ID   GADE_SHIFL              Reviewed;         175 AA.
AC   P63207; P29688;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2004, sequence version 1.
DT   25-MAY-2022, entry version 111.
DE   RecName: Full=Transcriptional regulator GadE;
GN   Name=gadE; OrderedLocusNames=SF3546, S4221;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Regulates the expression of several genes involved in acid
CC       resistance. Required for the expression of gadA and gadBC, among
CC       others, regardless of media or growth conditions. Binds directly to the
CC       20 bp GAD box found in the control regions of both loci (By
CC       similarity). {ECO:0000250}.
CC   -!- INDUCTION: By acidic conditions. Could be induced by EvgA via the
CC       induction of YdeO (By similarity). {ECO:0000250}.
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DR   EMBL; AE005674; AAN45001.2; -; Genomic_DNA.
DR   EMBL; AE014073; AAP19185.1; -; Genomic_DNA.
DR   RefSeq; NP_709294.2; NC_004337.2.
DR   RefSeq; WP_000576690.1; NZ_WPGV01000129.1.
DR   AlphaFoldDB; P63207; -.
DR   SMR; P63207; -.
DR   STRING; 198214.SF3546; -.
DR   EnsemblBacteria; AAN45001; AAN45001; SF3546.
DR   EnsemblBacteria; AAP19185; AAP19185; S4221.
DR   GeneID; 1026396; -.
DR   GeneID; 66672604; -.
DR   KEGG; sfl:SF3546; -.
DR   KEGG; sfx:S4221; -.
DR   PATRIC; fig|198214.7.peg.4176; -.
DR   HOGENOM; CLU_1522892_0_0_6; -.
DR   OMA; INNISDM; -.
DR   OrthoDB; 1579577at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00196; GerE; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..175
FT                   /note="Transcriptional regulator GadE"
FT                   /id="PRO_0000184158"
FT   DOMAIN          109..174
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        133..152
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
SQ   SEQUENCE   175 AA;  20599 MW;  5CCC69A0DA53B293 CRC64;
     MIFLMTKDSF LLQGFWQLKD NHEMIKINSL SEIKKVGNKP FKVIIDTYHN HILDEEAIKF
     LEKLDAERII VLAPYHISKL KAKAPIYFVS RKESIKNLLE ITYGKHLPHK NSQLCFSHNQ
     FKIMQLILKN KNESNITSTL NISQQTLKIQ KFNIMYKLKL RRMSDIVTLG ITSYF