GADE_ECOLI
ID GADE_ECOLI Reviewed; 175 AA.
AC P63204; P29688; Q2M7H4;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Transcriptional regulator GadE;
GN Name=gadE; Synonyms=yhiE, yhiT; OrderedLocusNames=b3512, JW3480;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-111.
RC STRAIN=K12;
RX PubMed=8244952; DOI=10.1128/jb.175.23.7747-7748.1993;
RA Yoshida T., Ueguchi C., Mizuno T.;
RT "Physical map location of a set of Escherichia coli genes (hde) whose
RT expression is affected by the nucleoid protein H-NS.";
RL J. Bacteriol. 175:7747-7748(1993).
RN [5]
RP INDUCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=12399493; DOI=10.1128/jb.184.22.6225-6234.2002;
RA Masuda N., Church G.M.;
RT "Escherichia coli gene expression responsive to levels of the response
RT regulator EvgA.";
RL J. Bacteriol. 184:6225-6234(2002).
RN [6]
RP INDUCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=12694615; DOI=10.1046/j.1365-2958.2003.03477.x;
RA Masuda N., Church G.M.;
RT "Regulatory network of acid resistance genes in Escherichia coli.";
RL Mol. Microbiol. 48:699-712(2003).
RN [7]
RP FUNCTION.
RC STRAIN=K12;
RX PubMed=12940989; DOI=10.1046/j.1365-2958.2003.03633.x;
RA Ma Z., Gong S., Richard H., Tucker D.L., Conway T., Foster J.W.;
RT "GadE (YhiE) activates glutamate decarboxylase-dependent acid resistance in
RT Escherichia coli K-12.";
RL Mol. Microbiol. 49:1309-1320(2003).
RN [8]
RP FUNCTION.
RC STRAIN=FB8;
RX PubMed=14702398; DOI=10.1099/mic.0.26659-0;
RA Hommais F., Krin E., Coppee J.-Y., Lacroix C., Yeramian E., Danchin A.,
RA Bertin P.;
RT "GadE (YhiE): a novel activator involved in the response to acid
RT environment in Escherichia coli.";
RL Microbiology 150:61-72(2004).
RN [9]
RP PROTEIN SEQUENCE OF 46-59, AND INDUCTION BY HEAT SHOCK.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=29039649; DOI=10.1021/acs.biochem.7b00864;
RA Yuan P., D'Lima N.G., Slavoff S.A.;
RT "Comparative membrane proteomics reveals a nonannotated E. coli heat shock
RT protein.";
RL Biochemistry 57:56-60(2018).
CC -!- FUNCTION: Regulates the expression of several genes involved in acid
CC resistance. Required for the expression of gadA and gadBC, among
CC others, regardless of media or growth conditions. Binds directly to the
CC 20 bp GAD box found in the control regions of both loci.
CC {ECO:0000269|PubMed:12940989, ECO:0000269|PubMed:14702398}.
CC -!- INDUCTION: By acidic conditions (PubMed:12694615). Could be induced by
CC EvgA via the induction of YdeO (PubMed:12399493). By heat shock (shift
CC from 30 to 45 degrees Celsius) (at protein level) (PubMed:29039649).
CC {ECO:0000269|PubMed:12399493, ECO:0000269|PubMed:12694615,
CC ECO:0000269|PubMed:29039649}.
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DR EMBL; U00039; AAB18488.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76537.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77782.1; -; Genomic_DNA.
DR EMBL; D11389; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S47732; S47732.
DR RefSeq; NP_417969.1; NC_000913.3.
DR RefSeq; WP_000576690.1; NZ_STEB01000046.1.
DR AlphaFoldDB; P63204; -.
DR SMR; P63204; -.
DR BioGRID; 4262519; 8.
DR ComplexPortal; CPX-5781; gadE-rcsB DNA-binding transcription factor complex.
DR DIP; DIP-48091N; -.
DR IntAct; P63204; 2.
DR STRING; 511145.b3512; -.
DR jPOST; P63204; -.
DR PaxDb; P63204; -.
DR PRIDE; P63204; -.
DR EnsemblBacteria; AAC76537; AAC76537; b3512.
DR EnsemblBacteria; BAE77782; BAE77782; BAE77782.
DR GeneID; 66672604; -.
DR GeneID; 948023; -.
DR KEGG; ecj:JW3480; -.
DR KEGG; eco:b3512; -.
DR PATRIC; fig|511145.12.peg.3619; -.
DR EchoBASE; EB1506; -.
DR eggNOG; COG2771; Bacteria.
DR HOGENOM; CLU_1522892_0_0_6; -.
DR OMA; INNISDM; -.
DR BioCyc; EcoCyc:EG11544-MON; -.
DR PRO; PR:P63204; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005667; C:transcription regulator complex; IPI:ComplexPortal.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:1990451; P:cellular stress response to acidic pH; IDA:ComplexPortal.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:ComplexPortal.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00196; GerE; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
PE 1: Evidence at protein level;
KW Activator; Direct protein sequencing; DNA-binding; Reference proteome;
KW Stress response; Transcription; Transcription regulation.
FT CHAIN 1..175
FT /note="Transcriptional regulator GadE"
FT /id="PRO_0000184155"
FT DOMAIN 109..174
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 133..152
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
SQ SEQUENCE 175 AA; 20599 MW; 5CCC69A0DA53B293 CRC64;
MIFLMTKDSF LLQGFWQLKD NHEMIKINSL SEIKKVGNKP FKVIIDTYHN HILDEEAIKF
LEKLDAERII VLAPYHISKL KAKAPIYFVS RKESIKNLLE ITYGKHLPHK NSQLCFSHNQ
FKIMQLILKN KNESNITSTL NISQQTLKIQ KFNIMYKLKL RRMSDIVTLG ITSYF
