.
DR   PaxDb; Q54IJ3; -.
DR   EnsemblProtists; EAL63089; EAL63089; DDB_G0288715.
DR   GeneID; 8626767; -.
DR   KEGG; ddi:DDB_G0288715; -.
DR   dictyBase; DDB_G0288715; gadB.
DR   eggNOG; KOG1383; Eukaryota.
DR   HOGENOM; CLU_019582_2_1_1; -.
DR   InParanoid; Q54IJ3; -.
DR   OMA; IIWRDES; -.
DR   PhylomeDB; Q54IJ3; -.
DR   PRO; PR:Q54IJ3; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR   GO; GO:0004351; F:glutamate decarboxylase activity; ISS:dictyBase.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR   GO; GO:0006538; P:glutamate catabolic process; ISS:dictyBase.
DR   Gene3D; 3.40.640.10; -; 1.
DR   InterPro; IPR010107; Glutamate_decarboxylase.
DR   InterPro; IPR002129; PyrdxlP-dep_de-COase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   PANTHER; PTHR43321; PTHR43321; 1.
DR   Pfam; PF00282; Pyridoxal_deC; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   TIGRFAMs; TIGR01788; Glu-decarb-GAD; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Pyridoxal phosphate; Reference proteome.
FT   CHAIN           1..463
FT                   /note="Glutamate decarboxylase B"
FT                   /id="PRO_0000391330"
FT   BINDING         60
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         81
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         122..123
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000250"
FT   BINDING         208
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000250"
FT   BINDING         271
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         272
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   463 AA;  52506 MW;  8803CBD7A216B4E3 CRC64;
     MPLHIVDKHK SLYDNYVQSA TTEEIAKFKL ADDPNEPETI KKLIMDELLL DGNSKQNLAT
     FCQTDLDKNI HSIMDKCIDK NMIDKDEYPQ SAEIENRCLH ILADLWNAPD SSDTIGCSTT
     GSSEAAMLGG LALKWNWREN RKKLGLPYDK PNIVTGPVQI CWHKFALYFD IEIREIPMEN
     GRYVMNSEEV LKRVDENTIG VIPTLGVTFT LQYEDVFSIS NALDKFEKES GINIPIHVDA
     ASGGFVAPFI QQEIIWDFRL PRVKSINASG HKFGLSPLGV GWVVWREKKD LHKDLVFNVN
     YLGGNMSTFS LNFSRPGGQI IAQYYNFLRH GRNGYTLIQD ACAQQGIFIG KELKKMGIFD
     LIYDGTGALP GVCWTISKNL PSEPLFNLYD LSEKLRSRGW QVASYSLPSS MNDVVVSRVV
     IRHGFSRDLS SLFVKDVQNA IEYFQKHPIL RSLEKDEGEN FHH
 
 
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