ID   GADE_ECO57              Reviewed;         175 AA.
AC   P63206; P29688;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Transcriptional regulator GadE;
GN   Name=gadE; OrderedLocusNames=Z4925, ECs4392;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [3]
RP   FUNCTION.
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=12704134; DOI=10.1128/iai.71.5.2598-2606.2003;
RA   Tatsuno I., Nagano K., Taguchi K., Rong L., Mori H., Sasakawa C.;
RT   "Increased adherence to Caco-2 cells caused by disruption of the yhiE and
RT   yhiF genes in enterohemorrhagic Escherichia coli O157:H7.";
RL   Infect. Immun. 71:2598-2606(2003).
CC   -!- FUNCTION: Regulates the expression of several genes involved in acid
CC       resistance. Required for the expression of gadA and gadBC, among
CC       others, regardless of media or growth conditions. Binds directly to the
CC       20 bp GAD box found in the control regions of both loci (By
CC       similarity). Could be involved in the regulation of the genes coding
CC       for the type III secretion system in enterohaemorragic strains.
CC       {ECO:0000250, ECO:0000269|PubMed:12704134}.
CC   -!- INDUCTION: By acidic conditions. Could be induced by EvgA via the
CC       induction of YdeO (By similarity). {ECO:0000250}.
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DR   EMBL; AE005174; AAG58653.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37815.1; -; Genomic_DNA.
DR   PIR; A86024; A86024.
DR   PIR; H91177; H91177.
DR   RefSeq; NP_312419.1; NC_002695.1.
DR   RefSeq; WP_000576690.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P63206; -.
DR   SMR; P63206; -.
DR   STRING; 155864.EDL933_4762; -.
DR   EnsemblBacteria; AAG58653; AAG58653; Z4925.
DR   EnsemblBacteria; BAB37815; BAB37815; ECs_4392.
DR   GeneID; 66672604; -.
DR   GeneID; 915751; -.
DR   KEGG; ece:Z4925; -.
DR   KEGG; ecs:ECs_4392; -.
DR   PATRIC; fig|386585.9.peg.4589; -.
DR   eggNOG; COG2771; Bacteria.
DR   HOGENOM; CLU_1522892_0_0_6; -.
DR   OMA; INNISDM; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00196; GerE; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..175
FT                   /note="Transcriptional regulator GadE"
FT                   /id="PRO_0000184157"
FT   DOMAIN          109..174
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        133..152
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
SQ   SEQUENCE   175 AA;  20599 MW;  5CCC69A0DA53B293 CRC64;
     MIFLMTKDSF LLQGFWQLKD NHEMIKINSL SEIKKVGNKP FKVIIDTYHN HILDEEAIKF
     LEKLDAERII VLAPYHISKL KAKAPIYFVS RKESIKNLLE ITYGKHLPHK NSQLCFSHNQ
     FKIMQLILKN KNESNITSTL NISQQTLKIQ KFNIMYKLKL RRMSDIVTLG ITSYF