ID   GADW_ECOL6              Reviewed;         242 AA.
AC   Q8FCI7;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=HTH-type transcriptional regulator GadW;
GN   Name=gadW; OrderedLocusNames=c4326;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Depending on the conditions (growth phase and medium), acts
CC       as a positive or negative regulator of gadA and gadBC. Repression
CC       occurs directly or via the repression of the expression of gadX.
CC       Activation occurs directly by the binding of GadW to the gadA and gadBC
CC       promoters (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- INDUCTION: Expression can be repressed by GadX, depending on the
CC       conditions. {ECO:0000250}.
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DR   EMBL; AE014075; AAN82762.1; -; Genomic_DNA.
DR   RefSeq; WP_000951553.1; NC_004431.1.
DR   AlphaFoldDB; Q8FCI7; -.
DR   SMR; Q8FCI7; -.
DR   STRING; 199310.c4326; -.
DR   DNASU; 1037214; -.
DR   EnsemblBacteria; AAN82762; AAN82762; c4326.
DR   KEGG; ecc:c4326; -.
DR   eggNOG; COG2207; Bacteria.
DR   HOGENOM; CLU_000445_81_4_6; -.
DR   OMA; EIDECIV; -.
DR   BioCyc; ECOL199310:C4326-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..242
FT                   /note="HTH-type transcriptional regulator GadW"
FT                   /id="PRO_0000194515"
FT   DOMAIN          139..236
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        156..177
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        203..226
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ   SEQUENCE   242 AA;  28113 MW;  9EDD838B01B97E8C CRC64;
     MAHVCSVILV RRSFDIHHEQ QKISLHNESI LLLDKNLADD FAFCSLDTRR LDIEELTVCH
     YLQNIRQLPR NLGLHSKDRL LINQSPPIQL VTAIFDSFND PRVNSPILSK MLYLSCLSMF
     SHKKELIPLL FNSISTVSGK VERLISFDIA KRWYLRDIAE RMYTSESLIK KKLQDENTCF
     SKILLASRMS MARRLLELRQ IPLHTIAEKC GYSSTSYFIN TFRQYYGVTP HQFSQHSPGT
     FS