ID   GADW_ECO57              Reviewed;         242 AA.
AC   P63202; P37638;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=HTH-type transcriptional regulator GadW;
GN   Name=gadW; OrderedLocusNames=Z4928, ECs4395;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Depending on the conditions (growth phase and medium), acts
CC       as a positive or negative regulator of gadA and gadBC. Repression
CC       occurs directly or via the repression of the expression of gadX.
CC       Activation occurs directly by the binding of GadW to the gadA and gadBC
CC       promoters (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- INDUCTION: Expression can be repressed by GadX, depending on the
CC       conditions. {ECO:0000250}.
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DR   EMBL; AE005174; AAG58656.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37818.1; -; Genomic_DNA.
DR   PIR; C91178; C91178.
DR   PIR; D86024; D86024.
DR   RefSeq; NP_312422.1; NC_002695.1.
DR   RefSeq; WP_000149999.1; NZ_SEKU01000003.1.
DR   AlphaFoldDB; P63202; -.
DR   STRING; 155864.EDL933_4766; -.
DR   EnsemblBacteria; AAG58656; AAG58656; Z4928.
DR   EnsemblBacteria; BAB37818; BAB37818; ECs_4395.
DR   GeneID; 58391716; -.
DR   GeneID; 915748; -.
DR   KEGG; ece:Z4928; -.
DR   KEGG; ecs:ECs_4395; -.
DR   PATRIC; fig|386585.9.peg.4593; -.
DR   eggNOG; COG2207; Bacteria.
DR   HOGENOM; CLU_000445_81_4_6; -.
DR   OMA; EIDECIV; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..242
FT                   /note="HTH-type transcriptional regulator GadW"
FT                   /id="PRO_0000194516"
FT   DOMAIN          139..236
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        156..177
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        203..226
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ   SEQUENCE   242 AA;  28028 MW;  40C11B74DA2F8DA5 CRC64;
     MTHVCSVILI RRSFDIYHEQ QKISLHNESI LLLEKNLADD FAFCSPDTRR LDIDELTVCH
     YLQNIRQLPR NLGLHSKDRL LINQSPPMPL VTAIFDSFNE SGVNSPILSN MLYLSCLSMF
     SHKKELIPLL FNSISTVSGK VERLISFDIA KRWYLRDIAE RMYTSESLIK KKLQDENTCF
     SKILLASRMS MARRLLELRQ IPLHTIAEKC GYSSTSYFIN TFRQYYGVTP HQFAQHSPGT
     FS