GADW_ECOLI
ID GADW_ECOLI Reviewed; 242 AA.
AC P63201; P37638; Q2M7H7;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=HTH-type transcriptional regulator GadW;
GN Name=gadW; Synonyms=yhiW; OrderedLocusNames=b3515, JW3483;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=K12;
RX PubMed=12446650; DOI=10.1128/jb.184.24.7001-7012.2002;
RA Ma Z., Richard H., Tucker D.L., Conway T., Foster J.W.;
RT "Collaborative regulation of Escherichia coli glutamate-dependent acid
RT resistance by two AraC-like regulators, GadX and GadW (YhiW).";
RL J. Bacteriol. 184:7001-7012(2002).
RN [5]
RP FUNCTION.
RC STRAIN=K12;
RX PubMed=12730179; DOI=10.1128/jb.185.10.3190-3201.2003;
RA Tucker D.L., Tucker N., Ma Z., Foster J.W., Miranda R.L., Cohen P.S.,
RA Conway T.;
RT "Genes of the GadX-GadW regulon in Escherichia coli.";
RL J. Bacteriol. 185:3190-3201(2003).
RN [6]
RP FUNCTION, AND INDUCTION.
RX PubMed=14617649; DOI=10.1128/jb.185.23.6852-6859.2003;
RA Ma Z., Richard H., Foster J.W.;
RT "pH-dependent modulation of cyclic AMP levels and GadW-dependent repression
RT of RpoS affect synthesis of the GadX regulator and Escherichia coli acid
RT resistance.";
RL J. Bacteriol. 185:6852-6859(2003).
CC -!- FUNCTION: Depending on the conditions (growth phase and medium), acts
CC as a positive or negative regulator of gadA and gadBC. Repression
CC occurs directly or via the repression of the expression of gadX.
CC Activation occurs directly by the binding of GadW to the gadA and gadBC
CC promoters. {ECO:0000269|PubMed:12446650, ECO:0000269|PubMed:12730179,
CC ECO:0000269|PubMed:14617649}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12446650}.
CC -!- INDUCTION: Expression can be repressed by GadX, depending on the
CC conditions. {ECO:0000269|PubMed:14617649}.
CC -!- MISCELLANEOUS: GadX and GadW are part of a complex system required for
CC acid resistance via the regulation of gadA and gadBC. Many of the
CC described circuits use mutants missing one of the network components.
CC In the wild-type situation, however, many of the scenarios are not
CC easily observed.
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DR EMBL; U00039; AAB18491.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76540.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77779.1; -; Genomic_DNA.
DR PIR; S47735; S47735.
DR RefSeq; NP_417972.1; NC_000913.3.
DR RefSeq; WP_000149999.1; NZ_SSZK01000042.1.
DR AlphaFoldDB; P63201; -.
DR BioGRID; 4262522; 8.
DR IntAct; P63201; 6.
DR STRING; 511145.b3515; -.
DR PaxDb; P63201; -.
DR PRIDE; P63201; -.
DR EnsemblBacteria; AAC76540; AAC76540; b3515.
DR EnsemblBacteria; BAE77779; BAE77779; BAE77779.
DR GeneID; 58391716; -.
DR GeneID; 948029; -.
DR KEGG; ecj:JW3483; -.
DR KEGG; eco:b3515; -.
DR PATRIC; fig|1411691.4.peg.3203; -.
DR EchoBASE; EB2153; -.
DR eggNOG; COG2207; Bacteria.
DR HOGENOM; CLU_000445_81_4_6; -.
DR InParanoid; P63201; -.
DR OMA; EIDECIV; -.
DR PhylomeDB; P63201; -.
DR BioCyc; EcoCyc:EG12242-MON; -.
DR PRO; PR:P63201; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:EcoCyc.
DR GO; GO:0006351; P:transcription, DNA-templated; IMP:EcoCyc.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..242
FT /note="HTH-type transcriptional regulator GadW"
FT /id="PRO_0000194514"
FT DOMAIN 139..236
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 156..177
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 203..226
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ SEQUENCE 242 AA; 28028 MW; 40C11B74DA2F8DA5 CRC64;
MTHVCSVILI RRSFDIYHEQ QKISLHNESI LLLEKNLADD FAFCSPDTRR LDIDELTVCH
YLQNIRQLPR NLGLHSKDRL LINQSPPMPL VTAIFDSFNE SGVNSPILSN MLYLSCLSMF
SHKKELIPLL FNSISTVSGK VERLISFDIA KRWYLRDIAE RMYTSESLIK KKLQDENTCF
SKILLASRMS MARRLLELRQ IPLHTIAEKC GYSSTSYFIN TFRQYYGVTP HQFAQHSPGT
FS
